中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7726-7729.doi: 10.3969/j.issn.1673-8225.2011.41.031

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

辛伐他汀抑制高糖诱导人脐静脉内皮细胞的凋亡

徐茂凤1，李永杰2，王婉丽3   

  1. 1长春医学高等专科学校护理系内外护理教研室，吉林省长春市  130031
    2吉林大学白求恩第一医院体检中心，吉林省长春市  130021
    3吉林省第四人民医院呼吸内科，吉林省长春市  130021 
  • 收稿日期:2011-04-19 修回日期:2011-05-23 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 李永杰，副主任医师，吉林大学白求恩第一医院体检中心，吉林省长春市 130021 liyongjie1963@163.com
  • 作者简介:徐茂凤，女，1963年生，吉林省长春市人，1988年白求恩医科大学毕业，汉族，副教授，主要从事冠心病发病机制等相关方面的研究。 xumaofeng1963@163.com

Simvastatin inhibits the apoptosis of human umbilical vein endothelial cells induced by high glucose

Xu Mao-feng1, Li Yong-jie2, Wang Wan-li3   

  1. 1Teaching and Research Section of Clinical Care, Department of Nursing, Changchun Medical College, Changchun  130031, Jilin Province, China
    2Physical Examination Center of the First Hospital of Jilin University, Changchun  130021, Jilin Province, China
    3Department of Respiratory Medicine, Fourth People’s Hospital of Jilin Province, Changchun   130021, Jilin Province, China
  • Received:2011-04-19 Revised:2011-05-23 Online:2011-10-08 Published:2011-10-08
  • Contact: Li Yong-jie, Associate chief physician, Physical Examination Center of the First Hospital of Jilin University, Changchun 130021, Jilin Province, China liyongjie1963@163.com
  • About author:Xu Mao-feng, Associate professor, Teaching and Research Section of Clinical Care, Department of Nursing, Changchun Medical College, Changchun 130031, Jilin Province, China xumaofeng1963@ 163.com

PDF

372

被引次数

12

摘要：

背景：他汀类药物对血管内皮细胞的凋亡是否有影响目前尚不明确。
目的：探讨辛伐他汀对高糖诱导的人脐静脉内皮细胞凋亡的影响。
方法：用DMEM细胞培养液培养人脐静脉内皮细胞，将细胞分成空白对照组、高糖组和高糖+辛伐他汀组，用四甲基偶氮唑蓝比色法测定人脐静脉内皮细胞的存活率，流式细胞仪和Western blot分别检测细胞早期凋亡率及P53蛋白表达。
结果与结论：高糖组及高糖+辛伐他汀组细胞增殖率较空白对照组明显降低(P < 0.01)，而高糖组细胞增殖率较高糖+辛伐他汀组亦降低(P < 0.01)；高糖组P53蛋白表达量及凋亡率较空白对照组及高糖+辛伐他汀组明显增加(P < 0.01)，高糖+辛伐他汀组P53蛋白表达及凋亡率亦明显高于空白对照组(P< 0.01)。表明高糖可通过促进促凋亡蛋白P53的表达进而促进人脐静脉内皮细胞的凋亡，而辛伐他汀可抑制此作用。

关键词: 人脐静脉内皮细胞, 辛伐他汀, 高糖, 细胞凋亡, P53蛋白

Abstract:

BACKGROUND: Whether statins influence apoptosis of vascular endothelial cells remains poorly understood.
OBJECTIVE: To investigate the effects of simvastatin on the apoptosis of human umbilical vein endothelial cells (hUVECs) induced by high glucose and to explore its potential molecular mechanism.
METHODS: hUVECs were cultured with DMEM and then divided into three groups: blank control, high glucose, high glucose + simvastatin. The survival rates of hUVECs were detected by MTT assay after hUVECs were incubated with 25 mmol/L glucose and 1 μmol/L simvastatin for 24 hours. The early apoptosis rate and P53 protein expression were detected by flow cytometry and western blot analysis, respectively.
RESULTS AND CONCLUSION: The value of MTT was significantly decreased in the high glucose group and high glucose + simvastatin group than in the blank control group (P < 0.01), and the MTT value was significantly decreased in the high glucose group than in the simvastatin group (P < 0.01). P53 protein expression and early apoptosis rate were significantly increased in the blank control group than in the high glucose + simvastatin group (P< 0.01), and P53 protein expression and early apoptosis rate were significantly higher in the high glucose + simvastatin group than in the blank control group (P< 0.01). These results suggest that high glucose promotes the apoptosis of hUVECs by promoting P53 protein expression, while simvastatin inhibits the apoptosis of hUVECs.

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