中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6927-6930.doi: 10.3969/j.issn.1673-8225.2010.37.020

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

补骨脂素干预大鼠成骨细胞骨保护素/核因子κB受体激活因子配体mRNA的表达

王建华1，郭  敏1，郑  丽1，王  忠2   

  1. 1河北医科大学药学院，河北省石家庄市  050017；  2石药集团中诺药业有限公司，河北省石家庄市  050051
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 作者简介:王建华☆，男，1959年生，河北省石家庄人，汉族，2003年在河北医科大学毕业，博士，教授，主要从事中药对骨代谢的影响方面的研究。 smith_Wang2000@126.com
  • 基金资助:

    河北省自然科学基金资助项目(C2009001072)：补骨脂素防治去卵巢大鼠骨质疏松的实验研究及作用机制探讨；河北省科学技术研究与发展计划项目(河北省科技支撑计划项目 09276418D-14)：补骨脂素对大鼠骨代谢影响的实验研究；河北省中医药管理局课题 (2007116)：补骨脂素防治去卵巢大鼠骨质疏松的实验研究。

Effects of psoralen on osteoporogeterin and receptor activator nuclear factor kappa B ligand mRNA expression in rat osteoblasts

Wang Jian-hua1, Guo Min1, Zheng Li1, Wang Zhong2   

  1. 1 Pharmaceutical School of Hebei Medical University, Shijiazhuang  050017, Hebei Province, China; 2 Zhongnuo Pharmaceutical Co., Ltd., Shijiazhuang Pharmacy Group, Shijiazhuang  050051, Hebei Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Wang Jian-hua☆, Doctor, Professor, Pharmaceutical School of Hebei Medical University, Shijiazhuang 050017, Hebei Province, China smith_Wang2000@126.com
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. C2009001072*; the Plan for Research and Development of Science and Technology in Hebei Province, No. 09276418D-14*; Administration of Traditional Chinese Medicine of Hebei Province, No. 2007116*

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摘要：

背景：补骨脂素有促进大鼠成骨细胞增殖与分化的作用，但其作用机制尚不明确。
目的：探讨补骨脂素对大鼠成骨细胞中骨保护素(osteoporogeterin, OPG)和核因子κB受体激活因子配体(receptor activator nuclear factor kappa b ligand, RANKL) mRNA表达的影响。
方法：取第3代生长状况良好的出生24 h内的SD大鼠成骨细胞，补骨脂素组加入1×10-7 mol/L的补骨脂素，雌二醇组加入1×10-7 mol/L的雌二醇，对照组正常培养。给药72 h后提取细胞总RNA，RT-PCR方法分析细胞OPG/RANKL mRNA的表达。
结果与结论：与对照组比较，补骨脂素组和雌二醇组OPG mRNA的表达均明显增加(P < 0.05)，而RANKL mRNA的表达明显下降(P < 0.05)，但补骨脂素组成骨细胞OPG mRNA的表达较雌二醇组弱(P < 0.05)，VOPG/VRANKL比值也较雌二醇组小(P < 0.05)。说明补骨脂素可能通过增加成骨细胞OPG的表达，抑制RANKL的表达来抑制破骨细胞的分化和成熟，从而抑制骨吸收，达到防治骨质疏松症的目的，但作用不如雌二醇明显。

关键词: 补骨脂素, 大鼠成骨细胞, 骨保护素, 核因子&kappa, B受体激活因子配体, 植物雌激素, 骨质疏松症

Abstract:

BACKGROUND: Psoralen has effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro, but the mechanisms remain poorly understood.
OBJECTIVE: To investigate the effect of psoralen on the expression of osteoprotein (OPG) and receptor activator nuclear factor κB ligand (RANKL) in osteoblasts.
METHODS: Calvariae were obtained from Sprague-Dawley rats (within 24 hours after birth), bone samples were processed by collagenase/trypsin digestion, and the 3rd passage of osteoblasts were cultured by 1×10-7 mol/L psoralen or 1×10-7 mol/L estradiol. Cells in the control group were cultured normally. At 72 hours after culture, total mRNA was prepared from osteoblasts, and mRNA expression of OPG and RANKL were detected by RT-PCR.
RESULTS AND CONCLUSION: Compared with the control group, the expression of OPG mRNA in the psoralen and estradiol groups were obviously increased (P < 0.05), but the expression of RANKL mRNA were notably decreased (P < 0.05). The expression of OPG mRNA in the psoralen group was weaken than that of the estradiol group (P < 0.05), and the OPG/RANKL ratio was decreased in the psoralen group. Psoralen can prevent osteoporosis via increasing OPG expression and inhabiting RANKL level to suppress the osteoclasts differentiation and maturity, thus inhabiting bone resorption, but the effect is smaller than estradiol.

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