中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (5): 861-864.doi: 10.3969/j.issn.1673-8225.2011.05.024

• 移植与中医药 transplantation and traditional Chinese medicine • 上一篇    下一篇

雷公藤甲素PG490-88联合环孢素A免疫抑制诱导肾移植急性排斥反应模型大鼠的免疫耐受

戴  澄，王  平，张新涛，黄志恒，朱  量   

  1. 深圳大学第一医院暨深圳市第二人民医院肾移植科，广东省深圳市  518035
  • 收稿日期:2010-10-05 修回日期:2010-12-31 出版日期:2011-01-29 发布日期:2011-01-29
  • 作者简介:戴澄★，男，1961年生，江苏省镇江市人，汉族，1997年暨南大学医学院研究生班毕业，硕士，副主任医师，副教授，从事肾脏移植，肾脏外科基础和临床研究。 Hxming2007@yahoo.cn
  • 基金资助:

    深圳市科技计划项目基金(200903038) 。

Triptolide PG490-88 combined with ciclosporin A induces immune tolerance in a rat renal allograft rejection model

Dai Cheng, Wang Ping, Zhang Xin-tao, Huang Zhi-heng, Zhu Liang   

  1. Department of Kidney Transplantation, Shenzhen Second People’s Hospital, First Hospital of Shenzhen University, Shenzhen   518035, Guangdong Province, China
  • Received:2010-10-05 Revised:2010-12-31 Online:2011-01-29 Published:2011-01-29
  • About author:Dai Cheng★, Master, Associate chief physician, Associate professor, Department of Kidney Transplantation, Shenzhen Second People’s Hospital, First Hospital of Shenzhen University, Shenzhen 518035, Guangdong Province, China Hxming2007@yahoo.cn
  • Supported by:

    the Science and Technology Plan of Shenzhen, No. 200903038*

PDF

430

被引次数

20

摘要：

背景：近年来的研究发现雷公藤甲素具有很好的抗排异、抗肿瘤作用。PG490-88是雷公藤甲素的提取物，能够预防移植物抗宿主病和诱导免疫耐受。
目的：探讨雷公藤甲素PG490-88联合环孢素A在大鼠肾移植急性排斥反应中诱导免疫耐受的作用。
方法：改良法建立大鼠肾移植动物模型。40只Wistar大鼠做供体、40只SD大鼠做受体，随机数字表法均分为4组：对照组给予常规饮食。雷公藤甲素组给予雷公藤甲素PG490-88 20 mg/(kg•d)灌胃。环孢素A组给予环孢素A 15 mg/(kg•d)灌胃；联合治疗组：PG490-88 20 mg/(kg•d)+环孢素A 15 mg/(kg•d)灌胃。分别检测肾移植后各组1，3，5，7，14 d外周血白细胞介素2受体水平和肾移植后大鼠的脾脏淋巴细胞组织中的白细胞介素2活性蛋白表达。
结果与结论：3个治疗组的白细胞介素2活性和白细胞介素2受体均明显低于对照组(P < 0.05)。其中，联合治疗组低于雷公藤甲素组和环孢素A组，差异有显著性意义(P < 0.05)。提示，雷公藤甲素PG490-88通过对白细胞介素2、白细胞介素2受体的影响，来达到免疫抑制作用，不但具有免疫抑制作用，而且还能够诱导一定程度的免疫耐受，联合环孢素A效果会更好。

关键词: 雷公藤甲素, 肾移植, 免疫抑制, 排斥反应, 抗排异

Abstract:

BACKGROUND: Studies demonstrated that triptolide has good effect on anti-rejection and anti-tumor. PG490-88 is extracted from triptolide, which can prevent graft versus host disease and induce immune tolerance.
OBJECTIVE: To investigate the effect of PG490-88 combined with ciclosporin A on inducing immune tolerance in a rat renal allograft rejection model.
METHODS: Kidney transplantation models were constructed using improved method. Forty Wistar rats served as donors, and 40 SD rats served as recipients. All rats were randomly divided into 4 groups. Rats were regular raised in the control group. Rats were lavaged with triptolide PG490-88 [20 mg/(kg•d)], ciclosporin A [15 mg/(kg•d)] or PG490-88 [20 mg/(kg•d)]+ ciclosporin A
[15 mg/(kg•d)] in the PG490-88, ciclosporin A or the combination group. Serum levels of interleukin 2-receptor (IL-2R) and activity of IL-2 in spleen lymph cells were detected respectively at 1, 3, 5, 7 and 14 days after transplantation．
RESULTS AND CONCLUSION: Serum levels of IL-2R and activity of IL-2 were detected lower in 3 treated groups than those in the control group (P < 0.05). Among the 3 treated groups, the combination group was lower than that of the PG490-88 and ciclosporin A groups (P < 0.05). Triptolide PG490-88 performs its function of immunosuppressant on the liver transplantation through IL-2 and IL-2R. It is not only has immunosuppressive action, but also can induce immune tolerance, the effects would be better when combined with ciclosporin A.

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