中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (12): 1856-1862.doi: 10.12307/2023.102

• 组织工程骨材料 tissue-engineered bone • 上一篇    下一篇

聚三亚甲基碳酸酯/β-磷酸三钙微球支架修复大鼠骨缺损

黄燕妮1，杨  华2，杨东梅1，胡旭麟3，高  鸿4，黄毅娜1   

  1. 1四川大学华西公共卫生学院/四川大学华西第四医院，四川省成都市  610041；2四川大学华西公共卫生学院分析测试中心，四川省成都市610041；3中国科学院成都有机化学研究所，四川省成都市  610041；4四川大学轻工科学与工程学院，四川省成都市  610065
  • 收稿日期:2021-09-22 接受日期:2021-11-11 出版日期:2023-04-28 发布日期:2022-07-30
  • 通讯作者: 黄毅娜，教授，四川大学华西公共卫生学院/四川大学华西第四医院，四川省成都市 610041
  • 作者简介:黄燕妮，女，1997年生，四川省德阳市人，汉族，四川大学华西公共卫生学院在读硕士，主要从事食品毒理学研究。

Rat bone defect repaired with polytrimethylene carbonate/beta-tricalcium phosphate microsphere scaffold

Huang Yanni1, Yang Hua2, Yang Dongmei1, Hu Xulin3, Gao Hong4, Huang Yina1   

  1. 1West China School of Public Health, Sichuan University/West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; 2Analysis and Assay Center, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan Province, China; 3Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, Sichuan Province, China; 4College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China
  • Received:2021-09-22 Accepted:2021-11-11 Online:2023-04-28 Published:2022-07-30
  • Contact: Huang Yina, Professor, West China School of Public Health, Sichuan University/West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Huang Yanni, Master candidate, West China School of Public Health, Sichuan University/West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

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摘要：

文题释义：
聚三亚甲基碳酸酯：是一种生物相容性高、可吸收和降解的聚合物，可用甲基丙烯酸酯端基官能化，以在立体平版印刷术中实现光交联。由于聚三亚甲基碳酸酯通过酶介导的表面侵蚀缓慢降解，机械性能随着时间的推移保持良好，降解产物也具有良好的再吸收性和耐受性。
β-磷酸三钙：为无机材料中的代表材料，具有良好的生物相容性、降解性、骨诱导性，是一种经典的骨缺损填充材料，但该材料降解速率快和易脆断的特性，使其难以应用到骨骼承重处的重建。

背景：目前单一支架材料难以满足骨组织工程的成骨需要，多种材料组合的微球支架可以扬长避短，具有巨大的应用潜力。
目的：探究新型复合材料聚三亚甲基碳酸酯/β-磷酸三钙微球支架在动物体内的生物相容性、降解性及骨诱导性。
方法：制备β-磷酸三钙骨水泥与聚三亚甲基碳酸酯/β-磷酸三钙微球支架。将18只雄性SD大鼠按体质量分层随机分为3组，空白对照组双侧股骨缺损处不植入任何材料，对照组双侧股骨缺损处植入β-磷酸三钙骨水泥，实验组双侧股骨缺损处植入聚三亚甲基碳酸酯/β-磷酸三钙微球支架，每组6只。术后4，8，12周，取出双侧股骨进行苏木精-伊红染色、Masson染色及骨钙素免疫组化染色。
结果与结论：①苏木精-伊红染色与Masson染色：术后4周时，空白对照组骨缺损处由纤维组织包绕，大量炎性细胞聚集；对照组材料大部分降解，由纤维组织包绕，材料边缘有少量新生骨基质、骨小梁形成，周围有少量炎性细胞；实验组材料无明显降解，由纤维组织包绕，材料边缘有少量新生骨基质、骨小梁形成，周围有少量炎性细胞。至术后12周时，空白对照组骨小梁形态规则，彼此连接，无炎性细胞；对照组材料完全降解，骨小梁形态规则，形成厚的板层状，无炎性细胞；实验组材料部分降解，骨质增多、致密，无炎性细胞。②免疫组化染色：随着时间的延长，对照组骨缺损处骨钙素表达呈先升高后降低的趋势，空白对照组、实验组骨钙素表达呈逐渐升高趋势，对照组、实验组各时间点的骨钙素表达均高于空白对照组(P < 0.05)，实验组术后12周的骨钙素表达高于对照组(P < 0.05)。③结果表明：聚三亚甲基碳酸酯/β-磷酸三钙微球支架具有较好的生物相容性、骨诱导性和适中的生物降解性。

https://orcid.org/0000-0002-5505-0414 (黄燕妮) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 骨组织工程, 微球支架, 聚三亚甲基碳酸酯, β-磷酸三钙, 生物相容性, 降解性, 骨诱导性, 骨钙素

Abstract: BACKGROUND: At present, it is difficult for a single scaffold material to meet the needs of osteogenesis in bone tissue engineering. Microsphere scaffolds combined with a variety of materials can maximize their strengths and avoid their weaknesses, which has great application potential.
OBJECTIVE: To explore the biocompatibility, degradability, and osteoinductivity of a new type of polytrimethylene carbonate/β-tricalcium phosphate in animals. 
METHODS: The β-tricalcium phosphate bone cement and polytrimethylene carbonate/β-tricalcium phosphate microsphere scaffold were prepared. Eighteen male SD rats were randomly divided into three groups (n=6) according to their weight. No material was implanted in the bilateral femoral defects in the blank control group. The β-tricalcium phosphate bone cement was implanted in the bilateral femoral defects in the control group. Polytrimethylene carbonate/β-tricalcium phosphate microsphere scaffold was implanted at bilateral femoral defects in the experimental group. At 4, 8, and 12 weeks after operation, the bilateral femurs were taken out for hematoxylin-eosin staining, Masson staining, and immunohistochemical staining for osteocalcin. 
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining and Masson staining: At 4 weeks after operation, the bone defect in the blank control group was surrounded by fibrous tissue and numerous inflammatory cells gathered. In the control group, the material was mostly degraded and surrounded by fibrous tissue, with a small amount of new bone matrix and bone trabecular formation at the edge of the material, which was surrounded by a few inflammatory cells. The material in the experimental group did not degrade remarkably and was surrounded by fibrous tissue. Some new bone matrix and bone trabecular formation were detected at the edge of the material, which was surrounded by a few inflammatory cells. At 12 weeks postoperatively, the blank control group presented regular trabecular bone, which was connected to each other, without inflammatory cells. In the control group, the material was completely degraded and trabecular bone was regular, forming a thick lamellar shape, without inflammatory cells. In the experimental group, materials were partially degraded; bone mass was increased and dense, with the absence of inflammatory cells. (2) Immunohistochemical staining: With prolonged time, the expression of osteocalcin in the bone defect of the control group increased first and then decreased. The expression of osteocalcin in the blank control group and the experimental group showed a gradually increasing trend. The expression of osteocalcin at each time point was higher in the control and experimental groups than that of the blank control group (P < 0.05). The expression of osteocalcin in the experimental group was higher than that of the control group at 12 weeks postoperatively (P < 0.05). (3) These findings exhibit that the polytrimethylene carbonate/β-tricalcium phosphate microsphere scaffold has good biocompatibility, osteoinductivity, and moderate biodegradability. 

Key words: bone tissue engineering, microsphere scaffolds, polytrimethylene carbonate, β-tricalcium phosphate, biocompatibility, degradability, osteoinductivity, osteocalcin

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