中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 10035-10038.doi: 10.3969/j.issn.1673-8225.2011.53.038

• 器官移植学术探讨 academic discussion of organ transplantation • 上一篇    下一篇

不同保存方法对同种异体软骨移植修复软骨缺损的免疫原性影响

王丽霞1，李  敏1，张瑞存1，王爱博2   

  1. 1石家庄学院体育系，河北省石家庄市 050035
    2衡水学院体育学院，河北省衡水市053000
  • 收稿日期:2011-10-21 修回日期:2011-11-30 出版日期:2011-12-31 发布日期:2011-12-31
  • 作者简介:王丽霞★，女，1979年生，河北省曲周县人，汉族，2006年河北师范大学体育学院毕业，硕士，讲师，主要从事运动医学、体育教育训练学研究。 Wanglx79@126.com

Effects of different preservation methods on the immunogenicity of cartilage allografts in the repair of cartilage defects

Wang Li-xia1, Li Min1, Zhang Rui-cun1, Wang Ai-bo2   

  1. 1Department of Physical Education, Shijiazhuang University, Shijiazhuang  050035, Hebei Province, China
    2School of Physical Education, Hengshui University, Hengshui  053000, Hebei Province, China
  • Received:2011-10-21 Revised:2011-11-30 Online:2011-12-31 Published:2011-12-31
  • About author:Wang Li-xia★, Master, Lecturer, Department of Physical Education, Shijiazhuang University, Shijiazhuang 050035, Hebei Province, China Wanglx79@126.com

PDF

453

被引次数

6

摘要：

背景：同种异体骨软骨移植在修复软骨缺损中仍存在免疫排斥等问题。
目的：评价程序深低温冷冻保存法、低温保护剂程序降温保存法、组织培养法、化学处理法对软骨移植物免疫原性的影响。
方法：以“软骨、保存、移植”为中文关键词，采用电子检索的方式，在万方数据库、PubMed数据库中检索1998-01/2011-11关于不同保存方法对软骨移植物免疫原性影响的文章。排除重复研究、普通综述或Meta分析类文章，筛选纳入36篇文献进行评价。
结果与结论：软骨细胞膜上带有组织相容性抗原，软骨细胞能够表达主要相容性复合物且有特异性分化抗原。基质完整时，软骨细胞不会与受体淋巴细胞及浆细胞接触，表现为低免疫性，但软骨下骨组织无基质包绕，所以带有软骨下骨的移植物虽可增加固定的牢固性，但也会增加免疫原性。从不同保存方法对同种异体软骨移植修复软骨缺损的免疫原性影响来看，程序深低温冷冻保存法、低温保护剂程序降温保存法、组织培养法3种方法均有较好的应用效果。

关键词: 软骨, 保存, 移植, 免疫原性, 低温冷冻

Abstract:

BACKGROUND: Immune rejections still exist in the allograft cartilage transplantation for repair of cartilage defects.
OBJECTIVE: To evaluate the effects of programmed cryopreservation method, cryoprotective agent process for cooling preservation, tissue culture method, and chemical treatment on the immunogenicity of cartilage allografts in the repair of cartilage defects.
METHODS: An electronic search of Wanfang and PubMed was performed for articles about effects of different preservation methods on the immunogenicity of cartilage allografts in the repair of cartilage defects published 1998-01/2011-11. The keywords were “cartilage, preservation, transplantation”. Repetitive studies, reviews and Meta analysis were excluded, and finally 36 articles were included.
RESULTS AND CONCLUSION: Chondrocyte membrane has histocompatibility antigens, and chondrocytes can be expressed in the major compatibility complex with specific differentiation antigens. When the matrix is tact, the cartilage cells cannot touch receptor lymphocytes and plasma cells, showing a low immunity, but the subchondral bone was not covered with the matrix. As a result, the cartilage allografts with the subchondral bone can immunogenicity, though the fixation can be strengthened. The programmed cryopreservation method, cryoprotective agent process for cooling preservation, and tissue culture method have achieved good effects on the immunogenicity of cartilage allografts in the repair of cartilage defects.

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