中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4615-4618.doi: 10.3969/j.issn.1673-8225.2011.25.014

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

复合壳聚糖纳米微球聚乳酸-羟基乙酸/纳米羟基磷灰石缓释载体系统对蛋白的缓释作用

安世昌，孙  健，李亚莉，许尧详，王  科，陈立强，肖文林   

  1. 青岛大学医学院附属医院口腔颌面外科，山东省青岛市  266021
  • 收稿日期:2010-11-18 修回日期:2010-12-18 出版日期:2011-06-18 发布日期:2014-01-10
  • 通讯作者: 孙健，博士，教授，主任医师，硕士生导师，青岛大学医学院附属医院口腔颌面外科，山东省青岛市 260021 sunjianqy@126.com
  • 作者简介:安世昌★，男，1984年生，陕西省西安市人，汉族，青岛大学医学院在读硕士，主要从事口腔颌面外科学研究。 anshichang510@163.com

Delayed release of compound chitosan nano-microspheres PLGA/nHA control-releasing carrier systems

An Shi-chang, Sun Jian, Li Ya-li, Xu Yao-xiang, Wang Ke, Chen Li-qiang, Xiao Wen-lin   

  1. Departocent of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical School, Qingdao  266021, Shandong Province, China
  • Received:2010-11-18 Revised:2010-12-18 Online:2011-06-18 Published:2014-01-10
  • Contact: Sun Jian, Doctor, Professor, Chief physician, Master’s supervisor, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical School, Qingdao 266021, Shandong Province, China sunjianqy@126.com
  • About author:An Shi-chang★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University Medical School, Qingdao 266021, Shandong Province, China anshichang510@163.com

PDF

528

被引次数

14

摘要：

背景：聚乳酸-羟基乙酸支架材料具有良好的生物相容性、无毒、可以良好的塑性，并具有一定的强度和韧性。但其降解产物为酸性，会影响局部pH值变化，不利组织生长。
目的：制备能够良好缓释蛋白类药物的复合支架。
方法：以牛血清蛋白为模型药物，以离子凝胶法制备壳聚糖微球。将微球与纳米羟基磷灰石和聚乳酸-羟基乙酸按一定比例混合，以冰粒子为致孔剂，采用粒子沥虑-冷冻干燥复合工艺制备CMs/nHA/PLGA复合缓释支架。利用扫描电镜、透射电镜、压泵仪和力学性能测试仪检测复合支架的形态和性能，并考察其在体外对蛋白类药物释放的规律。
结果与结论：制备的壳聚糖纳米微球形态良好，呈规则球形或类球形，粒径分布在220~770 nm，以380~650 nm为多。微球对药物的载药量为39.2%，包封率为68.3%，两者均与牛血清蛋白的初始量相关，载药量随牛血清蛋白初始量的增加而增加，包封率则反之。复合支架呈白色多孔状，孔径为125~355 mm，孔与孔之间联通良好，孔隙率达83.4%，压缩强度为1.4~ 2.1 MPa，10周降解率为28.6%。PLGA/nHA支架对牛血清蛋白的2 d累积释放量为85%，而壳聚糖和CMs/nHA/PLGA复合支架对牛血清蛋白的9 d累积释放量分别是为48.9%和35.7%。提示制作的壳聚糖纳米微球和CMs/nHA/PLGA支架材料对牛血清蛋白有良好的缓释作用，复合支架材料形态好，强度和降解速率合适。

关键词: 复合支架, 壳聚糖, 聚乳酸-羟基乙酸, 缓释载体, 骨组织工程

Abstract:

BACKGROUND: Polylactic acid-glycolic acid (PLGA) is a kind of biodegradable polymer with excellent biocompatibility, plasticity and certain strength and toughness. But its degradation products are acidic, which can affect local pH and inhibit tissue growth.
OBJECTIVE: To prepare composite scaffolds with excellent characteristics of delayed release of proteins.
METHODS: Using bovine serum album (BSA) as model protein, chitosan microspheres (CMs) were prepared by ionotropic gelation. Ice particulates were used as porogen. Composite scaffolds were prepared with CMs/nHA/PLGA by freeze-drying. Scanning electron microscope, transmission electron microscope, mercury porosimeter and universal testing machine ware used to observe the characteristic and morphology of the composite scaffolds.
RESULTS AND CONCLUSION: The CMs were spherical in shape with a regular surface. The diameters of the CMs were in the range of 220-770 nm, mainly ranging 380-650 nm. The encapsulation efficiency of the CMs was 68.3%, and the loading capacity was 39.2%. The both were related with the initial concentration of BSA. The pore diameter of the composite scaffolds was about 125-355 m, the porosity was 83.4%, and the compressive strength was about 1.4-2.1 MPa. The cumulative degradation was 28.6% at 10 weeks. The cumulative release of BSA from PLGA/nHA scaffolds was 85% in 2 days, which from CMs and CMs/nHA/PLGA scaffolds was 48.9% and 35.7% in 9 days. The CMs microspheres and CMs/nHA/PLGA scaffolds have a desirable release rates for BSA and strength, are expected to use as carrier for growth factor and bone tissue engineering scaffolds.

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