中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9445-9448.doi: 10.3969/j.issn.1673-8225.2011.50.033

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

邻苯二甲酸二丁酯诱导孕鼠尿道下裂模型后仔鼠睾丸细胞过氧化物酶6的表达

沈百欣1，卫中庆1，居小兵2，左  维1，何  佳1，史华超1，张  炜2，王心如3   

  1. 1南京医科大学第二附属医院泌尿外科，江苏省南京市，210011
    2南京医科大学第一附属医院泌尿外科，江苏省南京市 210029
    3南京医科大学公共卫生学院，江苏省南京市 210029
  • 收稿日期:2011-09-22 修回日期:2011-10-21 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 卫中庆，教授，硕士生导师，南京医科大学第二附属医院泌尿外科，江苏省南京市 210011 weizq1@163.com
  • 作者简介:沈百欣★，男，江苏省如皋市人，汉族，2008年南京医科大学毕业，硕士，主要从事环境内化学污染物对男性生殖系统毒理机制及排尿障碍疾病研究。 afantidoctor@163.com
  • 基金资助:

    国家自然科学基金面上项目(30872596)；南京医科大学科技基金面上项目(08NMUM023)；江苏省高等学校大学生实践创新训练计划项目(KY112J2010002)。

Expression of peroxidase 6 in fetal rat testicular cells in pregnant rat hypospadias model induced by di-n-butyl phthalate

Shen Bai-xin1, Wei Zhong-qing1, Ju Xiao-bing2, Zuo Wei1, He Jia1, Shi Hua-chao1, Zhang Wei2, Wang Xin-ru3   

  1. 1Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing  210011, Jiangsu Province, China
    2Department of Urinary Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
    3Nanjing Medical University School of Public Health, Nanjing 210029, Jiangsu Province, China
  • Received:2011-09-22 Revised:2011-10-21 Online:2011-12-10 Published:2011-12-10
  • Contact: Wei Zhong-qing, Professor, Master’s supervisor, Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China weizq1@163.com
  • About author:Shen Bai-xin★, Master, Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China afantidoctor@163.com
  • Supported by:

    the General Project of National Natural Science Foundation of China, No. 30872596*; the General Project of Science and Technology Foundation for Nanjing Medical University, No. 08NMUM023*; the College Students' Practice Innovation Training Project for Higher Education of Jiangsu Province, No. KY112J2010002*

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摘要：

背景：前期实验发现在邻苯二甲酸二丁酯孕期暴露诱导的仔鼠睾丸发育中蛋白过氧化物酶6表达显著增高。
目的：进一步验证邻苯二甲酸二丁酯胚胎期暴露对胎鼠睾丸蛋白过氧化物酶6表达的影响。
方法：妊娠14~18 d按800 mg/(kg•d) 邻苯二甲酸二丁酯染毒孕鼠，妊娠21 d取出胚胎大鼠睾丸，提取蛋白，通过Western blotting及免疫组织化学方法检测睾丸细胞过氧化物酶6的表达，以正常雄性仔鼠睾丸为对照。
结果与结论：Western blotting检测显示，实验组过氧化物酶6的相对表达量为0.205±0.020，对照组为0.110±0.020，实验组明显高于对照组(P < 0.05)。免疫组织化学方法发现过氧化物酶6主要定位于胎鼠睾丸Leydig细胞中。说明过氧化物酶6可能通过发挥抗氧化作用及刺激Leydig细胞增殖功能抵抗邻苯二甲酸二丁酯的毒性作用，其功能有待进一步研究。

关键词: 邻苯二甲酸二丁酯, 大鼠, 睾丸, 过氧化物酶6, 组织构建实验造模

Abstract:

BACKGROUND: Previous study shows that expression of peroxidase 6 in fetal rat testis increase significantly induced by in utero exposure to di-n-butyl phthalate.
OBJECTIVE: To further investigate the effect of prenatal di-n-butyl phthalate exposure on peroxidase 6 expression.
METHODS: Rats of 14 to 18 days of pregnancy were exposed to di-n-butyl phthalate with the dose of 800mg / (kg•d). Testes were harvested from the fetal rats from gestational day 21 and peroxidase 6 was extracted. The expression of peroxidase 6 was detected and analyzed by western blot and immunohistochemical methods.
RESULTS AND CONCLUSION: According to western blot, the expression of peroxidase 6 in experiment group (0.205±0.020) was significantly higher than that in the control group (0.110 ± 0.020, P < 0.05). According to immunohistochemistry, peroxidase 6 was mainly localized in Leydig cells of fetal rat testes. These findings indicate that peroxidase 6 may antagonize di-n-butyl phthalate toxicity by antioxidation and stimulating the proliferation of Leydig cells. The function of peroxidase 6 needs further research.

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