中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8612-.doi: 10.3969/j.issn.1673-8225.2010.46.015

• 组织构建实验造模 • 上一篇    下一篇

人子宫内膜异位症裸鼠模型的血管生成

马颖，何援利   

  1. 南方医科大学珠江医院妇产科，广东省广州市 510282
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 何援利，女，1952年生，主任医师，教授，南方医科大学珠江医院妇产科，广东省广州市 510280 heyuanli310@yaoo.com.cn
  • 作者简介:马颖☆，女，1979年生，博士，2008年南方医科大学毕业，主治医师，主要从事妇科肿瘤及子宫内膜异位症的研究。 xxbcxh@sohu.com

Angiogenesis of a nude mouse model with human endometriosis

Ma Ying, He Yuan-li   

  1. Department of Gynecology and Obstetrics, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: He Yuan-li, Chief physician, Professor, Department of Gynecology and Obstetrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China heyuanli310@yaoo.com.cn
  • About author:Ma Ying☆, Doctor, Attending physician, Department of Gynecology and Obstetrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China xxbcxh@sohu.com

PDF

395

被引次数

8

摘要：

背景：有研究表明子宫内膜异位症的发生发展与新生血管关系密切，抗血管生成有可能成为治疗子宫内膜异位症的有效手段。
目的：建立人子宫内膜异位症裸鼠模型，观察异位病灶血管生成情况。
方法：采用腹腔注射人子宫内膜碎屑的方法建立子宫内膜异位症裸鼠模型，光镜下观察异位病灶的形态学特点，用免疫组织化学染色法检测裸鼠在位内膜及异位内膜血管内皮生长因子表达水平及微血管密度。
结果与结论：实验裸鼠异位病灶在光镜下呈现增生期子宫内膜形态特点；异位内膜较在位内膜血管内皮生长因子的表达有增多趋势，并有大量微血管形成。结果提示，实验成功建立了人子宫内膜异位症裸鼠模型，异位病灶中存在活跃的新生血管。

关键词: 子宫内膜异位症, 血管生成, 裸鼠模型, 微血管密度, 组织构建

Abstract:

BACKGROUND: Many studies indicated that angiogenesis play an important role in formation and development of endometriosis. Antiangiogenesis may be a useful way to treat endometriosis.
OBJECTIVE: To establish a nude mouse model with human endometriosis, and to observe the angiogenesis in ectopic focus.
METHODS: The nude mouse endometriosis model was established by abdominal cavity injection with the endometria of endometriosis patients. The morphological features of ectopic focus were observed by a light microscope, and the expression of vascular endothelial growth factor and microvessel density were detected by immunohistochemistry.
RESULTS AND CONCLUSION: The ectopic lesions were observed endometrium-liked structure with gland and substance under a light microscope. The expression of vascular endothelial growth factor in the ectopic endometrium was greater than that of eutopic endometrium, and many microvessels formed. It is suggested that, the endometriosis nude mice model was successfully established. There are new born vessels in ectopic lesions.

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