中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6882-6886.doi: 10.3969/j.issn.1673-8225.2010.37.010

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

表皮生长因子联合血糖控制对糖尿病创面愈合的影响：成纤维细胞生长因子蛋白及其mRNA表达的变化

宗守凯，梁自乾，纪雪亮   

  1. 广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市  530021
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 梁自乾，教授，硕士生导师，广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021 Liangzqian@yahoo.com
  • 作者简介:宗守凯★，男，1980年生，山东省日照市人，汉族，广西医科大学在读硕士，主要从事烧伤免疫及创面修复重建方面的研究。
  • 基金资助:

    广西壮族自治区科技厅资助项目(桂科自0542093)；广西研究生教育创新计划基金(2009105981002M198)。

Effect of epidermal growth factor combined with blood glucose control on wound healing in diabetes mellitus rats: Changes of fibroblast growth factor protein and its mRNA expressions

Zong Shou-kai, Liang Zi-qian, Ji Xue-liang   

  1. Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Liang Zi-qian, Professor, Master’s supervisor, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liangzqian@yahoo.com
  • About author:Zong Shou-kai★, Studying for master’s degree, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

     the Science and Technology Foundation of Guangxi Zhuang Autonomous Region, No. 0542093*; Graduate Education Innovation Fund of Guangxi Zhuang Autonomous Region, No. 2009105981002 M198*

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摘要：

背景：有研究表明急性创伤可导致内源性的重组人表皮生长因子(recombinant human epidermal growth factor，rhEGF)表达下调，导致不愈合的发生。
目的：观察局部应用rhEGF联合血糖控制下对糖尿病大鼠烫伤创面成纤维细胞生长因子表达的影响。
方法：将Wistar糖尿病大鼠制备背部深Ⅱ度烫伤模型。联合治疗组于烫伤前1周控制血糖至对照组水平，并在烫伤24 h内创面喷洒rhEGF；rhEGF组仅创面喷洒rhEGF；血糖控制组仅控制血糖；对照组不制备糖尿病模型，烫伤后处理同联合治疗组。烫伤后1，3，5，7，11，15，21 d取创面皮肤组织采用免疫组织化学染色及原位杂交法检测成纤维细胞生长因子蛋白及mRNA的表达，并观察各组大鼠创面愈合情况。
结果与结论：烫伤后各组大鼠创面成纤维细胞生长因子蛋白及mRNA表达均明显增多，分别在5~7 d及7~11 d达高峰，且联合治疗、对照组峰值较rhEGF、血糖控制组高(P < 0.05)。烫伤后7~21 d，联合治疗、对照组创面愈合率高于rhEGF、血糖控制组(P < 0.05)。说明局部应用rhEGF在联合血糖控制下可促进糖尿病大鼠烫伤创面的愈合，可能与促进创面成纤维细胞生长因子表达有关。

关键词: 糖尿病, 烫伤, 创面愈合, 重组人表皮生长因子, 成纤维细胞生长因子, 大鼠

Abstract:

BACKGROUND: Previous studies demonstrated that acute wound can result in down regulation of endogenic recombinant human epidermal growth factor (rhEGF) and lead to nonunion.
OBJECTIVE: To explore the effects of topical application of rhEGF combined with blood glucose control on the expression of fibroblast growth factor (FGF) on rat scald wound. 
METHODS: Wister diabetic rats were prepared for back deep Ⅱ degree scald models. The blood glucose levels of rats in the combination group were controlled to the levels of control group at 1 week before operation and were sprayed of rhEGF within 24 hours of scald. Rats in the rhEGF group only sprayed of rhEGF and the fasting blood glucose (FBG) were controlled in the FBG control group. The treatment in the control group was identical to the combination group. The healing rate of the wound was observed and expression of fibroblast growth factor (FGF) protein and mRNA were determined by immunohistochemistry and in situ hybridization at 1, 3, 5, 7, 11, 15 and 21 days after operation. 
RESULTS AND CONCLUSION: The expressions of FGF protein and mRNA in all rats were obviously increased in each group after scald, reached a peak at 5-7 and 7-11 days, respectively, in addition, the peak value of the combination and control groups were greater than that of the rhEGF and FBG control group (P < 0.05). The healing rates of the combination and control group were greater than that of the rhEGF and FBG control group at 7-21 days after scald (P < 0.05). The results demonstrated that external application of rhEGF combined with blood sugar control can accelerate obviously wound healing in diabetes mellitus rats, which may be related to the high expression of FGF.

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