中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (38): 7108-7111.doi: 10.3969/j.issn.1673-8225.2010.38.019

• 药物控释材料 drug delivery materials • 上一篇    下一篇

外部磁场介导下万古霉素磁性微球靶向治疗大鼠骨感染

王  文，蔡锦方，曹学成，孙宝国   

  1. 解放军济南军区总医院骨创伤外科，山东省济南市250031
  • 出版日期:2010-09-17 发布日期:2010-09-17
  • 作者简介:王文☆，男，1977年生，山东省汶上县人，汉族，2008年解放军第二军医大学毕业，博士，主治医师，主要从事骨创伤及显微外科研究。 wangwen007@163.com

Vancomycin microspheres of magnetic field compliance for targeted treatment of bone infection in rats

Wang Wen, Cai Jin-fang, Cao Xue-cheng, Sun Bao-guo   

  1. Department of Orthopaedic and Traumatic Surgery, General Hospital of Jinan Military Area Command of Chinese PLA, Jinan   250031, Shandong Province, China
  • Online:2010-09-17 Published:2010-09-17
  • About author:Wang Wen☆, Doctor, Attending physician, Department of Orthopaedic and Traumatic Surgery, General Hospital of Jinan Military Area Command of Chinese PLA, Jinan 250031, Shandong Province, China wangwen007@163.com

PDF

359

被引次数

10

摘要：

背景：对于骨感染的治疗，早期应用大剂量的敏感抗生素是一切治疗的基础。但现有局部应用抗生素的方法都存在着各种各样的缺点。
目的：制备具有磁场顺应性的万古霉素微球，并对其理化特性进行评价，观察具有磁场顺应性的万古霉素微球对大鼠骨感染的治疗效果。
方法：以壳聚糖作为万古霉素的载体，用乳化交联法制备具有磁场顺应性的万古霉素微球，通过扫描电镜及透射电镜观察微球形态，振动样品磁强计确证纳米微粒磁响应性的存在。观察外加磁场下具有磁场顺应性的万古霉素微球尾静脉注射后对大鼠急性骨感染的治疗效果以及大鼠胫骨X射线、病理改变，并与全身应用万古霉素的万古霉素治疗组对比分析。
结果与结论：成功制备了具有磁场顺应性的万古霉素微球，其外观呈规则球形，平均粒径298 nm，平均载药量为32.36%，平均包封率为80.9%，体外具有较好的磁场顺应性。外部磁场介导下，具有磁场顺应性的万古霉素微球尾静脉注射后可聚集在病灶区，明显抑制骨感染，其万古霉素的实际使用剂量仅为全身应用万古霉素剂量的1/3。万古霉素微球组与万古霉素治疗组的治疗效果差异无显著性意义。提示具有磁场顺应性的万古霉素微球是一种安全可靠的新型药物靶向制剂，为局部应用抗生素治疗骨感染开拓了新思路。

关键词: 磁性纳米微球, 壳聚糖, 万古霉素, 乳化交联法, 磁靶向给药系统, 骨感染, 靶向治疗

Abstract:

BACKGROUND: Massive use of sensitive antibiotics is the basis of all treatment for bone infection. However, current therapies of local applied antibiotics are not satisfactory.
OBJECTIVE: To prepare vancomycin microspheres with a magnetic field compliance, to evaluate the physico-chemical characteristics of the prepared nanoparticles, and to observe antibacterial effect of vancomycin microspheres with magnetic field compliance in the rat model of bone infection.
METHODS: Chitosan served as the carrier of vancomycin. The vancomycin microspheres with magnetic field compliance were prepared by emulsion-chemical crosslink method. The shape of nanoparticles was observed by scanning electron microscope and transmission electron microscope, the magnetism was determined by vibrating sample magnetometer. The therapeutic efficacy to bone infection by the caudal vein injection of vancomycin microspheres with magnetic field compliance was investigated, as well as the histological and pathological changes in the tibia of rat model. The control group was treated with general use of vancomycin.
RESULTS AND CONCLUSION: The vancomycin microspheres with magnetic field compliance have been prepared successfully. The microspheres had regular spherical surfaces, at the mean diameter of 100–350 nm, the mean drug loading of 32.36%, and the mean encapsulation ratio of 80.9%, and with a good magnetic response in vitro. The vancomycin microspheres with magnetic field compliance could obviously elevate drug concentration in targeting zone and inhibit bone infection, the actual vancomycin dosage of the vancomycin microspheres group was 1/3 of general usage group. There was no significant difference in the therapeutic effect between vancomycin microspheres group and general usage group. The vancomycin microspheres with magnetic field compliance are a safe and reliable drug delivery targeting system, and expand the local application of antibiotics for bone infection treatment.

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