中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4247-4251.doi: 10.3969/j.issn.1673-8225.2010.23.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

经5-氮胞苷诱导骨髓间充质干细胞心肌移植对心功能的影响

陈丽星1，聂 俊1，孙金华2，蔡红雁1，赵 玲1，郭 涛1，肖践明1   

  1. 1昆明医学院第一附属医院心内科，云南省昆明市  650031；
    2云南省妇幼保健院内科，云南省昆明市  650051
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 肖践明，教授，昆明医学院第一附属医院心内科，云南省昆明市 650031 Jianmingxiao@ 163.com
  • 作者简介:陈丽星，女，1977年生，汉族，云南省宣威市人，2009年中山大学毕业，博士，主治医师，主要从事冠心病基础研究。 cheng_l_x@sina.com
  • 基金资助:

    云南省教育厅基金资助项目(5Z0470C)

Effects of 5-azacytidine-induced bone marrow mesenchymal stem cells transplantation on cardiac function

Chen Li-xing1, Nie Jun1, Sun Jin-hua2, Cai Hong-yan1, Zhao Ling1, Guo Tao1, Xiao Jian-ming1   

  1. 1Department of Cardiology, First Affiliated Hospital, Kunming Medical College, Kunming  650031, Yunnan Province, China;
    2Department of Internal Medicine, Yunnan Provincial Maternal and Child Health Hospital, Kunming  650051, Yunnan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Xiao Jian-ming, Professor, Department of Cardiology, First Affiliated Hospital, Kunming Medical College, Kunming 650031, Yunnan Province, China Jianmingxiao@163. com
  • About author:Chen Li-xing, Doctor, Attending physician, Department of Cardiology, First Affiliated Hospital, Kunming Medical College, Kunming 650031, Yunnan Province, China cheng_l_x@sina.com
  • Supported by:

    a Grant from Department of Education of Yunnan Province, No. 5Z0470C*

PDF

377

被引次数

13

摘要：

背景：骨髓间充质干细胞具有多分化潜能，但其在体外经5-氮胞苷诱导后移植于急性心肌梗死大鼠中，能否改善近期及远期的心功能，目前尚不明确。
目的：观察兔骨髓间充质干细胞移植入急性心肌梗死组织后对心功能的影响。
方法：分离培养兔骨髓间充质干细胞，5-氮胞苷诱导4周后，体外DAPI标记。新西兰兔随机均分3组，假手术组：打开胸腔1 h后缝合胸腔；骨髓间充质干细胞组：于冠状动脉结扎建立急性心肌梗死模型后1 h在梗死周边区用微量注射器分4点注射诱导后的自体骨髓间充质干细胞；急性心肌梗死组：造模后于相同部位注射等量生理盐水。移植后3 d，4周超声心动图检测各组大鼠左室舒张末期容积，左室收缩末期容积及射血分数变化。
结果与结论：荧光显微镜观察发现，骨髓间充质干细胞组移植后3 d，4周心脏标本中均发现有DAPI标记的阳性细胞，呈散在分布于瘢痕周边区并沿心肌纤维排列方向走行。移植后3 d，骨髓间充质干细胞组与急性心肌梗死组相比，左室舒张末期容积、左室收缩末期容积及射血分数无明显差异，心功能没有明显的改善；移植4周后，骨髓间充质干细胞组与急性心肌梗死组相比，射血分数明显提高，左室舒张末期容积、左室收缩末期容积明显降低。

关键词: 骨髓间充质干细胞, 5-氮胞苷, 移植, 心肌梗死, 心功能

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have the potential to differentiate into various cells. BMSCs induced by 5-azacytidine were transplanted into myocardium of rabbits after myocardial infarction. Whether transplanted stem cells could improve cardiac function in short or long time was unclear.
OBJECTIVE: To investigate the effects of BMSCs transplantation on cardiac function in rabbits after acute myocardial infarction (AMI).
METHODS: Rabbit BMSCs were purified and cultured in vitro. BMSCs were induced by 5-azacytidine, and labeled with DAPI at 4 weeks following induction. New Zealand rabbits were randomly divided into three groups. In sham operation group, the chest was opened for 1 hour and then closed. In BMSCs group, autologous BMSCs were infused into the surrounding infarct area at 4 points using a microsyringe at 1 hour following model induction by coronary artery ligation. In AMI group, an equal volume of saline was injected at the same region following model establishment. At 3 days and 4 weeks following transplantation, ultrasonic cardiography was used to determine changes in left ventricular end diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF) in rats from each group.
RESULTS AND CONCLUSION: Fluorescence microscopy results demonstrated that DAPI-labeled BMSCs were distributed extensively in the myocardium of BMSCs group at 3 days and 4 weeks, arranged in parallel with the cardiac muscle fibers. At 3 days after transplantation, there were no significant differences in LVEDV, LVESV and LVEF between AMI and BMSCs groups. No significant improvement was found in cardiac function. At 4 weeks after transplantation, LVEDV and LVESV were significantly decreased, and LVEF was significantly increased in BMSCs group compared with AMI group.

中图分类号: