中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4607-4610.doi: 10.3969/j.issn.1673-8225.2011.25.012

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

许旺细胞在不同孔径丝素蛋白支架上的生长

焦  微1，赵  霞2，陆  艳2，汪  洋3，文建川4，卲正中4   

  1. 复旦大学附属华山医院，1麻醉科，2耳鼻咽喉头颈外科，上海市  200040；3复旦大学上海医学院细胞与组织胚胎学，上海市  200032；4复旦大学高分子系，上海市  200433
  • 收稿日期:2010-11-09 修回日期:2010-12-09 出版日期:2011-06-18 发布日期:2014-01-10
  • 通讯作者: 赵霞，教授，复旦大学附属华山医院耳鼻咽喉头颈外科，上海市200040
  • 作者简介:焦微★，女，1984年生，山西省晋城市人，汉族，2009年复旦大学上海医学院毕业，硕士，医师，主要从事组织工程学研究。
  • 基金资助:

    国家自然科学基金资(30772400)项目，丝素导管的理化性质对面神经再生影响的研究。上海医学院青年骨干科研启动基金 09L-12 ,不同形貌丝素材料对雪旺细胞生长影响的相关性研究(B类)。

Growth of Schwann cells in silk fibroin scaffolds with different pore sizes

Jiao Wei1, Zhao Xia2, Lu Yan2, Wang Yang3, Wen Jian-chuan4, Shao Zheng-zhong4   

  1. 1Department of Anesthesiology, 2Department of Otolaryngology Head and Neck Surgery, Huashan Hospital, Affiliated to Fudan University, Shanghai  200040, China; 3Department of Cell and Embryology, Shanghai Medical College of Fudan University, Shanghai  200032, China; 4Department of Macromolecule, Fudan University, Shanghai  200433, China
  • Received:2010-11-09 Revised:2010-12-09 Online:2011-06-18 Published:2014-01-10
  • Contact: Zhao Xia, Professor, Department of Otolaryngology Head and Neck Surgery, Huashan Hospital, Affiliated to Fudan University, Shanghai 200040, China zhaoxiaabc@126.com
  • About author:Jiao Wei★, Master, Physician, Department of Anesthesiology, Huashan Hospital, Affiliated to Fudan University, Shanghai 200040, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30772400*; Priming Scientific Research Foundation for the Young Backbones in Shanghai Medical College, Fudan University, No. 09L-12*

PDF

458

被引次数

3

摘要：

背景：细胞在生物支架上的生长行为受到支架表面形貌、润湿性、孔径及孔隙率等多种因素影响。
目的：观察许旺细胞在不同孔径丝素蛋白支架上的生长情况。
方法：制备大孔径50~60 µm、小孔径10~   20 µm两种多孔丝素材料。选用许旺细胞永生化细胞R3 [33-10ras3]为种子细胞，当细胞在培养瓶底形成致密单层时即可消化细胞并进行接种实验，将许旺细胞悬液种于不同形貌的多孔丝素材料表面。复合培养1周后，扫描电镜观察许旺细胞的生长形态及增殖等情况。
结果与结论：不同孔径丝素材料的表面可见许旺细胞生长情况不一。在10~20 µm孔径材料支架上，细胞浓度较低，细胞表现为特异的双极性形态，细胞与细胞之间或平行排列，或首尾相连成细胞链；细胞与细胞之间或平行排列，或首尾相连成细胞链；在50~ 60 µm孔径丝素材料支架上，细胞浓度较高，细胞多为球形，单个分散在多孔支架表面，或呈现成团成串葡萄样聚集在孔的底部，未延展成双极性形态，只有极少量生长在孔与孔之间嵴上的细胞呈双极样。说明多孔丝素蛋白支架的孔径对许旺细胞的黏附、生长有一定的影响，许旺细胞更适合生长在孔径略大于胞体直径的支架材料上。

关键词: 丝素蛋白, 多孔支架, 许旺细胞, 组织工程, 表面形貌

Abstract:

BACKGROUND: The growth behaviors of cells on the biomaterials scaffold may be affected by the topography, pore size, wettability, porosity and other factors.
OBJECTIVE: This research is aimed to observe the growth and proliferation of Schwann cells in silk fibroin scaffolds with different pore sizes.
METHODS: Two kinds of silk fibroin scaffolds with different pore sizes were prepared, including a large pore size scaffold (pore size 50-60 μm) and a small pore size scaffold (pore size 10-20 μm). Schwann cells (R3 [33-10ras3]) served as seed cells and incubated in 37 ℃, 5% CO2 incubation box. When cells filled up the culture bottle bottom and formed a dense monolayer, they were digested and the cell concentration adjusted, then Schwann cells were seeded onto the surface of the porous silk fibroin scaffolds with different pore sizes. After seven days of co-culture, the growth and proliferation of Schwann cells were observed under scanning electron microscope.
RESULTS AND CONCLUSION: The growth of Schwann cells on the surface of silk fibroin scaffolds with different pore sizes was varied. On the surface of small pore size scaffold (10-20 µm), the cell density was low, while the phenotype of cells was bipolar, cells arranged in parallel or linked as the cell chains. On the surface of large pore size scaffold (50-60 µm), more cells could be seen, but most of the cells were in the shape of single sphere, cells clustered on the surface of the porous scaffold or aggregated as a bunch of grape at the bottom of pores. Only few cells were bipolar and lied on the ridge between the pores. The result showed that the pore size of porous silk fibroin scaffolds is an influential factor for the growth and adhesion of Schwann cells. Schwann cells are conducive to grow on the scaffolds with pore size larger than cell body diameter.

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