中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9181-.doi: 10.3969/j.issn.1673-8225.2010.49.014

• 干细胞移植 • 上一篇    下一篇

甲基强的松龙联合嗅鞘细胞移植对急性脊髓损伤大鼠运动功能和诱发电位的影响

王斌1，贺西京2，历 强3，王伟雄1，沈波1

 
  

  1. 1广州医学院第二附属医院创伤急诊外科，广东省广州市  510260；  2西安交通大学医学院第二附属医院骨二科，陕西省西安市 710004；3青岛市立医院东院骨科，山东省青岛市  266011
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 作者简介:王斌☆，男，1973年生，河南省安阳县人，汉族，2008年西安交通大学医学院毕业，博士，副主任医师，主要从事脊柱脊髓损伤和多发伤方面的研究。 wbhngz@126.com
  • 基金资助:

    广州医学院博士启动基金

Changes in motor function and evoked potentials of rats with acute spinal cord injury following olfactory ensheathing cells transplantation combined with Methyprednisolone

Wang Bin1, He Xi-jing2, Li Qiang3, Wang Wei-xiong1, Shen Bo1   

  1. 1 Department of Trauma Emergency Surgery, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou   510260, Guangdong Province, China; 2 Second Department of Orthopaedics, the Second Affiliated Hospital of Xi’an Jiaotong University Medical College, Xi’an   710004, Shaanxi Province, China; 3 Department of Orthopaedics, East Branch of Qingdao Municipal Hospital, Qingdao   266011, Shandong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Wang Bin☆, Doctor, Associate chief physician, Department of Trauma Emergency Surgery, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, Guangdong Province, China wbhngz@126.com
  • Supported by:

    Startup Foundation for Doctors in Guangzhou Medical College*

PDF

354

被引次数

11

摘要：

背景：嗅鞘细胞移植和甲基强的松龙是两种非常有前途的治疗脊髓损伤方法，关于二者联合治疗脊髓损伤的报道较少，结果也不尽相同。
目的：通过对大鼠行为学评分和诱发电位学检测了解嗅球嗅鞘细胞移植和甲基强的松龙对大鼠急性脊髓损伤的修复作用以及二者之间有无协同作用。
方法：以NYU脊髓打击法建立大鼠急性T10脊髓损伤模型，术后分别注射嗅鞘细胞、甲基强的松龙、嗅鞘细胞+甲基强的松龙、无血清的DF12培养液、生理盐水。于术后8周进行后肢体感诱发电位、运动诱发电位检测，并通过BBB评分了解各组大鼠手术前、后运动功能的变化。
结果与结论：术后8周，嗅鞘细胞组、甲基强的松龙组、嗅鞘细胞+甲基强的松龙组与损伤组、DF12组比较，大鼠后肢BBB评分明显升高，体感诱发电位、运动诱发电位 N1波潜伏期缩短，波幅升高，差异有显著性意义(P < 0.05)。嗅鞘细胞+甲基强的松龙组与嗅鞘细胞组、甲基强的松龙组比较，大鼠后肢BBB评分明显升高，体感诱发电位、运动诱发电位N1波潜伏期缩短，波幅升高，差异有显著性意义(P < 0.05)。说明嗅鞘细胞移植和甲基强的松龙单独应用均可以显著促进急性脊髓损伤大鼠运动功能恢复。二者联合促进急性脊髓损伤大鼠运动功能恢复的效果更加显著。

关键词: 甲基强的松龙, 脊髓损伤, 嗅鞘细胞, 协同作用, 行为学评分, 诱发电位学

Abstract:

BACKGROUND: Olfactory ensheathing cells (OECs) transplantation and Methyprednisolone (MP) are identified as two very promising methods in treating spinal cord injury (SCI). However, there are few reports about OECs combined with MP in treating SCI, and the treatment results are different.
OBJECTIVE: To investigate the behavioral score and electrophysiological effects of OECs transplantation and MP on the repair of acute spinal cord injury and whether there is a synergistic effect between OECs transplantation and MP.
METHODS: Acute spinal cord injury model (T10) was established by NYU impactor. All rats were randomly divided into 6 groups: control group, SCI group, DF12 group, OECs transplantation group, MP group, and OECs + MP group. Rats were injected with OECs, MP, OECs + MP, DF12 serum-free medium and saline, respectively after operation. At 8 weeks following operation, motion evoked potential (MEP) and somatosensory evoked potential (SEP) tests were performed. Changes in motor function before and after operation were estimated by Basso-Beattie-Bresnahan (BBB) score.
RESULTS AND CONCLUSION: Compared with the SCI and DF12 groups, BBB score was increased, the latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs, MP, and OECs + MP groups at 8 weeks following operation. Significant difference was detected (P < 0.05). The latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs + MP group compared with the OECs group and MP group, and significant difference was detected (P < 0.05). OECs transplantation and MP alone can significantly promote recovery of motor function of acute spinal cord injury rats. OECs transplantation combined with MP can more significantly promote recovery of the motor function of acute spinal cord injury rats.

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