中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4243-4246.doi: 10.3969/j.issn.1673-8225.2010.23.015

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

DAPI标记骨髓间充质干细胞在胶质瘤模型大鼠脑内的迁徙和定位

于 音1，杨洪发2，邵佳甲3，邬 巍2，朱 东2，姜 涛1，郭永川1，梁前垒1，李衍鑫1   

  1. 1吉林大学白求恩第三临床学院，吉林省长春市 130031；
    2吉林大学白求恩第一临床学院，吉林省长春市 130021；  
    3长春职业技术学院，吉林省长春市 130031
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 杨洪发，副教授, 吉林大学白求恩第一临床学院，吉林省长春市 130021
  • 作者简介:于 音，男，1977年生，吉林省长春市人，汉族,2008年吉林大学毕业，博士,主要从事颅底肿瘤的显微外科治疗及相关疾病的干细胞、基因治疗研究。 fooish@sina.com

Migration and localization of bone marrow mesenchymal stem cells labeled by DAPI in the brain of glioma rat models

Yu Yin1, Yang Hong-fa2, Shao Jia-jia3, Wu Wei2, Zhu Dong2, Jiang Tao1, Guo Yong-chuan1, Liang Qian-lei1, Li Yan-xin1   

  1. 1Norman Bethune Third Clinical Hospital, Jilin University, Changchun  130031, Jilin Province, China;
    2Norman Bethune First Clinical Hospital, Jilin University, Changchun  130021, Jilin Province, China;
    3College of Occupation Technology, Changchun  130031, Jilin Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yang Hong-fa, Associate professor, Norman Bethune First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China
  • About author:Yu Yin, Doctor, Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China fooish@sina.com

PDF

587

被引次数

6

摘要：

背景：骨髓间充质干细胞作为载体细胞行脑内移植治疗胶质瘤，如何证实其为最好的药物载体？
目的：将DAPI标记的大鼠骨髓间充质干细胞移植入模型鼠脑内，观察骨髓间充质干细胞在肿瘤内的迁徙与定位。
方法: 体外培养骨髓间充质干细胞。利用立体定向仪将培养好的C6细胞注入大鼠脑内，建立大鼠脑内胶质瘤模型。将DAPI标记于培养好的骨髓间充质干细胞上，利用微量注射器注入模型鼠脑内；移植后第3，7天处死大鼠，利用共聚焦显微镜观察骨髓间充质干细胞在肿瘤内的分布及与肿瘤细胞的关系。
结果与结论:实验成功建立了大鼠脑内胶质瘤模型。以DAPI标记的骨髓间充质干细胞。在模型鼠脑内主动聚集于肿瘤血管周围，并能与肿瘤细胞发生融合。结果证实骨髓间充质干细胞可以作为肿瘤基因治疗的良好载体。

关键词: 胶质瘤, 骨髓间充质干细胞, 大鼠, DAPI标记, 脑

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) as vector cells were transplanted in the brain for treating glioma. How to verify BMSCs are best drug carriers.

OBJECTIVE: To observe the migration and localization of BMSCs in tumor following transplantation of rat BMSCs labeled by DAPI in the brain of a rat model.

METHODS: BMSCs were cultured in vitro. Using stereotaxic apparatus, C6 cells were injected into rat brain to establish glioma models. BMSCs labeled by DAPI were injected into the rat brain using a microinjector. The rats were sacrificed at 3 and 7 days following transplantation. The localization of BMSCs in tumor and the relationship with tumor cells were observed under a confocal microscope.

RESULTS AND CONCLUSION: The glioma model in rat brain was established successfully. BMSCs labeled by DAPI can accumulate around tumor vessel actively in rat brain, and can mix together with tumor cell. These suggested that BMSCs are a good carrier of gene therapy.

中图分类号: