中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8285-8288.doi: 10.3969/j.issn.1673-8225.2011.44.029

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

三种不同方法体外诱导CD4+T细胞向Th17细胞分化与增殖的效率

尹太郎1，邹宇洁1，杨  菁1，何  凡2，李赛姣1，吴庚香1，李  星1，王雅琴1，罗  金1，李  维1，徐望明1   

  1. 1武汉大学人民医院生殖医学中心，湖北省武汉市    430060
    2华中科技大学同济医学院附属同济医院肾内科，湖北省武汉市  430030
  • 收稿日期:2011-07-25 修回日期:2011-09-13 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 杨菁，博士，教授，主任医师，武汉大学人民医院生殖医学中心，湖北省武汉市 430060 通讯作者：何凡，博士，讲师，华中科技大学同济医学院附属同济医院肾内科，湖北省 武汉市430030
  • 作者简介:尹太郎☆，男，1981年生，湖北省仙桃市人，汉族，武汉大学在读博士，主要从事辅助生殖技术及生殖免疫研究。 reproductive@ 126.com

Comparison of differentiation and proliferation efficiency from CD4+ cells to Th17 cells induced by three different methods

Yin Tai-lang1, Zou Yu-jie1, Yang Jing1, He Fan2, Li Sai-jiao1, Wu Geng-xiang1, Li Xing1, Wang Ya-qin1, Luo Jin1, Li Wei1, Xu Wang-ming1   

  1. 1Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
    2Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
  • Received:2011-07-25 Revised:2011-09-13 Online:2011-10-29 Published:2011-10-29
  • Contact: Yang Jing, Doctor, Professor, Chief physician, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China Correspondence to: He Fan, Doctor, Lecturer, Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
  • About author:Yin Tai-lang☆, Studying for doctorate, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China reproductive@126.com

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摘要：

背景：研究认为Th17细胞参与了许多炎症性疾病的发生，但其体外诱导分化效率尚不明确。
目的：比较采用3种不同方法在体外诱导CD4+T细胞向Th17细胞分化与增殖的效率。
方法：分别采取以下3种方法体外诱导Th17细胞：①给予CD3及CD28抗体刺激，并在培养体系中加入白细胞介素6及转化生长因子β，培养3 d。②在方法①的基础上加入白细胞介素1β及肿瘤坏死因子α，培养3 d。③在方法②的基础上第3天洗去细胞因子，培养2 d；再次给予CD3及CD28抗体刺激，并在培养体系中加入白细胞介素23，培养3 d。
结果与结论：3种方法培养后CD4+T细胞中Th17细胞的比例分别为(8.5±2.8)%，(26.9±4.3)%，(44.3±5.5)%，三组之间差异有显著性意义(P < 0.01)。提示在培养体系中加入白细胞介素1β、肿瘤坏死因子α及白细胞介素23，更有利于增加CD4+T细胞向Th17细胞分化的比例。

关键词: CD4+T细胞, Th17细胞, 诱导, 分化, 白细胞介素1&beta, 肿瘤坏死因子&alpha, 白细胞介素23

Abstract:

BACKGROUND: Th17 cells participate in the occurrence of many inflammatory diseases, but their induced differentiation efficiency remains poorly clear.
OBJECTIVE: To compare the differentiation and proliferation efficiency from CD4+ cells to Th17 cells induced by three different methods.
METHODS: Three methods to induce Th17 cells in vitro are as follows: (1) stimulation with CD3 and CD28 antibodies followed by addition of interleukin (IL)-6 and transforming growth factor-β (TGF-β) in the culture medium and finally culture for 3 days; (2) based on method (1), addition of extra IL-1β and tumor necrosis factor-α (TNF-α) and culture for 3 days; (3) based on method (2), washing out previous cytokines on the 3rd day, culture for 2 days, stimulation with CD3 and CD28 antibodies again, addition of IL-23 and finally culture for 3 days.
RESULTS AND CONCLUSION: The proportion of Th17 cells in CD4+T cells was (8.5±2.8)%, (26.9±4.3)%, and (44.3±5.5)% after induced by three above-mentioned methods, respectively, with significant difference among these three methods (P < 0.01). These findings suggest that addition of IL-1β, TNF-α and IL-23 into the culture medium facilitates the induced differentiation from CD4+ cells to Th17 cells.

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