中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (6): 1101-1104.doi: 10.3969/j.issn.1673-8225.2010.06.032

• 干细胞学术探讨 • 上一篇    下一篇

复发性急性白血病患者血清和骨髓基质细胞培养上清液中血管细胞黏附分子1的变化

李学刚，侯丽君   

  1. 中山大学附属第五医院血液科，广东省珠海市 519000
  • 出版日期:2010-02-05 发布日期:2010-02-05
  • 通讯作者: 侯丽君，博士，教授，主任医师，中山大学附属第五医院血液科，广东省珠海市 519000
  • 作者简介:李学刚，男，1984年生，广东省珠海市人，汉族，2008年中山大学毕业，硕士，医师，主要从事血液病方面的研究。03451660@163.com
  • 基金资助:

    2007年广东省珠海市卫生局科研基金资助项目（2007041），课题名称“血管细胞黏附分子1在复发性急性白血病骨髓基质细胞中表达的研究”。

Changes of vascular cell adhesion molecule-1 in serum and marrow stroma cell supernatant of patients with relapsed acute leukemia

Li Xue-gang, Hou Li-jun   

  1. Department of Hematology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, Guangdong Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Hou Li-jun, Doctor, Professor, Chief physician, Department of Hematology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, Guangdong Province, China
  • About author:Li Xue-gang, Master, Physician, Department of Hematology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, Guangdong Province, China 03451660@163.com
  • Supported by:

    Scientific and Technological Research Foundation of Zhuhai Health Bureau, No. 2007041*

PDF

447

被引次数

4

摘要：

背景：研究认为急性白血病的复发可能与细胞黏附分子的异常表达密切相关。

目的：探讨复发性急性白血病患者血清和骨髓基质细胞培养上清液中血管细胞黏附分子1的水平变化。

方法：收集2006-06/2008-03在中山大学附属第五医院血液科住院期间同时具有缓解期和复发期的17例急性白血病患者的血清标本、骨髓基质细胞培养上清液标本。采用酶联免疫吸附双抗体夹心法测定处于缓解期和复发期的血清、骨髓基质细胞培养上清液标本中的血管细胞黏附分子1的水平。

结果与结论：与缓解期比较，急性白血病患者复发期血清中血管细胞黏附分子1的水平明显升高(P < 0.05)；体外培养1，3周的骨髓基质细胞培养上清液中血管细胞黏附分子1的水平无明显变化(P > 0.05)，第4周骨髓基质细胞培养上清液中血管细胞黏附分子1的水平明显升高(P < 0.05)。结果证实急性白血病患者复发期血清和骨髓基质细胞培养上清液中血管细胞黏附分子1表达水平均高于缓解期，推测血管细胞黏附分子1的异常表达可能与急性白血病的复发具有一定的相关性。

关键词: 急性白血病, 血管细胞黏附分子1, 骨髓基质细胞, 干细胞, 复发期, 缓解期

Abstract:

BACKGROUND: Relapse of acute leukemia is possibly correlated with abnormal expression of cellular adhesion molecule-1 (VCAM-1).

OBJECTIVE: To observe the level of VCAM-1 in serum and bone marrow stromal cell (MSC) supernatant of patients with relapsed acute leukemia.

METHODS: Samples of serum and MSC supernatant were collected from 17 patients with remission-phase and relapse-phase acute leukemia hospitalized in the Department of Hematology, the Fifth Affiliated Hospital of Sun Yat-sen University between June 2006 and March 2008. The levels of VCAM-1 were measured with ELISA in remission and relapse phases.

RESULTS AND CONCLUSION: Compared with the remission phase, the VCAM-1 level of serum was significantly increased in the relapse phase (P < 0.05). At 1 and 3 weeks after in vitro culture, the VCAM-1 level in the MSC supernatant was not changed (P > 0.05), but it was significantly increased on the fourth week (P < 0.05). The results demonstrated that VCAM-1 expression of serum and MSC supernatant in the relapse phase was greater than that in the remission phase, suggesting that abnormal expression of VCAM-1 was possibly correlated with relapse of acute leukemia.

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