中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (3): 416-420.doi: 10.3969/j.issn.2095-4344.0553

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

保留黄韧带预防模型兔腰椎椎板切除后硬脊膜粘连

李树文,杨  哲,银和平,吴一民,白  明,杜志才,王宇鹏,孟格栋   

  1. (内蒙古医科大学第二附属医院, 内蒙古自治区呼和浩特市  010030)
  • 收稿日期:2018-01-16 出版日期:2019-01-28 发布日期:2019-01-28
  • 通讯作者: 银和平,硕士研究生,主任医师,教授,内蒙古医科大学第二附属医院, 内蒙古自治区呼和浩特市 010030
  • 作者简介:李树文,男,1974年生,内蒙古自治区呼和浩特市人,汉族,2016年南方医科大学毕业,博士,主任医师,主要从事脊柱退变性疾病研究。 并列第一作者:杨哲,男,1990年生,山西省长治市人,汉族,内蒙古医科大学第二附属医院研究生,主要从事脊柱退变性疾病研究。
  • 基金资助:

    内蒙古自治区自然科学基金(2017MS08126),项目负责人:李树文

Preserving ligamentum flavum for preventing dural adhesions after lumbar surgery in model rabbits

Li Shuwen, Yang Zhe, Yin Heping, Wu Yimin, Bai Ming, Du Zhicai, Wang Yupeng, Meng Gedong   

  1.  (the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China)
  • Received:2018-01-16 Online:2019-01-28 Published:2019-01-28
  • Contact: Yin Heping, Master candidate, Chief physician, Professor, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Li Shuwen, MD, Chief physician, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Yang Zhe, Master candidate, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Li Shuwen and Yang Zhe contributed equally to this work.
  • Supported by:

    the Natural Science Foundation of Inner Mongolia Region, No. 2017MS08126 (to LSW)

摘要:

文章快速阅读:

文题释义:
黄韧带:是附着于脊椎上位椎板下缘的腹侧面,下位椎板上缘的后面及上缘,向外侧黄韧带附着部延伸至小关节突关节囊的富含弹性纤维蛋白的韧带组织。正常情况下黄韧带呈紧张状态,除具有维持脊柱稳定性的功能外,还可保护硬膜外脂肪,进而保持神经和硬脊膜囊在一定范围内的活动性。
腰椎椎板切除后硬脊膜粘连:腰椎外科手椎板切除后继发粘连是椎板切除后效果不佳或出现新问题的重要原因之一。防止椎板切除后粘连的措施与材料很多,但均不理想,黄韧带为自体组织,可作为尝试作为椎板切除后防粘连材料。
                                 
摘要
背景
:瘢痕组织的生成以及随之形成的与硬脊膜及神经根的粘连一直是影响椎板切除后疗效的重要因素之一。如何减少或预防这种粘连或瘢痕形成,是临床医生着重研究的问题。
目的:探索术中保留黄韧带对预防腰椎椎板切除后硬脊膜周围粘连的作用。
方法:将40只新西兰大白兔随机分为黄韧带切除组与黄韧带保留组,每组实验动物20只,均行L4-5椎板切除术,模拟髓核摘除,术中分别保留或切除黄韧带。参考Rydell-balazs评分标准和Nuss-baum组织学评分标准,评定椎板切除后硬脊膜与周围结组粘连情况。
结果与结论:①所有手术均顺利完成,黄韧带保留组椎板切除区仍可见瘢痕组织,硬脊膜与周围组织粘连轻,容易钝性分离。黄韧带切除组可见手术创面肌肉明显瘢痕化,并有类骨质形成,其与脊柱背侧肌肉及软组织粘连紧密,钝性分离非常困难;②黄韧带保留组Rydell-balazs与Nuss-baum评分显著低于和黄韧带切除组(P < 0.05);③结果提示,保留黄韧带可降低硬膜外瘢痕形成,减少硬脊膜与周围组织粘连。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0001-6607-7156(银和平)

关键词: 黄韧带, 硬脊膜, 粘连, 瘢痕, 椎板, 硬膜外脂肪, 神经根, Rydell-balazs评分, Nuss-baum评分, 组织构建

Abstract:

BACKGROUND: Formation of scar tissue and the consequent formation of adhesions with dura and nerve root is an important factor for postoperative outcomes of laminectomy. How to reduce or prevent the adhesion or scar formation has been paid much attention in the clinic. 
OBJECTIVE: To explore the effect of preserving the ligamentum flavum on preventing dural adhesions after laminectomy in lumbar surgery.
METHODS: Forty New Zealand white rabbits were randomly divided into ligamentum flavum resection group and ligamentum flavum preservation group (n=20 per group). All animals underwent L4-5 laminectomy, simulating nucleus pulpotomy. Postoperative dural adhesion was assessed according to Rydell-balazs and Nuss-baum criteria.
RESULTS AND CONCLUSION: The operations were successfully completed, and there was visible scar in the operation area in the ligamentum flavum preservation group, the dura adhesion was easy to be separated bluntly from surrounding tissue. In the ligamentum flavum resection group, the scar tissue around the dura was very dense and the dural adhesions were serious, even osteoid was observed. The Rydell-balazs and Nuss-baum scores in the ligamentum flavum preservation group were significantly lower than those in the ligamentum flavum resection group (P < 0.05). To conclude, retaining the ligamentum flavum can reduce epidural scarring and adhesion of the dura to the surrounding tissue.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words:  Tissue Adhesions, Ligamentum Flavum, Lumbar Vertebrae, Tissue Engineering

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