关键词: 过氧化氢, 调节性T细胞, CD4⁺Foxp3⁺ T细胞, 免疫, 小鼠

Abstract:

BACKGROUND: The naturally formed regulator T cells have been confirmed exert important function and role in maintaining organism’s homeostasis, inhibiting inflammation, prevent against transplantation rejection, inhibiting anti-tumor immune response, and preventing and treating auto-immune diseases.
OBJECTIVE: To investigate the effects of intravenous infusion of hydrogen peroxide (H₂O₂) on CD4⁺CD25⁺Foxo3⁺ regulatory T cells in the BALB/c mice.
METHODS: Twelve male BALB/c mice of specific pathogen free level, 6 weeks old, were randomly divided into two groups, H₂O₂ treatment group and PBS group. The mice in the H₂O₂ treatment group were injected 250 μL PBS with 50 mmol/L H2O2 via tail vein. The mice in the PBS group were injected 250 μL PBS via tail vein. At 2 weeks post injection, all mice were killed to collect peripheral blood, spleen and thymus. Flow cytometry was used to detect the proportion of CD4⁺CD25⁺Foxp3 T cells.
RESULTS AND CONCLUSION: The proportion of CD4⁺Foxp3⁺ T cells and CD4⁺Foxp3⁺/CD4⁺ cells in the peripheral blood and spleen of mice in the H₂O₂ treatment group was significantly higher than that in the PBS group (P < 0.05). These findings suggest that intravenous injection of H₂O₂ can increase the proportion of CD4⁺Foxp3⁺ regulatory T cells in the mouse peripheral blood and spleen.