中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6891-6894.doi: 10.3969/j.issn.1673-8225.2010.37.012

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

复方茶多酚软膏对放射性皮炎大鼠伤口愈合和表皮生长因子表达的影响

薛  俐1，冯志明2，易银沙2，阎政礼2，李旭红1   

  1. 1中南大学湘雅三医院，湖南省长沙市  410013；2湖南师范大学制药工程系，湖南省长沙市  410081
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 冯志明，副教授，湖南师范大学制药工程系，湖南省长沙市 410081 fengzhiming@163.com.
  • 作者简介:薛俐，女，1958年生，湖南长沙人，汉族，1982年湖南中医学院毕业，副教授，主要从事中医药临床研究。 li0901@xy3yy.com
  • 基金资助:

    湖南省中医药科研计划资助项目(No. 2008096)，题目：复方茶多酚对放射性皮肤溃疡的的防治作用；湖南省科技计划资助项目(No. 2009SK3171)；题目：慢病毒介导CTGF-shRNA对放疗后皮肤成纤维细胞CTGF-mRNA的影响。

Effects of compound tea polyphenol ointment on wound healing and epidermal growth factor expression of radiation-induced cervical skin dermatitis in rats

Xue Li1, Feng Zhi-ming2, Yi Yin-sha2, Yan Zheng-li2, Li Xu-hong1　   

  1. 1 Third Hospital of Xiangya, Central South University, Changsha  410013, Hunan Province, China; 2 Department of Pharmaceutical Engineering, Hunan Normal University, Changsha  410081, Hunan Province, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Feng Zhi-ming, Associate professor, Department of Pharmaceutical Engineering, Hunan Normal University, Changsha 410081, Hunan Province, China fengzhiming@163.com
  • About author:Xue Li, Associate professor, Third Hospital of Xiangya, Central South University, Changsha 410013, Hunan Province, China li0901@xy3yy.com
  • Supported by:

    the Scientific Research Planning Project for Traditional Chinese Medicine in Hunan Province, No. 2008096*; Science and Technology Planning Project of Hunan Province, No. 2009SK3171*

PDF

493

被引次数

13

摘要：

背景：研究表明复方茶多酚软膏对急性放射性皮炎的伤口有愈合作用，但具体机制尚未阐明。
目的：探讨复方茶多酚软膏对大鼠放射性皮炎创面愈合及表皮生长因子表达的影响。
方法：清洁级雄性成年Wistar大鼠随机分成3组：实验组和对照组大鼠通过60Co γ射线照射建立大鼠放射性皮炎模型，实验组大鼠采用常规护理后用复方茶多酚软膏外涂辐射伤口区，对照组涂抹凡士林软膏；简单皮肤伤口组大鼠采用1.5 cm2皮肤全切除，仅接受常规护理。通过放射免疫法测定伤后5，10，20和40 d时创面组织的表皮生长因子表达、观测创面愈合率和愈合时间，并与简单伤口组比较。
结果与结论：实验组和对照组在γ射线照射后5 d创面组织表皮生长因子表达弱于简单皮肤伤口组，提示放射性皮炎与简单皮肤切口损失不同，创面恢复期创面组织表皮生长因子呈弱表达，愈合缓慢；实验组创伤后10和20 d时创面组织表皮生长因子表达显著高于对照组(P < 0.05)；实验组在10和20 d时的创面愈合率明显高于对照组(P < 0.05)；实验组愈合时间明显短于对照组的愈合时间(P < 0.05)，实验组和对照组的愈合时间均明显长于简单皮肤伤口组(P < 0.05)。结果表明复方茶多酚软膏外涂对大鼠放射性皮炎的愈合有促进作用，其机制可能与促进创面组织表皮生长因子的表达有关。

关键词: 放射性皮炎, 大鼠, 茶多酚, 黄芩苷, 表皮生长因子

Abstract:

BACKGROUND: Studies demonstrated that compound tea polyphenol ointment promotes wound healing in radiation-induced cervical skin dermatitis, but the precise mechanisms remain poorly understood. 
OBJECTIVE: To study the effects of compound tea polyphenol ointment on wound healing rate and epidermal growth factor (EGF) expression of radiation-induced dermatitis in rats.
METHODS: Adult Wistar rats were randomly divided into 3 groups. Rats in the experimental and control groups were established 60Coγ radiation-induced dermatitis models and treated by compound tea polyphenol ointment or Vaseline, respectively. In the simple wound group, 1.5-cm2 skin of rats were removed and treated by routine nursing care. The EGF expression in wound tissue was analyzed by radioimmunoassay, and the wound healing rate and wound healing time was also measured at 5, 10, 20, 30 and 40 days after radiation.
RESULTS AND CONCLUSION: The EGF expressions of wound skin tissues in the experiment and control groups were higher than that of the simple wound group at 5 days after radiation, which revealed that the radiodermatitis was different from simple wound and healed slowly. Compared with the control group, the EGF expression of wound skin tissues in the experiment group was significantly higher (P < 0.05) and the wound healing rate was significantly greater at 10 and 20 days after radiation(P < 0.05). The wound healing time of the experiment groups was significantly shorter than that of the control group (P < 0.05). But the wound healing time of the experiment and control groups was longer that that of the simple wound group (P < 0.05). Compound tea polyphenol ointment can significantly promote the wound healing of radiation-induced dermatitis in rats, which may be related to accelerating the EGF expression of wound skin tissues.

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