中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9344-9347.doi: 10.3969/j.issn.1673-8225.2011.50.009

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

人胸腺组织原代培养细胞与人类免疫缺陷病毒间的相互作用

高楠竣1，陈英杰2，李雪涛3，孔维云3，周轶平4   

  1. 1云南省玉溪市第三人民医院儿科，云南省玉溪市  653100
    2昆明医学院人体解剖学教研室，云南省昆明市 650031
    3云南省第二人民医院骨科，云南省昆明市650051
    4昆明医学院药学院暨重点实验室，云南省昆明市  650031
  • 收稿日期:2011-09-06 修回日期:2011-10-09 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 周轶平，博士，副教授，昆明医学院药学院暨重点实验室，云南省昆明市 650031 1401545363@qq.com 并列通讯作者：孔维云，在读博士，主治医师，云南省第二人民医院骨科，云南省昆明市650051 Kongweiyunkm128@126.com
  • 作者简介:高楠竣，1966年生，女，汉族，云南省玉溪市人，1989年昆明医学院毕业，副主任医师，从事从事医学生物学研究。 761470408@qq.com 并列第一作者：陈英杰，教授，硕士，从事医学生物学研究。 chenyj2002@foxmail.com
  • 基金资助:

    云南省科委基金资助项目、云南省科技厅-昆明医学院联合专项基金资助项目(98C014Q，2007C0044R，2009CD188)；云南省高校药学重点实验室创新平台建设专项基金(6010902009-2010ZL004);云南省教育厅科学研究基金立项资助项目(2010Y191，07Z10199)。

Interaction between primary cultured human thymocytes and human immunodeficiency virus

Gao Nan-jun1, Chen Ying-jie2, Li Xue-tao3, Kong Wei-yun3, Zhou Yi-ping4   

  1. 1Department of Pediatrics, Yuxi Third People's Hospital, Yuxi  653100, Yunnan Province, China
    2Department of Human Anatomy, Kunming Medical University, Kunming  650031, Yunnan Province, China
    3Department of Orthopedics, Second People’s Hospital of Yunnan Province, Kunming  650051, Yunnan Province, China
    4Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2011-09-06 Revised:2011-10-09 Online:2011-12-10 Published:2011-12-10
  • Contact: Zhou Yi-ping, Doctor, Associate professor, Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650031, Yunnan Province, China 1401545363@qq.com Correspondence to: Kong Wei-yun, Studying for doctorate, Attending physician, Department of Orthopedics, Second People’s Hospital of Yunnan Province, Kunming 650051, Yunnan Province, China kongweiyunkm128@126.com
  • About author:Gao Nan-jun, Associate chief physician, Department of Pediatrics, Yuxi Third People's Hospital, Yuxi 653100, Yunnan Province, China 761470408@qq.com Chen Ying-jie, Master, Professor, Department of Human Anatomy, Kunming Medical University, Kunming 650031, Yunnan Province, China chenyj2002@foxmail.com Gao Nan-jun and Chen Ying-jie contributed equally to this paper.
  • Supported by:

    the Foundation of Yunnan Provincial Science and Technology Committee, No. 98C014Q*; the Joint Specific Foundation of Yunnan Provincial Science and Technology Committee and Kunming Medical University, No. 2007C0044R*, 2009CD188*; the Innovation Foundation of Yunnan Provincial Universities for Key Pharmaceutical Laboratory, No. 6010902009-2010ZL004*; the Scientific Research Program of Education Bureau of Yunnan Province, No. 2010Y191*, 07Z10199*

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393

被引次数

1

摘要：

背景：人类免疫缺陷病毒能够结合人胸腺的细胞CD4受体，从而导致CD4细胞活性降低。
目的：观察人胚胎胸腺组织原代培养与人类免疫缺陷病毒相互作用关系。
方法：取人胎胸腺组织进行原代细胞培养，取胸腺的细胞与人类免疫缺陷病毒1 ⅢB混合培养设为实验组，设置不加人类免疫缺陷病毒培养的胸腺的细胞为对照组。
结果与结论：透射电镜观察显示，培养40 h，人类免疫缺陷病毒诱导的胸腺的细胞内即可发现大量艾滋病病毒颗粒。MTT法和免疫组织化学染色检测结果显示，培养40 h~22 d，HIV诱导胸腺的细胞吸光度值均显著低于对照组(r=0.9733，P < 0.05)，人类免疫缺陷病毒诱导胸腺的细胞膜上和细胞浆Fas-L阳性表达率均升高(P < 0.05)。说明人类免疫缺陷病毒可致原代培养的人胚胎胸腺的细胞活性降低。

关键词: 人类免疫缺陷病毒, 胸腺, 原代培养, 细胞活性, 组织构建

Abstract:

BACKGROUND: Human immunodeficiency virus binds to CD4 receptors in human thymocytes, and thus causes activity decreasing in CD4 cells.
OBJECTIVE: To explore the relationship between human embryo thymocytes and human immunodeficiency virus.
METHODS: Primary cultured human embryo thymocytes were divided into experiment group and control group. Experimental thymocytes were mixed cultured with human immunodeficiency virus 1 Ⅲ B. Control thymocytes were cultured without human immunodeficiency virus.
RESULTS AND CONCLUSION: According to transmission electron microscopy, massive human immunodeficiency virus particles emerged in thymocytes treated with human immunodeficiency virus at the 40th hour after cultivation. According to MTT assay and immunohistochemical staining, the absorbance in thymocytes treated with human immunodeficiency virus was significantly lower than that in the control thymocytes at the 40th hour to the 2nd day after cultivation (r=0.9733, P < 0.05). The Fas-L positive rate of the experiment thymocytes treated with human immunodeficiency virus increased on cell membranes and in cytoplasm (P < 0.05). These findings indicate that human immunodeficiency virus decreases the cell activity in primary cultured human embryo thymocytes.

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