中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6772-6775.doi: 10.3969/j.issn.1673-8225.2011.36.028

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

止颤汤干预神经干细胞移植帕金森病大鼠脑内多巴胺及其代谢产物的变化

施慧芬1，路  煜1，宋  杰2，李文涛1   

  1. 1上海市中医医院神经内科，上海市 200071
    2上海中医药大学，上海市 201203
  • 收稿日期:2011-01-04 修回日期:2011-05-29 出版日期:2011-09-03 发布日期:2011-09-03
  • 作者简介:施慧芬，女，1961年生，上海市人，汉族，1984年上海中医药大学毕业，主任医师，主要从事中西医结合神经内科的临床与实验研究。 shf733@163.com
  • 基金资助:

    国家自然基金项目(30772870)，课题的名称：止颤汤对帕金森病模型大鼠细胞移植治疗中神经干细胞分化诱导作用的研究。

Effects of Zhichan Decoction on intracerebral dopamine and metabolites after neural stem cells transplanted in Parkinson disease rats

Shi Hui-fen1, Lu Yu1, Song Jie2, Li Wen-tao1   

  1. 1Department of Neurology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai  200071, China
    2Shanghai University of Traditional Chinese Medicine, Shanghai  201203, China
  • Received:2011-01-04 Revised:2011-05-29 Online:2011-09-03 Published:2011-09-03
  • About author:Shi Hui-fen, Chief physician, Department of Neurology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China shf733@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772870*

PDF

406

被引次数

29

摘要：

背景：如何促进脑内多巴胺含量的增加以及减少多巴胺的代谢，是治疗帕金森病的热点所在。
目的：从多巴胺代谢途径角度观察止颤汤对神经干细胞移植帕金森病大鼠的脑黑质中多巴胺及其代谢产物含量的变化。
方法：以大鼠脑立体定位和1-甲基-4-苯基-1，2，3，6-四氢吡啶建立帕金森病大鼠模型。应用高效液相色谱法测定帕金森病大鼠中脑多巴胺及其代谢产物的含量。
结果与结论：止颤汤可以提高神经干细胞移植后帕金森病大鼠中脑多巴胺及其代谢产物双羟苯乙酸的含量，但对代谢产物高香草酸无明显影响。通过促进帕金森病大鼠干细胞移植后神经干细胞的存活，使之定向分化为多巴胺能神经元并分泌多巴胺，同时抑制多巴胺分解达到治疗作用。

关键词: 帕金森病, 止颤汤, 干细胞移植, 多巴胺代谢, 大鼠模型

Abstract:

BACKGROUND: How to make the transplanted neural stem cells differentiated into more dopaminergic neurons, so as to accomplish the plerosis of injured dopaminergic neuron. This problem solving will be the breakthrough progress for the treatment of Parkinson disease (PD).
OBJECTIVE: To study the effects of Zhichan Decoction on substantia nigra dopamine (DA) and metabolites, and the survival and directional differentiation after neural stem cells were transplanted from metabolic pathway in PD rats.
METHODS: PD rats models were established by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine. The contents of DA, homovanillic acid (HVA) and 3, 4-dihydroxy phenyl acetic acid (DOPAC) were measured with high-performance liquid chromatography (HPLC). 
RESULTS AND CONCLUSION: Zhichan Decoction could increase the content of DA and DOPAC. But there were no effects on HVA. The Zhichan Decoction has the effect to promote the neural stem cells differentiating into dopaminergic neurons in PD rats; At the same time, it could inhibit DA breaks down, so that the therapeutic effect was achieved.

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