中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7649-7652.doi: 10.3969/j.issn.1673-8225.2010.41.012

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

大鼠骨骼肌糖酵解限速酶在不同训练负荷过程中的变化

张  敏1，陈立军1，靳秋月1，周  蔚2   

  1. 武警医学院，1生物化学教研室，  2研究生管理大队，天津市300162
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 陈立军，硕士，教授，武警医学院生物化学教研室，天津市 300162 chenlijun67@eyou.com
  • 作者简介:张敏★，女，1978年生，山东省济南市人，汉族， 2007年天津医科大学毕业，硕士，讲师，主要从事生物化学教学与研究工作。 zhangmin53421@163.com
  • 基金资助:

    本课题受武警医学院重点科研项目《不同训练负荷条件下身体机能的代谢评定及机制探讨》(WKH2009Z02)资助。

Variation of rate-limiting enzyme in glycolysis of rat skeletal muscle under different training intensities

Zhang Min1, Chen Li-jun1, Jin Qiu-yue1, Zhou Wei2   

  1. 1 Department of Biochemistry, 2 Graduate Management Brigade, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Chen Li-jun, Master, Professor, Department of Biochemistry, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China chenlijun67@eyou.com
  • About author:Zhang Min★, Master, Lecturer, Department of Biochemistry, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China zhangmin53421@163.com
  • Supported by:

    the Key Program of Medical College of Chinese People’s Armed Police Forces, No. WKH2009Z02*

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摘要：

背景：糖酵解系统在运动时能量的消耗和利用中起重要作用，不同的训练科目的主要供能系统不同，会引起战士机体不同的适应性变化。
目的：探讨不同训练负荷条件对大鼠骨骼肌相关糖酵解限速酶磷酸果糖激酶、己糖激酶和丙酮酸激酶活性的影响。
方法：参照BEDFORD TG标准，建立无氧、有氧和有氧无氧交替运动大鼠跑台训练模型，并设置正常对照组。各组动物训练结束后即刻处死，应用酶偶联法检测SD大鼠骨骼肌磷酸果糖激酶、己糖激酶和丙酮酸激酶的活性。
结果与结论：经过不同时间的跑台训练，无氧组大鼠骨骼肌磷酸果糖激酶活性均明显升高(P < 0.05)，交替运动组在训练至6周时磷酸果糖激酶活性增加(P < 0.05)，而有氧组磷酸果糖激酶活性在训练2周和4周后均下降(P < 0.05)。各组己糖激酶活性在训练4周和6周后均升高(P < 0.05)，其中无氧组最高(P < 0.05)，交替运动组己糖激酶活性高于有氧运动组(P < 0.05)。而无氧运动2和4周组丙酮酸激酶活性较对照组有所下降(P < 0.05)。 结果提示，大鼠骨骼肌糖酵解限速酶的活性不仅受运动方式的影响，而且在相同运动方式下还与训练时间的长短有关。长时间的训练，尤其是包含高速无氧训练的运动项目更能提高骨骼肌糖酵解限速酶的活性。

关键词: 训练, 有氧, 无氧, 糖酵解, 限速酶, 骨骼肌

Abstract:

BACKGROUND: Glycolytic system plays an important role in the consumption and utilization of energy in movement. Various training subjects lead to different adaptive changes of bodies.
OBJECTIVE: To explore the effects of different training intensities on phosphofructokinse (PFK), hexokinase (HK) and pyruvate kinase (PK) activities of rat skeletal muscle.
METHODS: According to BEDFORD TG standards, anaerobic exercise, aerobic exercise, aerobic and anaerobic cross-training motion models were established using treadmill running with different intensities. At the same time, normal animals served as control group. All the animals were killed immediately after exercise training. The activity of PFK, HK and PK were determined by enzyme-coupled assay.
RESULTS AND CONCLUSION: After treadmill training, the PFK activity in the anaerobic exercise group was increased (P < 0.05). To the cross-training group, it increased after 6 weeks’ training (P < 0.05), however, the activity of PFK was decreased after 2 and 4 weeks’ aerobic exercise training (P < 0.05). HK activity was increased after 4 and 6 weeks’ training (P < 0.05), which was the highest in the anaerobic exercise group (P < 0.05). Compared with the cross-training group, HK activity in the aerobic exercise group was lower (P < 0.05). The activity of PK was decreased in 2-week and 4-week anaerobic exercise group than that of the control group (P < 0.05). The present findings demonstrated that, the activity of rate-limiting enzyme in glycolysis was affected not only by exercise modes, but also by training periods. Long-time training, especially including high-speed anaerobic training, can protect the activity of rate-limiting enzyme in glycolysis.

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