中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (51): 9546-9548.doi: 10.3969/j.issn.1673-8225.2010.51.010

• 组织工程血管材料 tissue-engineered vascular materials • 上一篇    下一篇

壳聚糖单体对内皮细胞血管化功能的影响　　

许  庆， 陈  静，罗祥林，陈元维，万昌秀   

  1. 四川大学高分子科学与工程学院医用高分子与人工器官系，四川省成都市 610065
  • 出版日期:2010-12-17 发布日期:2010-12-17
  • 通讯作者: 万昌秀，教授，博士生导师，四川大学高分子科学与工程学院医用高分子与人工器官系，四川省成都市 610065 wanchangxiu@163.com 通讯作者：陈元维，硕士生导师，四川大学高分子科学与工程学院医用高分子与人工器官系，四川省成都市 61006
  • 作者简介:许庆★，女，1986年生，山西省运城市人，汉族，四川大学高分子科学与工程学院在读硕士，主要从事生物材料的研究。 xuqing_1985@163.com
  • 基金资助:

    国家自然科学基金面上项目：可降解生物材料与内皮细胞界面反应对内皮细胞功能蛋白表达的影响及机理研究(No.30370411)。

Effects of chitosan monomer on the devascularization function of endothelial cells

Xu Qing, Chen Jing, Luo Xiang-lin, Chen Yuan-wei, Wan Chang-xiu   

  1. Department of Medical Polymers and Artificial Organs, Institute of Polymer Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Wan Chang-xiu, Master, Professor, Doctoral supervisor, Department of Medical Polymers and Artificial Organs, Institute of Polymer Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China wanchangxiu@163.com Correspondence to: Chen Yuan-wei, Master’s supervisor, Department of Medical Polymers and Artificial Organs, Institute of Polymer Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China
  • About author:Xu Qing★, Studying for master’s degree, Department of Medical Polymers and Artificial Organs, Institute of Polymer Science and Engineering, Sichuan University, Chengdu 610065, Sichuan Province, China xuqing_1985@163.com
  • Supported by:

    General Program of the National Natural Science Foundation of China, No. 30370411*

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摘要：

背景：壳聚糖对多种细胞生长的刺激作用可能是诱导血管再生和肉芽生成的原因。但是，并未就壳聚糖在使用过程中产生的降解产物对血管形成的影响进行研究。
目的：研究壳聚糖的水解降解终产物D-氨基葡萄糖对内皮细胞形成血管相关行为(包括增殖、迁移和形成管状结构)的影响。
方法：将不同浓度的D-氨基葡萄糖盐酸盐生理盐水溶液与内皮细胞共培养，以生理盐水作为阴性对照组。用MTT比色法检测细胞增殖活性，计算相对增殖率；照相记录不同时间的划痕情况，计算相对迁移率。观察内皮细胞在胶原表面形成的管状结构，计算管状结构长度的平均值及管状结构相对生长率。
结果与结论：D-氨基葡萄糖浓度从10 nmol/L上升至1 mmol/L时，相对增殖率与对照组比较差异无显著性意义(P > 0.05)。当浓度≥10 mmol/L时，相对增殖率明显降低(P < 0.01)。当浓度≤10 mmol/L时，细胞发生迁移，相对迁移率与对照组比较差异无显著性意义(P > 0.05)。当浓度为100 mmol/L，相对迁移率急剧下降至0.63%。提示，随着浓度升高，相对生长率逐渐降低，但在10 mmol/L时，细胞迁移无显著变化，管状结构生成率突增，促进内皮细胞血管化功能。

关键词: 壳聚糖, D-氨基葡萄糖, 内皮细胞, MTT, 迁移, 管状结构

Abstract:

BACKGROUND: Chitosan stimulation of the growth of various cells may induce angiogenesis and granulation formation. However, little evidence reports the degradation products of chitosan on the angiogenesis.
OBJECTIVE: To investigate the influences of chitosan hydrolytic degradation product D-glucosamine on related behavior of endothelial cells to form blood vessel (including proliferation, migration and the formation of tube-like structure).
METHODS: Endothelial cells were cultured with D-glucosamine physiological saline solution of different concentrations, with saline as negative control. MTT colorimetric assay was used to determine cell proliferation and to calculate the relative growth rate. A nick was made with a scraper and recorded by taking some photos at different time, and cell relative migration rate was calculated. Tubular frame on the collagen surface formed by endothelial cells was observed. The average length was calculated to obtain the relative tubular structure ratio.
RESULTS AND CONCLUSION : When the D-glucosamine concentration rose from 10 nmol/L to 1 mmol/L, the relative growth rate did not change markedly compared with control group (P > 0.05). When the concentration ≥ 10 mmol/L, relative growth rate reduced remarkably (P < 0.01). When the concentration ≤ 10 mmol/L, cells migrated, and relative migration rate did not change markedly compared with control group (P > 0.05). Relative migration rate fell down rapidly to 0.63% at 100 mmol/L. Along with an increasing concentration, the relative growth rate gradually reduced. At 100 mmol/L, cell migration rate wasn’t changed markedly, tube-like structure formation rate increased, thus promoting the vascularization of endothelial cells.

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