中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5827-5830.doi: 10.3969/j.issn.1673-8225.2011.31.030

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

L-谷氨酰胺与肝脏大部分切除后的再生

安  军，付维利，丁  洁   

  1. 大连市友谊医院腹部肿瘤外科，辽宁省大连市   116001
  • 收稿日期:2011-03-03 修回日期:2011-04-12 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 安军☆，男，1967年生，辽宁省大连市人，汉族，2010年日本爱知医科大学毕业，博士，副主任医师，主要从事肿瘤外科及普外临床及科学研究。anjun1976@126.com
  • 作者简介:安军☆，男，1967年生，辽宁省大连市人，汉族，2010年日本爱知医科大学毕业，博士，副主任医师，主要从事肿瘤外科及普外临床及科学研究。anjun1976@126.com

Effects of orally supplied L-glutamine on hepatic regeneration in partial hepatectomic rats

An Jun, Fu Wei-li, Ding Jie   

  1. Department of Abdominal Oncology, Dalian Municipal Friendship Hospital, Dalian 116001, Liaoning Province, China
  • Received:2011-03-03 Revised:2011-04-12 Online:2011-07-30 Published:2011-07-30
  • Contact: An Jun☆, Doctor, Associate chief physician, Department of Abdominal Oncology, Dalian Municipal Friendship Hospital, Dalian 116001, Liaoning Province, China anjun1976@126.com
  • About author:An Jun☆, Doctor, Associate chief physician, Department of Abdominal Oncology, Dalian Municipal Friendship Hospital, Dalian 116001, Liaoning Province, China anjun1976@126.com

PDF

334

被引次数

5

摘要：

背景：L-谷氨酰胺作为DNA和谷胱甘肽等合成的氮前体，在肝组织再生，肝细胞增殖的过程中扮演着极其重要的角色。
目的：观察经饮食由来补充L-谷氨酰胺对大鼠肝脏大部切除后肝再生能力的影响。
方法：Wistar大鼠随机分组3组，L-谷氨酰胺组和L-丙氨酸组大鼠肝切除前分别灌服10% L-谷氨酰胺或10%L-丙氨酸，肝切除后继续加入饮用水中饮用，对照组肝切除前后均使用饮用水。
结果与结论：大鼠肝切除后72 h L-谷氨酰胺组肝再生率明显高于对照组及L-丙氨酸组(P < 0.05)。肝切除后24 h和72 h L-谷氨酰胺组肝细胞增殖均明显高于对照组和L-丙氨酸组(P < 0.01；P < 0.05)。肝切除后24 h和72 h总RNA水平在两种氨基酸与对照组之间差异无显著性意义。肝切除后72 h基因组DNA的含量L-谷氨酰胺组显著高于对照组和L-丙氨酸组 (P < 0.05)。提示肝损伤围手术期投用高浓度L-谷氨酰胺对大鼠肝再生有促进作用，而投用L-丙氨酸则没有此作用。

关键词: 肝脏大部分切除, L-谷氨酰胺, 肝再生, 增殖细胞核抗原, 大鼠

Abstract:

BACKGROUND: As a necessary nitrogen provider in the processes of DNA and glutathione (GSH) biosynthesis, also a nitrogen source of plasma ammonia formation, L-glutamine is considered to play important roles in hepatic cell proliferation. But the functions and the mechanisms of L-glutamine in liver regeneration are not clearly studied and still need further investigation.
OBJECTIVE: To investigate the effects of dietary supplied L-glutamine on hepatic regeneration in a rat model of partial hepatectomy.
METHODS: Wistar rats were randomly divided into three groups. Before partial hepatectomy, rats in the L-glutamine and L-alanine groups were orally administered 10% L-glutamine and 10% L-alanine, respectively. After partial hepatectomy, rats from these two groups were given water containing 10% L-glutamine and 10% L-alanine, respectively. The control group rats were allowed to drink water before and after partial hepatectomy.
RESULTS AND CONCLUSION: At 72 hours after partial hepatectomy, restituted liver mass was significantly greater in the L-glutamine group than in the control and L-alanine groups (P < 0.05). At 24 and 72 hours after partial hepatectomy, hepatocyte proliferation was more obvious in the L-glutamine group than in the control and L-alanine groups (P < 0.01; P < 0.05). At 24 and 72 hours after partial hepatectomy, there was no significant difference in total RNA level between L-glutamine and L-alanine groups and control groups. At 72 hours after partial hepatectomy, genomic DNA content was significantly greater in the L-glutamine group than in the control and L-alanine groups (P < 0.05). These results showed that application of high concentration L-glutamine in the perioperative stage of liver injury promotes hepatic regeneration in rats after partial hepatectomy, but L-alanine does not produce this effect.

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