中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 290-293.doi: 10.3969/j.issn.1673-8225.2011.02.024

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

大鼠骨骼肌细胞异柠檬酸脱氢酶和琥珀酸脱氢酶活性在不同训练负荷时的变化

张  敏1，陈立军1，周  蔚2   

  1. 武警医学院，1生物化学教研室，2研究生管理大队，天津市  300162
  • 收稿日期:2010-11-06 修回日期:2010-12-03 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 陈立军，教授，硕士生导师，武警医学院生物化学教研室，天津市 300162 chenlijun67@ eyou.com
  • 作者简介:张敏★，女，1978年生，山东省济南市人，汉族，2007年天津医科大学毕业，硕士，讲师，主要从事生物化学教学与研究工作。 zhangmin53421@163.com
  • 基金资助:

    武警医学院重点科研项目(WKH2009Z02)，课题名称：不同训练负荷条件下身体机能的代谢评定及机制探讨。

Effects of exercise training modes at different work intensities on activity of isocitrate dehydrogenase and succinic dehydrogenase in rat skeletal muscles  

Zhang Min1, Chen Li-jun1, Zhou Wei2   

  1. 1Department of Biochemistry, 2Graduate Management Brigade, Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
  • Received:2010-11-06 Revised:2010-12-03 Online:2011-01-08 Published:2011-01-08
  • Contact: Chen Li-jun, Professor, Master’s supervisor, Department of Biochemistry, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China chenlijun67@eyou. com
  • About author:Zhang Min★, Master, Lecturer, Department of Biochemistry, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China zhangmin53421@ 163.com
  • Supported by:

    the Key Project of Medical College of Chinese People’s Armed Police Force, No. WKH2009Z02

PDF

423

被引次数

12

摘要：

背景：异柠檬酸脱氢酶和琥珀酸脱氢酶是糖有氧氧化过程中的关键酶，不同训练方式和训练时间对骨骼肌异柠檬酸脱氢酶和琥珀酸脱氢酶活性影响的报道较少。
目的：探讨不同训练负荷条件对大鼠骨骼肌异柠檬酸脱氢酶和琥珀酸脱氢酶活性的影响。
方法：参照BEDFORD TG标准，建立大鼠有氧、无氧和有氧无氧交替运动跑台训练模型，正常饲养的大鼠作为对照。训练结束后，紫外分光光度计检测大鼠骨骼肌异柠檬酸脱氢酶和琥珀酸脱氢酶的活性。
结果与结论：有氧运动4，6周，大鼠骨骼肌异柠檬酸脱氢酶和琥珀酸脱氢酶活性均明显升高(P < 0.05)；交替运动2周，异柠檬酸脱氢酶和琥珀酸脱氢酶活性增加(P < 0.05)，运动至4，6周时，两种酶活性均下降(P < 0.05)；而无氧运动对异柠檬酸脱氢酶和琥珀酸脱氢酶活性无影响。结果提示：长期的有氧运动和短时间的有氧无氧交替运动有利于提升骨骼肌异柠檬酸脱氢酶和琥珀酸脱氢酶的活性。

关键词: 训练, 骨骼肌, 异柠檬酸脱氢酶, 琥珀酸脱氢酶, 有氧, 有氧无氧交替

Abstract:

BACKGROUND: Isocitrate dehydrogenase (IDH) and succinic dehydrogenase (SDH) play key role in aerobic oxidation. However, reports about the effects of different training models and training periods on the activity of IDH and SDH in skeletal muscle have little coverage.
OBJECTIVE: To observe the effects of different training on the activity of IDH and SDH in rat skeletal muscles. 
METHODS: According to BEDFORD TG standards, aerobic exercise, anaerobic exercise and aerobic and anaerobic cross-training motion models were established using treadmill running at different work intensities. Rats in control group lived in the cages quietly, and all the animals were killed immediately when the exercise training completed. Then the activity of IDH and SDH were determined by ultraviolet spectrophotometer.
RESULTS AND CONCLUSION: The activity of IDH and SDH were obviously increased at 4 and 6 weeks after treadmill training (P < 0.05). To the cross-training group, they increased after 2 weeks’ training (P < 0.05), however, the activity of IDH and SDH decreased at 4 and 6 weeks after aerobic exercise training (P< 0.05). While to anaerobic exercise group, the activity of IDH and SDH had no significant differences. The present findings demonstrated that, long-time aerobic exercise and short-time aerobic and anaerobic cross-training can protect the activity of IDH and SDH.

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