中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (18): 3235-3238.doi: 10.3969/j.issn.1673-8225.2010.18.002

• 肝移植 liver transplantation • 上一篇    下一篇

自制PV液与大鼠肝脏低温保存：与UW液的对比

罗  刚，罗地来，李晓征，张荣传，朱栋良   

  1. 南昌大学第二附属医院肝胆外科，江西省南昌市   330006
  • 出版日期:2010-04-30 发布日期:2010-04-30
  • 通讯作者: 罗地来，教授，主任医师，硕士生导师，南昌大学第二附属医院肝胆外科，江西省南昌市 330006 45677185@qq.com
  • 作者简介:罗 刚★，男，1984年生，江西省万年县人，汉族，南昌大学医学院在读硕士，主要从事胆道、胰腺外科的研究。 ldilai868@163. com

Cryopreservation of rat liver in self-prepared PV solution: Compared with UW solution

Luo Gang, Luo Di-lai, Li Xiao-zheng, Zhang Rong-chuan, Zhu Dong-liang   

  1. Department of Hepatoliary Surgery, Second Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Luo Di-lai, Professor, Chief physician, Master’s supervisor, Department of Hepatoliary Surgery, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China 45677185@qq.com
  • About author:Luo Gang★, Studying for master’s degree, Department of Hepatoliary Surgery, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China ldilai868@163.com
  • Supported by:

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摘要：

背景：目前许多学者对UW液成分进行改进，其目的主要在于进一步探索器官保存的原理，提高保存的技术和延长保存的时限；简化UW液的成分，以进一步满足临床运输、储存和使用的方便；寻找合适的替代品，降低UW液的成本以满足市场需要。
目的：观察自制PV液对大鼠肝脏低温保存的效果，并与UW液进行对比。
方法：将90只Wistar大鼠按随机数字表法分成3组，即UW液组、PV液组和生理盐水组，制作大鼠肝脏非循环离体灌注模型，分别使用相应灌洗保存液。每组再根据不同的保存时间分为保存0，6，12，18，24 h 5个亚组，每个亚组6只大鼠。测定保存不同时间后肝脏谷丙转氨酶、谷草转氨酶、乳酸脱氢酶活性变化，以及灌注流出液中氧自由基代谢产物丙二醛浓度与超氧化物歧化酶活性；观察胆汁分泌量及镜下肝脏形态学变化。
结果与结论：PV液组与UW液组比较，灌注流出液中谷丙转氨酶、谷草转氨酶、乳酸脱氢酶、超氧化物歧化酶活性相近，差异无显著性意义(P > 0.05)。保存6 h后，PV液组肿瘤坏死因子α质量浓度显著高于UW液组(P < 0.05)；保存12 h后，UW液组丙二醛浓度显著高于PV液组(P < 0.05)；保存18 h后，PV液组胆汁分泌量低于UW液组(P < 0.05)；两组光镜、电镜下组织形态改变相似。提示PV液与UW液对Wistar大鼠肝脏功能均具有保护作用，两者短时间保存效果相似，在抗氧化及清除氧自由基方面，PV液略优于UW液。

关键词: 肝脏, 低温保存, 保存液, PV液, UW液

Abstract:

BACKGROUND: At present, many scholars make improvements to the UW solution composition, aiming to further explore the principle of organ preservation, improve preservation techniques and to extend the preservation time limit; simplified composition of UW solution can further meet the clinical transport, storage and ease of use; suitable alternatives should be found to reduce the cost of UW solution to satisfy the needs of the market.
OBJECTIVE: To discuss the effectiveness of self-prepared PV solution for preserving rat’s liver at a lower temperature, and to compare the results with UW solution.
METHODS: A total of 90 Wistar rats were randomly divided into three groups: UW group, PV group and normal saline (NS) group. Rats in each group were prepared for non-circulated isolated perfused rat liver models, and preserved for 0, 6, 12, 18, and 24 hours respectively, with 6 animals in each subgroup. The changes of hepatic enzymology (alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase) in corresponding time period, amount of metabolic production of oxyradicals from perfusion effluent liquid, and the bile secretion and changes in hepatic morphology under the microscope were observed.
RESULTS AND CONCLUSION: Amounts of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase and superoxide dismutase in perfusion effluent liquid were close between the PV group and UW group, and the difference was not significant (P > 0.05). After 6 hours of preservation, tumor necrosis factor α titre in the PV fluid group was greater than that in the UW group (P < 0.05); after 12 hours of preservation, amount of malonaldehyde in the UW group was evidently increased than that in the PV group (P < 0.05); after 18 hours of preservation, bile secretion in the PV group was lower than that in the UW group (P < 0.05); morphological changes of hepatic cells were similar in the two groups under the optical and electron microscope. The results demonstrated that PV solution and UW solution have a similar effect on protecting Wistar rat’s liver function, and PV solution is superior to UW solution in antioxidation and scavenging oxygen free radicals.

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