中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6767-6771.doi: 10.3969/j.issn.1673-8225.2011.36.027

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

羌活鱼研粉物、水提及酸提物含药血清对骨髓间充质干细胞增殖及向成骨分化的影响

谢兴文1，许  伟2，李  宁2   

  1. 1甘肃省中医院，甘肃省兰州市730050
    2甘肃中医学院，甘肃省兰州市  730000
  • 收稿日期:2011-02-03 修回日期:2011-03-15 出版日期:2011-09-03 发布日期:2011-09-03
  • 作者简介:谢兴文☆，男，1972年生，甘肃省甘谷市人，2006年上海中医药大学毕业，博士，副主任医师，硕士生导师，主要从事关节骨科和基础研究。 xxw1972@163.com
  • 基金资助:

    甘肃省自然基金项目(096RJZA040)，课题名称：不同化瘀方法对骨髓间充质干细胞增殖、成骨分化、迁移影响的实验研究。

Effects of Bing’s giant salamander containing serum on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Xie Xing-wen1, Xu Wei2, Li Ning2   

  1. 1Chinese Medicine Hospital in Gansu Province, Lanzhou  730050, Gansu Province, China
    2Gansu College of Traditional Chinese Medicine, Lanzhou  730000, Gansu Province, China
  • Received:2011-02-03 Revised:2011-03-15 Online:2011-09-03 Published:2011-09-03
  • About author:Xie Xing-wen☆, Doctor, Associate chief physician, Master’s supervisor, Chinese Medicine Hospital in Gansu Province, Lanzhou 730050, Gansu Province, China xxw1972@163.com
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 096RJZA040*

PDF

416

被引次数

12

摘要：

背景：羌活鱼经大量的临床观察和报道有促进骨折愈合的作用，其具体的作用机制及其有效成分的研究报道尚少。
目的：分析羌活鱼研粉物、水提及酸提物含药血清对体外培养骨髓间充质干细胞增殖与成骨性分化的影响。
方法：制备羌活鱼研粉、水提物、酸提物，分别采用高、中、低剂量灌服大鼠制得羌活鱼研粉物、水提及酸提物含药血清，以灌服等体积生理盐水制得对照血清；采用贴壁筛选法培养大鼠骨髓间充质干细胞，通过MTT法检测细胞增殖；于干预后的不同时间点分别测定细胞碱性磷酸酶活性、钙盐沉积量、成骨性分化基因表达以及钙化结节数、并比较各组间差异。
结果与结论：羌活鱼研粉物及水提物含药血清具有刺激骨髓间充质干细胞增殖活性及促进其成骨性分化的能力，其中尤其以研粉物中剂量组最为明显，而酸提物含药血清并无此功效。主要表现在细胞增殖、碱性磷酸酶活性、钙盐沉积量、成骨性分化基因和钙化结节数显著高于空白对照组(P < 0.05)。

关键词: 羌活鱼, 血清, 骨髓间充质干细胞, 增殖, 成骨

Abstract:

BACKGROUND: Bing’s giant salamander can promote fracture healing, but the mechanism and active ingredients are rarely reported.
OBJECTIVE: To investigate the effect of Bing’s giant salamander containing serum on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs).
METHODS: Rats were given Bing’s giant salamander by high, moderate and low doses and their serum were obtained after 7 days. The control serum was obtained by giving equal volume physiological saline. Rat BMSCs were obtained from Wistar rats and screened by the adhesive method. The serum was supplemented into the culture medium of rat BMSCs by different concentration. The proliferation of rat BMSCs was analyzed by MTT assay. The osteogenic differentiation markers including Osterix expression, alkaline phosphatase (ALP) activity, calcium deposition and mineralized bone modulus were compared among the exposed groups and the control at different time point.
RESULTS AND CONCLUSION: Moderate Bing’s giant salamander containing serum showed a stronger stimulation of cell proliferation and significantly enhanced the osteogenic differentiation of rat MSCs, indicated by significantly improving ALP activity, calcium deposition, OSX expression and the number of mineralized bone nodules compared to the control and other groups (P < 0.05).

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