中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8563-.doi: 10.3969/j.issn.1673-8225.2010.46.004

• 组织构建细胞学实验 • 上一篇    下一篇

组织块培养法和酶消化法分离髌韧带细胞的比较

沈晓涛1, 2, 3, 4，陈斯韵1, 2, 3，赵宝寅1, 2, 3，郑  馨1, 2, 3，蔡冬青1, 2, 3, 4   

  1. 暨南大学，1再生医学教育部重点实验室，2再生医学香港中文大学暨南大学联合实验室，3科技部及广东省科技厅国际科技合作基地，4生物医学工程系 广东省广州市 510632
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 蔡冬青，教授，博士生导师，暨南大学，1再生医学教育部重点实验室，2再生医学香港中文大学暨南大学联合实验室，3科技部及广东省科技厅国际科技合作基地，4生物医学工程系，广东省广州市 510632 tdongbme@jnu.edu.cn
  • 作者简介:沈晓涛，女，1980年生，广东省湛江市人，汉族，助理实验师，主要从事韧带与肌腱分离培养以及损伤韧带与肌腱修复的研究。 tsxt@jnu.edu.cn
  • 基金资助:

    863项目(2007AA02Z105)，国家自然科学基金(30770886，30570369，30340038，30973158)；广东省自然科学基金重点项目(04105826)；广东省科技攻关项目(2004B30601007)。

Comparison of tenocytes isolated by tissue block culture and enzyme digestion

Shen Xiao-tao 1, 2, 3, 4, Chen Si-yun 1, 2, 3, Zhao Bao-yin 1, 2, 3, Zheng Xin 1, 2, 3 , Cai Dong-qing 1, 2, 3, 4   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Shen Xiao-tao, Assistant Technician, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China tsxt@jnu.edu.cn
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30770886*, 30570369*, 30340038*, 30973158*; the Natural Science Foundation of Guangdong Province, No. 04105826*; the Tackle Key Program in Science and Technology of Guangdong Province, No. 2004B30601007*

PDF

510

被引次数

11

摘要：

背景：调动和激活韧带与肌腱细胞群是有效修复损伤韧带与肌腱的关键，通过对韧带与肌腱细胞可能存在的不同群进行分离，对韧带与肌腱组织正常结构的维护及损伤修复具有重要意义。
目的：比较经组织块培养法和酶消化法分离出来的髌韧带细胞的形态、增殖，细胞复制性衰老与表面标记物表达。
方法：分别用组织块培养法和酶消化法对成年SD大鼠的髌韧带细胞进行分离培养，比较该两种方法得到的髌韧带细胞的形态，生长曲线，表面标记物的表达，细胞复制性衰老等情况。
结果与结论：经两种分离方法分离所得的髌韧带细胞的形态、增殖能力、表面标记物及复制性衰老均存在差异。提示髌韧带细胞可能存在不同群，且不同群的髌韧带细胞可能在髌韧带正常功能与修复中发挥不同的作用。

关键词: 韧带, 肌腱, 损伤, 修复, 韧带细胞

Abstract:

BACKGROUND: It is importance to activate tenocytes groups to repair the injured ligament and tendon. So, separating the different groups of tenocytes will elucidate the maintenance and repair of normal ligament and tendon structures.
OBJECTIVE: To compare the cell morphology, proliferation, replicative senescence and the cell surface markers expression of tenocytes isolated by tissue block culture and enzyme digestion.
METHODS: Tenocytes obtained from patellar tendon were isolated by tissue block culture and enzyme digestion. The cell morphology, proliferation, replicative senescence and the cell surface markers of isolated tenocytes were investigated.  
RESULTS AND CONCLUSION: There were significant differences between tenocytes isolated by tissue block culture and enzyme digestion in cell morphology, proliferation, replicative senescence and the cell surface markers. It suggested that tenocytes of patellar tendon might include different groups and the individual group of tenocytes may play the unique function in normal tendon function and tendon healing.

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