中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8459-8462.doi: 10.3969/j.issn.1673-8225.2011.45.022

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

自体嗅黏膜移植联合β-七叶皂甙钠干预脊髓损伤大鼠基质金属蛋白酶2、9的表达

程  华，刘曾旭，徐年香，罗小凤，余  庆，刘卉芳，刘德明   

  1. 南昌大学基础医学院解剖学教研室，江西省南昌市  330006
  • 收稿日期:2011-04-26 修回日期:2011-06-16 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 刘曾旭，教授，博士，硕士生导师，南昌大学基础医学院解剖学教研室，江西省南昌市330006 lzx6698@yahoo.com.cn
  • 作者简介:程华★，男，1974年生，江西省鄱阳县人，汉族，2011年南昌大学毕业，硕士，副主任医师，主要从事神经损伤与再生研究。 pychenghua@163.com
  • 基金资助:

    江西省教育厅课题(GJJ09097)，江西省科技支撑项目(2009BSB09500)。

Effects of autologous olfactory mucoma combined with beta-aesine sodium on expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in rats following spinal cord injury 

Cheng Hua, Liu Zeng-xu, Xu Nian-xiang, Luo Xiao-feng, Yu Qing, Liu Hui-fang, Liu De-ming   

  1. Department of Anatomy, Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-04-26 Revised:2011-06-16 Online:2011-11-05 Published:2011-11-05
  • Contact: Liu Zeng-xu, Professor, Doctor, Master’s supervisor, Department of Anatomy, Nanchang University, Nanchang 330006, Jiangxi Province, China lzx6698@yahoo.com.cn
  • About author:Cheng Hua★, Master, Associate chief physician, Department of Anatomy, Nanchang University, Nanchang 330006, Jiangxi Province, China pychenghua@163.com
  • Supported by:

    Jiangxi Education Committee, No. GJJ09097*; Jiangxi Scientific Supporting Item, No. 2009BSB09500*

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摘要：

背景：脊髓损伤后基质金属蛋白酶2、9的过表达与脊髓组织的继发性损伤有关。
目的：观察自体嗅黏膜移植联合β-七叶皂甙钠干预脊髓损伤大鼠脊髓组织内基质金属蛋白酶2、9的表达与动物神经功能的恢复情况。
方法：制作半横断脊髓损伤大鼠模型，随机分为4组，分别于腹腔注射β-七叶皂甙钠或生理盐水，以及损伤处植入自体嗅黏膜。
结果与结论：干预后7，14 d自体嗅黏膜组、β-七叶皂甙钠组、自体嗅黏膜联合β-七叶皂甙钠组BBB评分均高于模型对照组(P < 0.05)，自体嗅黏膜联合β-七叶皂甙钠组BBB评分高于自体嗅黏膜组、β-七叶皂甙钠组 (P < 0.05)。干预后1，3，7，14 d自体嗅黏膜联合β-七叶皂甙钠组脊髓内基质金属蛋白酶2、9阳性细胞数少于模型对照组、自体嗅黏膜组、β-七叶皂甙钠组(P < 0.05)。结果提示自体嗅黏膜移植与β-七叶皂甙钠之间有协同作用，可明显改善神经运动功能恢复情况；此种作用可能与其抑制基质金属蛋白酶2、9过度表达有关。

关键词: 自体嗅黏膜, 脊髓损伤, &beta, -七叶皂甙钠, 基质金属蛋白酶, 大鼠

Abstract:

BACKGROUND: After spinal cord injury, over-expression of metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) contributes to secondary tissue damage of the spinal cord.
OJECTIVE: To study the influence of autologous olfactory mucoma combined with β-aesine sodium on expression of MMP-2, 9 and the recovery of neural function of injured spinal cord in adult rats.
METHODS: In this study, healthy Sprague-Dawley rats with T10 spinal cord hemisection were divided into four groups as follows: control group (A), autologous olfactory mucoma transplantation group (B), β-aesine sodium group (C), combination group (D).
RESULTS AND CONCLUSION: The BBB scales showed that rats in groups B, C and D had more improvement than those in group A at series of phases at 7 and 14 days after operation (P < 0.05), and the BBB score of group D was much higher than that of group B and group C (P < 0.05). The expression of MMP-9 and MMP-2 in injured spinal cord increased significantly at all time points. The number of positive cells in group D was less than that in groups B and C at the corresponding time points (P < 0.05), but there were no statistically significant differences between group B and C (P > 0.05). The method of treating spinal cord injury by autologous olfactory mucosa transplantation combined with β-aesine sodium has good curative effect, and can largely improve hind limbs motor function, which may be related to inhibiting the overexpression of MMP-9 and MMP-2 genes.

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