中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8277-8280.doi: 10.3969/j.issn.1673-8225.2011.44.027

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

绿茶多酚可抑制环孢素A所致的血管舒张作用

高文波，姚许平，姜继光   

  1. 宁波市泌尿肾病医院肾移植科，浙江省宁波市  315100
  • 收稿日期:2011-04-01 修回日期:2011-06-16 出版日期:2011-10-29 发布日期:2011-10-29
  • 作者简介:高文波☆，男，1973年生，山东省潍坊市人，汉族，2006年解放军第二军医大学毕业，博士，副主任医师，主要从事泌尿外科(肾移植)方向的研究。
  • 基金资助:

    宁波市自然科学基金(2007A610064)，项目名称：绿茶多酚抑制环孢素A肾毒性。

Alleviative effects of green tea polyphenols on cyclosporine A-induced inhibition of vasorelaxation

Gao Wen-bo, Yao Xu-ping, Jiang Ji-guang   

  1. Department of Kidney Transplantation, Ningbo Urology and Nephrology Hospital, Ningbo  315100, Zhejiang Province, China
  • Received:2011-04-01 Revised:2011-06-16 Online:2011-10-29 Published:2011-10-29
  • About author:Gao Wen-bo☆, Doctor, Associate chief physician, Department of Kidney Transplantation, Ningbo Urology and Nephrology Hospital, Ningbo 315100, Zhejiang Province, China gwbmdoc@yahoo. com.cn
  • Supported by:

    the Natural Science Foundation of Ningbo City, No. 2007A610064*

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被引次数

1

摘要：

背景：血管舒张在环孢素A所致肾毒性的发生过程中起重要的作用。
目的：观察绿茶多酚对环孢素A抑制血管舒张的缓解作用及其机制。
方法：SD大鼠随机平均分为4组：环孢素A组、对照组、环孢素A+绿茶多酚组和绿茶多酚组。经5周的药物处理之后，测定大鼠血尿素氮和肌酐水平。将各组大鼠的胸主动脉环放置于水浴系统上，以乙酰胆碱诱发血管舒张，并观察L-NAME、吲哚美辛和去内皮细胞对血管舒张的作用。
结果与结论：环孢素A组大鼠血尿素氮和肌酐水平显著高于对照组(P < 0.05)。环孢素A组中，乙酰胆碱诱发的血管舒张的最大反应率显著低于对照组和绿茶多酚组。以L-NAME预处理后，环孢素A组，环孢素A+绿茶多酚组和绿茶多酚组中血管舒张显著低于对照组；以吲哚美辛预处理后，对照组，环孢素A+绿茶多酚组和绿茶多酚组的血管舒张显著高于环孢素A组。环孢素A组血管壁组织中一氧化氮代谢物水平显著低于其他组。提示环孢素A能减少血管壁组织中的一氧化氮水平，引起内皮细胞-依赖性的血管舒张反应发生异常变化。

关键词: 绿茶多酚, 血管舒张, 一氧化氮, 环孢素A, 大鼠

Abstract:

BACKGROUND: Vasorelaxation plays an important role in the occurrence of cyclosporine A (CsA)-induced nephrotoxicity.
OBJECTIVE: To observe the alleviative effects of green tea polyphenols (GTP) on CsA-induced inhibition of vasorelaxation and the underlying mechanisms.
METHODS: Sprague-Dawley rats were randomly and evenly divided into four groups: CsA, control, CsA + GTP, and GTP. After 5 weeks of drug treatment, blood urea nitrogen (BUN) and creatinine (Cre) levels were determined. Then the thoracic aorta rings were mounted on a bath system, and acetylcholine was used to induce vasorelaxation. The effects of L-NAME and indomethacin and the denuded vasorelaxation were evaluated.
RESULTS AND CONCLUSION: The BUN and Cre levels in the CsA group were significantly higher than those in the control group (P < 0.05). The maximal response (Emax%) for acetylcholine-induced vasorelaxation in the CsA group was significantly lower than that in the control and GTP groups. After pretreatment with L-NAME, vasorelaxation was significantly lower in the CsA, CsA+GTP and GTP groups than in the control group. After pretreatment with indomethacin, vasorelaxation was significantly higher in the control, CsA +GTP, and GTP groups than in the CsA group. The level of nitric oxide metabolites in the vascular tissue in the CsA group was significantly lower compared with other groups. The results demonstrated that CsA can decrease nitric oxide levels in vascular tissues and induce abnormal endothelium-dependent vasorelaxation, which is mediated by nitric oxide pathway.

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