中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4619-4622.doi: 10.3969/j.issn.1673-8225.2011.25.015

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

改性纳米羟基磷灰石/PLGA材料同骨髓基质干细胞复合后相关生物学评价

谭羽莹1，张  舵1，李玉新2，孙  颖2，徐  洋3，李  春1，金洪娟1，邵  英1   

  1. 1吉林大学第一医院整形美容外科，吉林省长春市  130021
    2东北师范大学药物基因和蛋白筛选国家工程实验室，吉林省长春市  130024
    3厦门大学附属第一医院思明分院整形美容外科，福建省厦门市  361002
  • 收稿日期:2011-01-04 修回日期:2011-04-15 出版日期:2011-06-18 发布日期:2014-01-10
  • 通讯作者: 邵英，博士，副教授，吉林大学第一医院整形美容外科，吉林省长春市 130021 leleshao@hotmail.com
  • 作者简介:谭羽莹★，女，1985年生，吉林省长春市人，汉族，吉林大学第一医院在读硕士，主要从事整形美容外科及组织工程方面的研究。 tyy_316@163.com
  • 基金资助:

    吉林大学科学前沿与交叉学科创新项目(200903068)。

Relative biological evaluation of hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly(lactide-co-glycolde) cultured with bone marrow mesenchymal stem cells

Tan Yu-ying1, Zhang Duo1, Li Yu-xin2, Sun Ying2, Xu Yang3, Li Chun1, Jin Hong-juan1, Shao Ying1   

  1. 1Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun  130021, Jilin Province, China
    2National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun  130024, Jilin Province, China
    3Department of Plastic and Reconstructive Surgery, Siming Branch of First Affiliated Hospital, Xiamen University, Xiamen  361002, Fujian Province, China
  • Received:2011-01-04 Revised:2011-04-15 Online:2011-06-18 Published:2014-01-10
  • Contact: Shao Ying, Doctor, Associate professor, Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China lele_shao@163.com
  • About author:Tan Yu-ying★, Studying for master’s degree, Department of Plastic and Reconstructive Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China tyy_316@163.com
  • Supported by:

    Science Frontiers and Cross-disciplinary Innovation Projects of Jilin University, No. 200903068*

PDF

350

被引次数

6

摘要：

背景：改性纳米羟基磷灰石/聚乙交酯-丙交酯复合材料(L-lactic acid oligomer/Poly(lactide-co-glycolde)，PLGA/g-HA)因具有良好的稳定性及机械性能，目前备受关注。
目的：观察骨髓基质干细胞和改性PLGA/g-HA体外复合后的细胞活性及生物相容性。
方法：原代培养兔骨髓基质干细胞，传代培养至第3代，MTT比色法检测在不同浓度PLGA/g-HA浸提液(10%、30%、50%、80%)中骨髓基质干细胞的增殖情况，以及骨髓基质干细胞在PLGA/g-HA表面的黏附性及其细胞形态。
结果与结论：于培养后1，3 d测得在不同浓度浸提液下骨髓基质干细胞的A值，10%浸提液组和对照组相比无显著性差异，4种浓度浸提液的细胞毒性均为1级；扫描电镜观察到骨髓基质干细胞在PLGA/g-HA表面逐渐伸展，形成伪足，最终牢固锚定在材料表面。提示在PLGA/g-HA的浸提液中骨髓基质干细胞能够发生增殖，对细胞无毒性。骨髓基质干细胞可以黏附在PLGA/g-HA表面且形态正常，生长状态良好，证明PLGA/g-HA具有良好的相容性和黏附性，可作为修复骨缺损的复合组织工程骨。

关键词: 羟基磷灰石, PLGA, 骨髓基质干细胞, 组织工程支架, 生物活性, 生物相容性

Abstract:

BACKGROUND: The hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly (lactide-co-glycolde) (PLGA/g-HA) composite material is preferable to good stability and favorable mechanical property. As scaffold cultured with bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To study the bioactivity and biocompatibility of BMSCs cultured with PLGA/g-HA composite material in vitro.
METHODS: Third passage BMSCs of rabbits were cultured in PLGA/g-HA extract at different concentrations (10%, 30%, 50% and 80%), and cell proliferation was detected by MTT method. Meanwhile, the cell adhesion and morphology were observed with scanning electron microscopy.
RESULTS AND CONCLUSION: There was no significant difference between 10% group and negative group at 1 and 3 days after culture. The cytotoxic grade was Grade 1 in all the four concentrations. BMSCs could stretch pseudopodium gradually and adherent on the surface of PLGA/g-HA firmly. The results indicated that: ①BMSCs could proliferate in PLGA/g-HA leaching liquor. ②BMSCs have a good state on the surface of PLGA/g-HA. And we can apply the scaffold to bone defect repair, because of its good biocompatibility and cellular adhesion.

中图分类号: