中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (12): 2179-2182.doi: 10.3969/j.issn.1673-8225.2011.12.022

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米纤维介导的紫杉醇/阿霉素序贯联合治疗SHg-44胶质瘤

王占峰1，2，孙玉芹2，韩海玲1，徐秀玲3，景遐斌3   

  1. 1北华大学附属医院脑外2科，吉林省吉林市   132011
    2吉林大学，吉林省长春市 130011
    3中国科学院长春应用化学研究所，吉林省长春市 130011
  • 收稿日期:2010-09-11 修回日期:2011-01-17 出版日期:2011-03-19 发布日期:2011-03-19
  • 作者简介:王占峰☆，男，1978年生，吉林省桦甸市人，汉族，2008年吉林大学毕业，博士，副教授，主要从事脑肿瘤和脑血管病的临床和基础研究。 1206hhl@sina.com
  • 基金资助:

    国家自然科学基金资助项目(20904002)；吉林省科技厅杰出青年科研基金项目(20090193)；吉林省教育厅“十一五”重大科学技术攻关(2009139)；吉林市杰出青年科研基金项目(20090606)。

Nano-fiber-mediated paclitaxel combined with doxorubicin for sequential chemotherapy of SHg-44 glioma

Wang Zhan-feng1, 2, Sun Yu-qin1, Han Hai-ling1, Xu Xiu-ling3, Jing Xia-bin3   

  1. 1Second Department of Brain Surgery, Affiliated Hospital of North China University, Jilin 132011, Jilin Province, China
    2Jilin University, Changchun  132011, Jilin Province, China
    3Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun  130022, Jilin Province, China
  • Received:2010-09-11 Revised:2011-01-17 Online:2011-03-19 Published:2011-03-19
  • About author:Wang Zhan-feng☆, Doctor, Associate professor, Second Department of Brain Surgery, Affiliated Hospital of North China University, Jilin 132011, Jilin Province, China 1206hhl@sina.com
  • Supported by:

    National Natural Science Foundation of China, No. 20904002*; Scientific Research Foundation for Distinguished Young Scientists of Technology Bureau of Jilin Province, No. 20090193*; the "Eleventh Five-Year" Major Scientific and Technological Research of Education Department of Jilin Province, No. 2009139*; Scientific Research Foundation for Distinguished Young Scientists of Jilin, No. 20090606*

PDF

459

被引次数

11

摘要：

背景：纳米纤维技术可同时担载多种药物，避开血脑屏障限制实现脑胶质瘤的序贯联合化疗。
目的：用乳液电纺法制备同时担载紫杉醇和阿霉素的聚乙二醇-聚乳酸共聚物纳米纤维并实现两种药物的序贯释放，探讨纳米纤维介导的紫杉醇和阿霉素序贯联合治疗SHg-44胶质瘤的效果及机制。
方法:实验分为4组，1640培养液对照组，1%阿霉素组，1%紫杉醇组，5%(阿霉素+紫杉醇)组。采用高效液相色谱法测定紫杉醇和阿霉素的体外释放情况。四甲基偶氮唑盐法检测纳米纤维介导的紫杉醇和阿霉素序贯治疗对SHg-44胶质瘤细胞的增殖抑制率；流式细胞仪检测法检测紫杉醇和阿霉素序贯治疗对SHg-44胶质瘤细胞的凋亡诱导作用。
结果与结论：纳米纤维介导的紫杉醇和阿霉素序贯治疗对SHg-44胶质瘤细胞具有明显的生长抑制及促凋亡作用,且作用效果好于单独药物应用。提示聚乙二醇-聚乳酸纳米纤维作为一种药物载体能提高紫杉醇和阿霉素对SHg-44胶质瘤细胞增殖抑制和诱导凋亡作用。

关键词: 胶质瘤, 紫杉醇, 阿霉素, 序贯化疗, 细胞凋亡

Abstract:

BACKGROUND: Nano-fiber technology can simultaneously support many kinds of drugs, avoid the limitation of blood-brain barrier and achieve sequential combination chemotherapy of glioma.
OBJECTIVE: By means of emulsion-electrospinning method, both paclitaxel (PTX) and doxorubicin (DOX), were successfully loaded into poly (ethylene glycol)-b-poly (L-lactic acid) copolymer (PEG-PLA) nanofiber to realize sequential chemotherapy and to explore the effect and mechanism of SHg-44 glioma mediated by PEG-PLA nano-fiber.
METHODS: The experiment was divided into 4 groups: 1640 culture medium group, 1% DOX group, 1% PTX group, and 5% (DOX + PTX) group. The release in vitro of PTX and DOX was measured by high performance liquid chromatography (HPLC). The inhibition ratio of SHg-44 glioma cell proliferation on nano-fiber mediated PTX and DOX sequential chemotherapy was determined by MTT assay. Flow cytometry was used to detect the SHg-44 glioma cell apoptosis induced by PTX and DOX sequential chemotherapy.
RESULTS AND CONCLUSION: Nano-fiber mediated PTX and DOX sequential chemotherapy had significant growth inhibition and pro-apoptotic effect to SHg-44 glioma cell, and the effect was better than individual drug application. It is indicated that PEG-PLA nano-fiber as a drug carrier can increase the effect of PTX and DOX on SHg-44 glioma cell proliferation and induction of apoptosis.

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