中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8255-8258.doi: 10.3969/j.issn.1673-8225.2011.44.022

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

裸鼠皮下种植性肝癌模型的建立

白建华1, 2，李  立2，李晓延2，陈  刚2，朱新锋2   

  1. 1昆明医学院，云南省昆明市 650031
    2昆明市第一人民医院肝胆胰外科，云南省昆明市 650011
  • 收稿日期:2011-05-16 修回日期:2011-06-15 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 李立，博士，昆明市第一人民医院肝胆胰外科，云南省昆明市 650011 ynkmlili@yahoo.com
  • 作者简介:白建华☆，男，1980年生，山西省大同市人，汉族，昆明医学院在读博士，医师，主要从事肝胆胰外科及肝移植研究。 jianhuabai3448@sina.com

Establishment of nude mouse models of subcutaneously implanted hepatocellular carcinoma

Bai Jian-hua1,2, Li Li2, Li Xiao-yan2, Chen Gang2, Zhu Xin-feng2   

  1. 1Kunming Medical College, Kunming   650031, Yunnan Province, China
    2Department of Hepatopancreatobiliary Surgery, Kunming First People’s Hospital, Kunming   650011, Yunnan Province, China
  • Received:2011-05-16 Revised:2011-06-15 Online:2011-10-29 Published:2011-10-29
  • Contact: Li Li, Doctor, Department of Hepatopancreatobiliary Surgery, Kunming First People’s Hospital, Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com
  • About author:Bai Jian-hua☆, Studying for doctorate, Physician, Kunming Medical College, Kunming 650031, Yunnan Province, China; Department of Hepatopancreatobiliary Surgery, Kunming First People’s Hospital, Kunming 650011, Yunnan Province, China jianhuabai3448@ sina.com

PDF

415

被引次数

16

摘要：

背景：目前采用Huh7肝癌细胞在BALB/c裸小鼠皮下种植性成瘤的报道较多，但对其成瘤后稳定性的研究较少。
目的：建立稳定的皮下种植性裸鼠肝癌模型。
方法：取1.5×106个对数期细胞Huh7肝癌细胞，种植于裸鼠皮下。成瘤后从体质量、大体形态，肿瘤生长情况，反转录-聚合酶链式反应，苏木精-伊红染色及免疫组织化学多方面进行稳定性及病理学特征鉴定。
结果与结论：肿瘤形成的潜伏期约12 d，肿瘤成功率为92.8%。种植肿瘤组小鼠体质量较正常对照小鼠明显减轻，肿瘤生长迅速，甲胎蛋白基因及蛋白强阳性表达，细胞分裂明显。表明成功建立了稳定的裸鼠皮下种植性肝癌模型。

关键词: 肝癌模型, Huh7, 裸鼠, 肿瘤, 甲胎蛋白

Abstract:

BACKGROUND: There have been more reports describing subcutaneous implantation of Huh7 hepatocellular carcinoma cells into BALB/c nude mice to induce hepatocellular carcinoma, but little is known about the stability after tumor formation.
OBJECTIVE: To establish nude mouse models of stable subcutaneous hepatocellular carcinoma.
METHODS: 1.5×106 Huh7 hepatocellular carcinoma cells were subcutaneously implanted into nude mice. After tumor formation, hepatocellular carcinoma stability and pathological characterization were performed in terms of body mass, gross anatomy, and tumor growth by reverse transplantation-polymerase chain reaction, hematoxylin-eosin staining, and immunohistochemical staining.
RESULTS AND CONCLUSION: The latency of tumor formation was about 12 days, and success rate of tumor formation was 92.8%. In the tumor group, the body mass of nude mice was less compared with the control group, tumor developed rapidly, α-fetoprotein gene and protein expression was strongly positive, and hepatocellular carcinoma cells divided obviously. These findings suggest successful establishment of nude models of subcutaneously implanted hepatocellular carcinoma.

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