中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6355-6359.doi: 10.3969/j.issn.1673-8225.2011.34.021

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

脱细胞羊膜预防生物衍生肌腱修复鸡屈趾肌腱Ⅱ区缺损修复后的粘连

郝  鹏1，项  舟2，罗静聪2，杨志明2   

  1. 1四川省医学科学院，四川省人民医院骨科，四川省成都市 610072
    2四川大学华西医院干细胞与组织工程研究室，四川省成都市 610041
  • 收稿日期:2011-03-08 修回日期:2011-04-28 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 郝鹏☆，男，1977年生，四川省成都市人，汉族，2007年四川大学毕业，博士学位，主治医师，主要从事骨关节损伤与功能重建研究。 hhaopeng@163.com
  • 作者简介:郝鹏☆，男，1977年生，四川省成都市人，汉族，2007年四川大学毕业，博士学位，主治医师，主要从事骨关节损伤与功能重建研究。 hhaopeng@163.com

Effects of humnan acellular amniotic membrane on preventing tendon adhesion after repairing digital flexor tendons by derived tendon in chickens

Hao Peng1, Xiang Zhou2, Luo Jing-cong2, Yang Zhi-ming2   

  1. 1Department of Orthopedic Surgery, Sichuan Provincial Hospital, Chengdu  610072, Sichuan Province, China
    2Division of Stem Cells and Tissue Engineering, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-03-08 Revised:2011-04-28 Online:2011-08-20 Published:2011-08-20
  • Contact: Hao Peng☆, Doctor, Attending physician, Department of Orthopedic Surgery, Sichuan Provincial Hospital, Chengdu 610072, Sichuan Province, China hhaopeng@163.com
  • About author:Hao Peng☆, Doctor, Attending physician, Department of Orthopedic Surgery, Sichuan Provincial Hospital, Chengdu 610072, Sichuan Province, China hhaopeng@163.com

PDF

459

被引次数

28

摘要：

背景：羊膜有预防肌腱修复后粘连的作用，但存在一定的免疫排斥反应。
目的：探讨脱细胞羊膜对生物衍生肌腱修复鸡屈趾深肌腱Ⅱ区缺损修复后肌腱粘连的预防作用。
方法：分别以罗曼鸡右爪的第2，3，4趾将实验分成生物衍生肌腱组，生物衍生肌腱+脱细胞羊膜组以及自体肌腱组，建立屈趾深肌腱Ⅱ区1 cm缺损模型。以生物衍生肌腱桥接第2，3趾缺损，以自体肌腱桥接第4趾的肌腱缺损，在第3趾的生物衍生肌腱和上下吻合口周围包裹完整的脱细胞羊膜。
结果与结论：生物衍生肌腱组与自体肌腱组移植物为外源性愈合，生物衍生肌腱+脱细胞羊膜组羊膜有效地防止了成纤维细胞向修复区域内浸润，形成内源性愈合。肌腱粘连程度生物衍生肌腱+脱细胞羊膜组优于生物衍生肌腱组和自体肌腱组，生物衍生肌腱组和自体肌腱组相似，移植物周围的炎症反应生物衍生肌腱+脱细胞羊膜组>生物衍生肌腱组>自体肌腱组。证实脱细胞羊膜能通过机械性阻挡周围肉芽组织向修复区域内生长而防止外源性愈合造成的肌腱粘连。

关键词: 脱细胞羊膜, 生物衍生肌腱, 粘连, 肌腱缺损, 腱鞘

Abstract:

BACKGROUND: Amnion can depress the degree of tendon adhesion, but also can induce the immune reaction.
OBJECTIVE: To evaluate the effects of human acellular amniotic membrane (HAAM) on Roman chickens tendon adhesion when the derived tendon materials were used to repair the flexor digitorum profundus tendon defects.
METHODS: The flexor digitorum profundus tendons of three fingers of each claw in 40 Roman chickens were resected and 1 cm defect was made as experimental model. The defects were repaired by three methods respectively (group A: derived tendon materials; group B: derived tendon materials encapsulated by HAAM; group C: autograft).
RESULTS AND CONCLUSION: In group A and C, the tendon grafts were healed exogenously. In group B, the fibroblast infiltration was stopped by HAAM efficiently and the tendon grafts healed endogenously. The degree of adhesion: group B was better than group A and group C, group A was similar with group C. The inflammatory reactions around grafts were group B＞group A＞group C. HAAM lessened the tendon adhesion by mechanically preventing the exogenous healing caused by granulation tissue infiltrating the anastomotic stoma and would be a good biological material in preventing postoperative adhesions of flexor tendon.

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