中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 121-124.doi: 10.3969/j.issn.1673-8225.2012.01.026

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

鱼卵提取物微量刺激法诱导鼠脾细胞表达干细胞标志抗原*☆

阮光萍，姚  翔，庞荣清，王金祥，马丽花，王  强，邓永丽，潘兴华   

  1. 解放军昆明总医院干细胞与组织器官工程研究中心，云南省昆明市   650032
  • 收稿日期:2011-05-21 修回日期:2011-07-04 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 潘兴华，主任医师，解放军昆明总医院干细胞与组织器官工程研究中心，云南省昆明市 650032 xinghuapan@yahoo.com.cn
  • 作者简介:阮光萍☆，女，1974年生，云南省昆明市人，汉族，2007年南方医科大学毕业，博士，主治医师，主要从事干细胞的基础与应用研究。 ruangp@126.com
  • 基金资助:

    云南省社会发展科技计划项目：天然活性物质诱导成体细胞分化为多能干细胞的研究

A micro-stimulation method based on fish oocytes extracts induces mouse spleen cells to express stem cell mark antigen

Ruan Guang-ping, Yao Xiang, Pang Rong-qing, Wang Jin-xiang, Ma Li-hua, Wang Qiang, Deng Yong-li, Pan Xing-hua   

  1. Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming  650032, Yunnan Province, China
  • Received:2011-05-21 Revised:2011-07-04 Online:2012-01-01 Published:2012-01-01
  • Contact: Pan Xing-hua, Chief physician, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China xinghuapan@yahoo.com.cn
  • About author:Ruan Guang-ping☆, Doctor, Attending physician, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China ruangp@126.com
  • Supported by:

    the Social Developmental Program of Yunnan Province*

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摘要：

背景：小鼠和人的体细胞能被一些特殊的因子诱导，逆转为类似胚胎细胞的状态，即诱导性多能干细胞。

目的：进一步提高鱼卵提取物诱导体细胞逆向分化为多能干细胞的效率。
方法：以鱼卵提取物诱导BALB/C小鼠脾细胞分化为多能干细胞，分别以普通诱导法(鱼卵提取物质量浓度分别为0，0.1，0.2，0.4，0.8，1.2 g/L)与微量刺激法诱导(鱼卵提取物质量浓度分别为0，0.05，0.1，0.2，0.4 g/L)。
结果与结论：与普通诱导法比较，微量刺激法诱导的脾细胞表达更多的干细胞标志抗原OCT-3/4，Nanog，SSEA-1。证明微量刺激法可进一步提高体细胞逆向分化为多能干细胞的效率。
关键词：多能干细胞；天然活性物质；脾细胞；干细胞标志抗原；微量刺激法
doi:10.3969/j.issn.1673-8225.2012.01.026

关键词:

Abstract:

BACKGROUND: The mouse and human somatic cells can be induced by some special factors to a state of embryonic cells called as induced pluripotent stem cells.
OBJECTIVE: To further enhance the efficiency of fish oocytes extracts on inducing somatic cells to differentiate into pluripotent stem cells.
METHODS: We compared a new method (micro-stimulation, 0, 0.05, 0.1, 0.2, 0.4 g/L fish oocytes extracts) to improve the induction efficiency, and the traditional method (0, 0.1, 0.2, 0.4, 0.8, 1.2 g/L fish oocytes extracts) in cultured cells by adding fish oocytes extracts.
RESULTS AND CONCLUSION: Compared with the traditional method, the new micro-stimulation method could induce spleen cells to express more stem cell marker antigens OCT-3/4, Nanog, SSEA-1. It is proved that the micro-stimulation method is a more efficient way to generate pluripotent stem cells from somatic cells.

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