中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1210-1214.doi: 10.3969/j.issn.1673-8225.2011.07.015

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

天然、重组水蛭素对随意皮瓣淤血模型血管内皮细胞生长因子的影响

郭应信，殷国前，李佳荃，肖  汉   

  1. 广西医科大学第一附属医院整形美容外科，广西壮族自治区南宁市 530021
  • 收稿日期:2010-07-28 修回日期:2010-08-27 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 殷国前，主任医师，博士导师，广西医科大学第一附属医院整形美容外科，广西壮族自治区南宁市 530021 yingq61@163. com
  • 作者简介:郭应信☆，男，1971年生，广西壮族自治区桂平市人，汉族，广西医科大学在读博士，主治医师，主要从事组织修复、血管瘤方面的研究。 nngyx_2008@ 126.com
  • 基金资助:

    广西自然科学基金项目(2010GXNSFA013141)，天然、重组水蛭素对随意皮瓣淤血模型大鼠中VEGF、CD34的影响。

Effect of natural and recombinant hirudin on vascular endothelial cell growth factor in random skin flap congestion models

Guo Ying-xin, Yin Guo-qian, Li Jia-quan, Xiao Han   

  1. Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-07-28 Revised:2010-08-27 Online:2011-02-12 Published:2011-02-12
  • Contact: Yin Guo-qian, Chief physician, Doctoral supervisor, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China yingq61@163.com
  • About author:Guo Ying-xin☆, Studying for doctorate, Attending physician, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China nngyx_2008@126. com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2010GXNSFA013141*

PDF

627

被引次数

32

摘要：

背景：随意皮瓣移植后易发生皮瓣静脉淤血，甚至坏死，临床与动物实验证明水蛭素能够提高淤血皮瓣的成活率。
目的：观察水蛭素对大鼠随意皮瓣淤血模型成活保护作用。
方法：Wistar大鼠30只背部设计随意皮瓣10 cm×3 cm制成淤血模型，随机分为天然水蛭素组、重组水蛭素组和对照组，分别于皮下注射5 U天然水蛭素、5 U重组水蛭素和生理盐水。术后测定血管内皮细胞生长因子含量及血管内皮细胞生长因子mRNA表达量，计算皮瓣成活率。
结果与结论：天然水蛭素组与重组水蛭素组皮瓣成活率比对照组高(P< 0.05)。天然水蛭素组与重组水蛭素组比对照组新生毛细血管血管内皮细胞生长因子表达多(P < 0.05)，且天然水蛭素组最多。提示天然、重组水蛭素均能够促进随意皮瓣血管内皮细胞生长因子表达，有利于新生血管的生成，能够提高随意皮瓣的成活率，且天然水蛭素效果优于重组水蛭素。

关键词: 天然水蛭素, 重组水蛭素, 血管内皮细胞生长因子, 随意皮瓣, 皮瓣存活率

Abstract:

BACKGROUND: Flap venous congestion, even necrosis, occurs after random skin flap transplantation. Clinical and animal experiments have proved that hirudin can improve the survival rate of congestion flap.
OBJECTIVE: To investigate the protective effect of hirudin on random skin flap congestion.
METHODS: Thirty Wistar rats were established animal model of venous congestion. Dorsal random skin flap was prepared on each animal with 10 cm × 3 cm in size. The models were randomly divided into the natural hirudin, recombinant hirudin and control groups, which received subcutaneous injection of 5 U natural hirudin, recombinant hirudin or physiological saline, respectively. Vascular endothelial cell growth factor (VEGF) levels and VEGF mRNA expression were measured after surgery. The flap survival rate was calculated.
RESULTS AND CONCLUSION: Compared with the control group, the flap survival rate of the natural hirudin or recombinant hirudin group was higher (P < 0.05). The VEGF expression of the natural hirudin or recombinant hirudin group was greater than that of the control group (P < 0.05), especially greatest in the natural hirudin group. The results demonstrated that, both natural and recombinant hirudin can promote VEGF expression in random flaps, accelerate angiogenesis and increase survival rate of random flaps. The natural hirudin shows superior effects to recombinant hirudin.

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