中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6859-6862.doi: 10.3969/j.issn.1673-8225.2011.37.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

猪大脑灰质组织提取液促进体外培养大鼠成骨细胞的增殖

夏新雷1，黄钢勇1，马  昕1，宫小康1，金慰芳2，黄煌渊1，姜建元1   

  1. 1 复旦大学附属华山医院骨科，上海市  200040
    2复旦大学附属放射医学研究所骨代谢实验室，上海市  200032
  • 收稿日期:2011-05-05 修回日期:2011-07-21 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 马昕，博士，教授，复旦大学附属华山医院骨科，上海市200040 maxin@sh163.net
  • 作者简介:夏新雷☆，男，1974年生，江苏省江阴市人， 2005年复旦大学医学院毕业，博士，主治医师，主要从事脊柱外科疾病的治疗工作。 cormierhuang@yahoo.com.cn

Porcine brain gray matter tissue extract promotes the proliferation of rat osteoblasts cultured in vitro

Xia Xin-lei1, Huang Gang-yong1, Ma Xin1, Gong Xiao-kang1, Jin Wei-fang2, Huang Huang-yuan1, Jiang Jian-yuan1   

  1. 1Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai  200040, China
    2Laboratory of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai   200032, China
  • Received:2011-05-05 Revised:2011-07-21 Online:2011-09-10 Published:2011-09-10
  • Contact: Ma Xin, Doctor, Professor, Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, China maxin@sh163.net
  • About author:Xia Xin-lei☆, Doctor, Attending physician, Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, China cormierhuang@ yahoo.com.cn

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摘要：

背景：有研究表明大鼠脑组织提取液能够促进大鼠成骨细胞和骨髓基质干细胞的增殖。
目的：观察跨种属脑组织固有成分对体外培养大鼠成骨细胞增殖的影响。
方法：提取猪和大鼠大脑灰质、白质、垂体、肢体肌肉组织，分别制成匀浆提取液，并设空白对照组，各组提取液分别用于培养大鼠成骨细胞。比较各种组织提取液对成骨细胞增殖的影响。
结果与结论：与空白对照相比，猪和大鼠的大脑灰质、白质和垂体组织提取液均显著促进了成骨细胞的增殖(P < 0.05)，各组间比较显示猪和大鼠的大脑灰质较白质、垂体和肌肉组织提取液促进成骨细胞增殖的能力更加显著(P < 0.05)。结果证实，猪脑组织提取液能跨种属促进大鼠成骨细胞的增殖，其中以大脑灰质的促进作用最为显著。

关键词: 成骨细胞, 增殖, 大脑灰质, 白质, 垂体, 肌肉, 组织构建

Abstract:

BACKGROUND: Evidence exists that rat brain tissue extract can promote the proliferation of rat osteoblasts and bone marrow stromal stem cells.
OBJECTIVE: To investigate the effects of porcine brain inherent component extract on proliferation of rat osteoblasts cultured in vitro.
METHODS: Porcine and rat brain gray matter, white matter, pituitary gland, limb skeletal muscle were harvested to prepare into homogenate. A blank control group was set. Extract from each tissue was used to culture rat osteoblasts. Effects of each tissue extract on osteoblasts were compared.
RESULTS AND CONCLUSION: Compared with blank control group, porcine and rat brain gray matter, white matter, and pituitary gland all significantly promoted the proliferation of osteoblasts (P < 0.05). Porcine and rat brain gray matter extract better promoted the proliferation of osteoblasts than white matter and pituitary gland extract (P < 0.05). Results showed that porcine brain tissue extract can promote the proliferation of rat osteoblasts, and porcine brain gray matter produced the most significant promoting effects.

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