中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7619-7622.doi: 10.3969/j.issn.1673-8225.2011.41.005

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

京尼平苷对大鼠软骨细胞增殖的影响

谭玉林1，鲍同柱2，柳  琴2，韩  钰3，王艳林3，周纳新2，陈  垦2，鄢  飞2   

  1. 1三峡大学第一临床医学院，湖北省宜昌市 443003
    2宜昌市中心人民医院，湖北省宜昌市 443003
    3三峡大学分子学生物研究所，湖北省宜昌市  443003
  • 收稿日期:2011-03-05 修回日期:2011-03-19 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 鲍同柱，硕士，主任医师，宜昌市中心人民医院，湖北省宜昌市 443003 baotongzhu1963@ 163.com
  • 作者简介:谭玉林★，男，1983年生，湖北省巴东县人，三峡大学在读硕士，主要从事骨关节炎方面的研究。 yltan2008@126.com
  • 基金资助:

    湖北省自然科学基金资助项目(2010CDB10701)

Effect of geniposide on proliferation of rat chondrocytes in vitro

Tan Yu-lin1, Bao Tong-zhu2, Liu Qin2, Han Yu3, Wang Yan-lin3, Zhou Na-xin2, Chen Ken2, Yan Fei2   

  1. 1The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei Province, China
    2Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
    3Institute of Molecular Biology, Three Gorges University, Yichang 443003, Hubei Province, China
  • Received:2011-03-05 Revised:2011-03-19 Online:2011-10-08 Published:2011-10-08
  • Contact: Bao Tong-zhu, Master, Chief physician, Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China baotongzhu1963@ 163.com
  • About author:Tan Yu-lin★, Studying for master’s degree, the First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei Province, China yltan2008@126.com
  • Supported by:

    the Natural Science Foundation of Hubei Province, No. 2010CDB10701*

PDF

362

被引次数

20

摘要：

背景：京尼平苷能够促进韧带成纤维细胞的增殖和胶原的合成，促进组织的修复和韧带损伤的愈合。
目的：观察京尼平苷对大鼠软骨细胞增殖的影响。
方法：采用二次酶消化法获得大鼠关节软骨细胞进行传代培养，并取第4代细胞给予0，25，50，100 mmol/L京尼平苷干预，利用MTT比色、流式细胞仪检测细胞周期等方法检测药物干预后第4代软骨细胞的增殖情况。
结果与结论：体外培养的软骨细胞随着传代次数的增加，细胞形态由原代的多角形逐渐变为长梭形；且随着培养时间的延长及京尼平苷浓度的升高，软骨细胞增殖更为显著(P < 0.01)。

关键词: 京尼平苷, 软骨细胞, 增殖, 大鼠, 培养

Abstract:

BACKGROUND: Studies have shown that geniposide can ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen.
OBJECTIVE: To investigate the effect of geniposide on the proliferation of rat chondrocytes in vitro.
METHODS: The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial. The chondrocytes of passage 4 were interfered with 0, 25, 50, 100 mmol/L geniposide. Chonddrocyte proliferation was detected using methyl thiazolyl tetrazolium and flow cytometry.
RESULTS AND CONCLUSION: The phenotype of chondrocyte was changed from original polygonal shape to fusiform with the increased passage. The proliferation of chondrocytes in the 25, 50,100 mmol/L geniposide groups was respectively higher than that in the control group (P < 0.01). Geniposide can stimulate the proliferation of chondrocytes.

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