中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (43): 8050-8054.doi: 10.3969/j.issn.1673-8225.2011.43.018

• 骨与关节实验造模 experimental modeling of the bone and joint • 上一篇    下一篇

低剂量激素诱导股骨头缺血性坏死模型的建立

刘  伟，赵劲民，苏  伟，李晓峰，秦义武   

  1. 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市  530021
  • 收稿日期:2011-04-09 修回日期:2011-06-10 出版日期:2011-10-22 发布日期:2011-10-22
  • 通讯作者: 赵劲民，教授，博士生导师，广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021 zhaojinmin@126.com
  • 作者简介:刘伟★，男，1986年生，江西省萍乡市人，汉族，广西医科大学在读硕士，主要从事蛋白组学、组织工程学与骨科疾病的基础研究。 jxgxliuwei@163.com
  • 基金资助:

    国家自然科学基金资助项目(30860285)，项目名称：早期激素性股骨头缺血性坏死的蛋白组学动态分析。

Establishment of a femoral head model of low dose steroid-induced osteonecrosis

Liu Wei, Zhao Jin-min, Su Wei, Li Xiao-feng, Qin Yi-wu   

  1. Department of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-09 Revised:2011-06-10 Online:2011-10-22 Published:2011-10-22
  • Contact: Zhao Jin-min, Professor, Doctoral supervisor, Department of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Liu Wei★, Studying for master’s degree, Department of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China jxgxliuwei@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860285*

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301

被引次数

4

摘要：

背景：激素性股骨头缺血性坏死的发病机制尚未明确。
目的：单纯应用低剂量激素作用于新西兰大白兔，建立激素性股骨头缺血性坏死模型并观察造模效果。 
方法：将30只新西兰大白兔随机分为2组。实验组每周经臀肌注射4 mg/kg甲泼尼龙琥珀酸钠，对照组于相同时间点注射等量生理盐水。同时迫使动物直立进食、饮水，以增加股骨头的负重。
结果与结论：肌注甲泼尼龙琥珀酸钠后，白兔出现不同程度的精神萎靡、活动减少、体质量减轻等。给药4周后，兔血清总三酰甘油及低密度脂蛋白增高、高密度脂蛋白降低(P < 0.05)；血清碱性磷酸酶及总胆固醇分别在给药6，8周后明显增高 (P < 0.05)。苏木精-伊红染色显示；给药4周后，兔股骨头骨髓腔内脂肪细胞体积增大，数量增多；给药6周后，空骨陷窝率增大；给药8周后，骨小梁稀疏变细、结构紊乱，软骨区可见死骨形成及部分性坏死和软骨面塌陷。说明单纯注射低剂量激素可以复制出典型的激素性股骨头缺血性坏死模型。

关键词: 骨坏死, 激素, 缺血, 动物模型, 兔

Abstract:

BACKGROUND: With the widespread use of steroid hormone, the incidence of steroid-induced osteonecrosis remained high, but the pathological mechanism of human femoral head avascular necrosis is unclear.
OBJECTIVE: To establish a model of steroid-induced osteonecrosis of femoral head and to discuss the effects of low dose of steroid in New Zealand white rabbits.
METHODS: Thirty rabbits were randomly divided into two groups. The experimental group rabbits received intramuscular injection of 4 mg/kg methylprednisolone sodium succinate; the control group rabbits were injected with the same amount of physiological saline at the same time. The venous blood reticulocytes were extracted for serological testing before and at 4, 6 and 8 weeks after exsangquinations. At 4, 6 and 8 weeks after steroid administration, histopathology of femoral head was performed and these rabbits were euthanized in batch.
RESULTS AND CONCLUSION: During the experimental process, one rabbit in the experimental group died. A total of 29 rabbits were involved in the analysis of results. During experimental course, the experimental group rabbits exhibited depressed consciousness and reduced activities and decreased weight loss (P < 0.05) after steroid administration. During the 4th week after administration, cells in the marrow cavity expanded and multiplied in number, which could be observed through the use of light microscope, and the percentage of fat cell size was higher compared with the control group (P < 0.05). At the 6th week, bone empty lacunae percentage was increased compared with the control group (P < 0.05). At the 8th week, the bone trabeculae of the experimental group were reduced and disordered, and disconnected, bone trabecular area percentage was decreased compared with the control group (P < 0.05), and there were parts of sequestrum and collapse in the area of cartilage. At the 8th week, total cholesterol level in the experimental group was significantly higher than that in the control group (P  < 0.05). At the 4th week, total triglycerides and low density lipoprotein level in the experimental group was significantly higher than that in the control group (P  < 0.05); high density lipoprotein level was significantly higher than that in the control group (P  < 0 .05). At the 6th week, serum level of alkaline phosphate was significantly increased compared with the control group (P  < 0.05). Osteobecrosis of femoral head can be induced by low dose of steroid in rabbits.

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