中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8251-8254.doi: 10.3969/j.issn.1673-8225.2011.44.021

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

微囊化甲状旁腺兔-鼠移植

王  东1，翟  博1，刘颖新2，武林枫2   

  1. 1哈尔滨医科大学附属第四医院普通外科，黑龙江省哈尔滨市  150001
    2哈尔滨医科大学附属第一医院普通外科，黑龙江省哈尔滨市  150001
  • 收稿日期:2011-06-21 修回日期:2011-09-19 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 翟博，硕士，主治医师，哈尔滨医科大学附属第四医院普通外科，黑龙江省哈尔滨市 150001 zaibo1999@ yahoo.com.cn
  • 作者简介:王东★，男，1975年生，黑龙江省哈尔滨市人，汉族，2004年哈尔滨医科大学毕业，硕士，主治医师，主要从事普通外科基础与临床的研究。 doctorwd1975@163.com
  • 基金资助:

    黑龙江省自然科学基金(D01-13)；黑龙江省教育厅科学技术研究项目(11551208)；黑龙江省卫生厅科研课题(2010-147)。

Xenotransplantation of microencapsulated rabbit parathyroid tissue into rats

Wang Dong1, Zhai Bo1, Liu Ying-xin2, Wu Lin-feng2   

  1. 1Department of General Surgery, Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    2Department of General Surgery, First Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • Received:2011-06-21 Revised:2011-09-19 Online:2011-10-29 Published:2011-10-29
  • Contact: Zhai Bo, Master, Attending physician, Department of General Surgery, Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China zaibo1999@yahoo. com.cn
  • About author:Wang Dong★, Master, Attending physician, Department of General Surgery, Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China doctorwd1975@163.com
  • Supported by:

    the Natural Science Foundation of Heilongjiang Province, No. D01-13*; Science and Technology Research Program of Education Department of Heilongjiang Province, No. 11551208*; Scientific Research Program of Health Department of Heilongjiang Province, No. 2010-147*

PDF

335

被引次数

1

摘要：

背景：微囊化移植技术已经普遍应用于各种实验，成为解决甲状旁腺移植免疫排斥反应的热点领域，但最佳移植部位及其移植失效机制仍是当前亟待解决的问题。
目的：观察微囊化异种甲状旁腺组织移植对Wistar大鼠甲状旁腺功能低下的治疗作用，并探讨最佳移植部位及其失效机制。
方法：制备甲状旁腺功能低下Wistar大鼠模型，建模4周后随机分为肾包囊组、胸锁乳突肌组，空囊组，肾包囊组、胸锁乳突肌组分别将微囊包裹兔甲状旁腺移入鼠肾包囊和胸锁乳突肌，空囊组仅于肾包囊移植空囊。移植后每隔2周取血测血钙，移植后第16周取移植物进行透射电镜检查。
结果与结论：肾包囊组、胸锁乳突肌组16周内血钙水平差异无显著性意义(P > 0.05)，16~20周胸锁乳突肌组血钙呈下降趋势。胸锁乳突肌组微囊周围有大量纤维包裹。提示海藻酸-钡交联微囊可对甲状旁腺组织起到有效的保护作用，使甲状旁腺组织较长时间存活并发挥正常功能。肾包囊是较佳移植部位。

关键词: 微囊, 甲状旁腺, 组织移植, 移植, 异种

Abstract:

BACKGROUND: Microencapsulation has been widely used in various experimental studies. Microencapsulated parathyroid transplantation has become a hot issue, but the better transplantation site and the mechanism underlying failed transplantation are urgently to be solved.
OBJECTIVE: To investigate the therapeutic effects of xenotransplantation of microencapsulated parathyroid tissue on treatment of hypoparathyroidism in Wistar rats and to determine the optimal transplantation site and the mechanism underlying failed transplantation.
METHODS: Wistar rat models of hypoparathyroidism were developed. At 4 weeks after model establishment, rat models were randomly allocated to four groups: renal adipose capsules, chest sternocleidomastoid muscle, and empty microcapsule control groups. Microcapsuled parathyroid tissues were transplanted into renal adipose capsules and chest sternocleidomastoid muscle in renal adipose capsules group and chest sternocleidomastoid muscle group respectively. Empty microcapsules without parathyroid were transplanted into renal adipose capsules in empty microcapsule group. Total serum calcium was measured every other week. In the 16th week, transplant beds were retrieved and subjected to electron microscopic examination. 
RESULTS AND CONCLUSION: There was no significant difference in serum calcium level between renal adipose capsule group and chest sternocleidomastoid muscle group during 16 weeks (P > 0.05). The serum calcium level in the chest sternocleidomastoid muscle group decreased between 16-20 weeks. There were many fiber tissues surrounding the microencapsules in the chest sternocleidomastoid muscle group. Parathyroid tissues can be protected successfully from Aliginate-Ba capsules and then survive functionally for a long time. The renal adipose capsule is the better transplantation site than chest sternocleidomastoid muscle.

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