中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8259-8262.doi: 10.3969/j.issn.1673-8225.2011.44.023

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

肠造瘘式异基因型大鼠胰十二指肠移植模型的建立

张  力，赵雪云，刘胜春   

  1. 重庆医科大学附属第一医院内分泌乳腺外科，重庆市  400016
  • 收稿日期:2011-06-07 修回日期:2011-08-22 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 刘胜春，教授，重庆医科大学附属第一医院内分泌乳腺外科，重庆市 400016 Liushengchun1968@163.com
  • 作者简介:张力★，男，1983年生，四川省广元市人，重庆医科大学附属第一医院内分泌乳腺外科在读硕士，主要从事胰腺移植基础研究。 Zhangli096@ yahoo.cn
  • 基金资助:

    课题受国家自然科学基金项目(30871295)，国家人事部出国留学人员科研启动基金项目 (人发[2001]33)，重庆市科委科研项目(CSTC,2009BB5065)资助。

Establishment of allogeneic pancreaticoduodenal transplantation models in rats with duodenostomy

Zhang Li, Zhao Xue-yun, Liu Sheng-chun   

  1. Department of Breast Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2011-06-07 Revised:2011-08-22 Online:2011-10-29 Published:2011-10-29
  • Contact: Liu Sheng-chun, Professor, Department of Breast Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China liushengchun1968@163.com
  • About author:Zhang Li★, Studying for master’s degree, Department of Breast Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China Zhangli096@ yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871295*; Scientific Research Starting Foundation for Overseas Chinese Scholars, Ministry of Personnel, China, No. renfa[2001]33*; Scientific Research Program of Ministry of Science and Technology of Chongqing, No. CSTC,2009BB5065*

PDF

462

被引次数

2

摘要：

背景：大鼠胰腺移植模型是进行胰腺移植病理生理及移植免疫研究的主要动物模型，但大鼠胰腺模型存在手术难度大、成功率低等缺点，而采用肠造瘘的大鼠胰腺移植能简化操作过程、提高造模成功率。
目的：通过显微外科技术建立一种简单、稳定的异基因大鼠胰腺移植模型。
方法：将供、受体腹腔动脉端侧吻合，供体门静脉与受体左肾静脉以自制导管行袖套吻合，移植十二指肠近端结扎，远端行腹壁造瘘，建立SD→Wistar的糖尿病大鼠胰腺移植模型。
结果与结论：共完成大鼠胰腺移植手术40例，其中34只大鼠术后血糖正常且存活超过3 d，移植成功率为85%。受体大鼠平均存活(8.97±2.05) d，移植后7~10 d为死亡高峰期，移植物呈急性排斥反应病理改变。该模型操作相对简单、成功率高，能够用于胰腺移植中诸如免疫排斥等方面的研究。

关键词: 胰十二指肠移植, 糖尿病, 动物模型, 大鼠, 肠造瘘

Abstract:

BACKGROUND: At present, rat models of pancreatic transplantation are the major animal models for studying pathophysiology and transplantation immunology, but rat models of pancreas are characterized by difficult operation and low success rate. Rat pancreatic transplantation models using intestinal stoma method can simplify operation procedure and enhance success rate in model establishment.
OBJECTIVE: To improve the surgical techniques to establish a rat model of pancreaticoduodenal transplantation, which can be used for experimental research.
METHODS: Pancreaticoduodenal transplantation was performed between SD rats as donors and Wistar rats as recipents. End-to-side anastomosis was performed for arterial reconstruction between donors’ abdominal aorta and recipients’ abdominal aorta segments, while the donors’ portal vein segment was anastomosed with the recipients’ left renal vein by cuff technique. The proximal part of duodenum of the graft was ligated and a stoma was made on the distal end.
RESULTS AND CONCLUSION: Pancreaticoduodenal transplantation was performed in 40 rats. Out of 40 rats, 34 rats survived more than 3 days with normal blood glucose level. The success rate of transplantation was 84.4%.The average survival time of recipients was (8.97±2.05) days. The peak of death appeared on day 7-10 after transplantation, and the pathological changes of typical acute rejection were observed on day 7. The modified model is easily performed successfully, which provides a useful tool for experiment research on transplantation immunology.

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