中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (28): 4522-4527.doi: 10.12307/2024.464

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

脉冲电磁场可抑制老年大鼠膝关节软骨退变

尹林伟1，2，3，黄夏荣1，2，3，孙光华1，2，3，刘  静1，2，3，钟培瑞1，2，3，王金玲1，2，3，陈佳倩1，2，3，文  幸1，2，3，甘邵婷1，2，3，胡文韬1，2，3，李萌萌1，2，3，周  君1，2，3，4
  

  1. 1南华大学衡阳医学院附属第一医院，1康复医学科，2康复医学中心，3康复医学实验室，湖南省衡阳市  421001；4四川大学华西医院康复医学四川省重点实验室，四川省成都市  610041
  • 收稿日期:2023-07-12 接受日期:2023-09-06 出版日期:2024-10-08 发布日期:2023-11-27
  • 通讯作者: 周君，博士，博士生导师，主任医师，南华大学衡阳医学院附属第一医院，康复医学科，康复医学中心，康复医学实验室，湖南省衡阳市 421001；四川大学华西医院康复医学四川省重点实验室，四川省成都市 610041
  • 作者简介:尹林伟，男，1991年生，湖南省衡阳市人，汉族，主管治疗师，主要从事骨、关节疾病康复的基础与临床研究。
  • 基金资助:
    国家自然科学基金面上项目(81973917)，项目负责人：周君；湖南省临床医疗技术创新引导项目(2021SK51805)，项目负责人：周君；湖南省自然科学基金项目(2021JJ40493)，项目负责人：黄夏荣；湖南省卫生健康委员会重点项目(202103060198)，项目负责人：周君；南华大学临床医学4310项目，项目负责人：周君；南华大学校级重点项目子项目(USCKF201902K02)，项目负责人：周君

Pulsed electromagnetic fields inhibit knee cartilage degeneration in aged rats

Yin Linwei1, 2, 3, Huang Xiarong1, 2, 3, Sun Guanghua1, 2, 3, Liu Jing1, 2, 3, Zhong Peirui1, 2, 3, Wang Jinling1, 2, 3, Chen Jiaqian1, 2, 3, Wen Xing1, 2, 3, Gan Shaoting1, 2, 3, Hu Wentao1, 2, 3, Li Mengmeng1, 2, 3, Zhou Jun1, 2, 3, 4   

  1. 1Department of Rehabilitation, 2Rehabilitation Medicine Center, 3Rehabilitation Laboratory, the First Affiliated Hospital of Hengyang Medical School, University of South China, Hengyang 421001, Hunan Province, China; 4Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2023-07-12 Accepted:2023-09-06 Online:2024-10-08 Published:2023-11-27
  • Contact: Zhou Jun, MD, Doctoral supervisor, Chief physician, Department of Rehabilitation, Rehabilitation Medicine Center, Rehabilitation Laboratory, the First Affiliated Hospital of Hengyang Medical School, University of South China, Hengyang 421001, Hunan Province, China; Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Yin Linwei, Therapist-in-charge, Department of Rehabilitation, Rehabilitation Medicine Center, Rehabilitation Laboratory, the First Affiliated Hospital of Hengyang Medical School, University of South China, Hengyang 421001, Hunan Province, China
  • Supported by:
    National Natural Science Foundation of China, No. 81973917 (to ZJ); Hunan Provincial Clinical Medical Technology Innovation Guidance Project, No. 2021SK51805 (No. ZJ); Hunan Provincial Natural Science Foundation, No. 2021JJ40493 (to HXR); Hunan Provincial Health Commission Key Project, No. 202103060198 (to ZJ); Project 4310 of Clinical Medicine of University of South China (to ZJ); Key Sub-project of University of South China, No. USCKF201902K02 (to ZJ)

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摘要：


文题释义：

骨关节炎：是一种常见的与年龄相关的疾病，主要表现为关节疼痛、僵硬和功能障碍。目前治疗骨关节炎的方法包括药物治疗、物理治疗、手术等。
基质金属蛋白酶：是一类可降解基质组分的酶，主要参与细胞外基质的重塑和组织再建过程。基质金属蛋白酶可以降解胶原蛋白、纤维连接蛋白等基质组分，调节细胞外基质的合成和降解平衡，对于细胞迁移、组织修复、免疫应答等过程具有重要影响。在某些病理情况下，基质金属蛋白酶的活性过高可能导致细胞外基质破坏和病理损伤，通过调控基质金属蛋白酶的活性可以达到抑制病理过程的目的。


背景：脉冲电磁场作为一种重要的物理治疗方法对骨关节炎的疗效确切，但其作用机制尚未完全明确。

目的：观察脉冲电磁场对老年大鼠膝关节软骨退变的影响。
方法：选取8只6月龄SD大鼠作为青年组，正常饲养8周，不做任何处理；将16只22月龄SD大鼠随机分为老年组(n=8)和脉冲电磁场组(n=8)，对脉冲电磁场组大鼠进行脉冲电磁场干预，1次/d，每周5 d，连续干预 8周，老年组正常饲养8周，不做任何处理。8周后麻醉处死所有大鼠，进行相关指标的检测。

结果与结论：①与青年组相比，老年组大鼠血清Ⅱ型胶原C端肽水平升高(P < 0.05)；与老年组相比，脉冲电磁场组大鼠血清Ⅱ型胶原C端肽水平降低(P < 0.05)；②显微CT显示，与青年组相比，老年组大鼠胫骨的骨体积分数、骨密度、骨小梁数量均降低(P < 0.05)，骨小梁间隙增大(P < 0.05)；与老年组相比，脉冲电磁场组大鼠胫骨的骨体积分数、骨密度、骨小梁数量均升高(P < 0.05)，骨小梁间隙减少(P < 0.05)；③胫骨平台番红O-固绿染色显示，老年组大鼠关节软骨结构紊乱，软骨细胞明显减少，无法区分潮线；脉冲电磁场组上述情况有所改善；④RT-qPCR与Western blot检测显示，与青年组相比，老年组大鼠关节软骨及软骨下骨中基质金属蛋白酶1、基质金属蛋白酶13、P53、P21 的mRNA与蛋白表达均升高(P < 0.05)；与老年组相比，脉冲电磁场组大鼠关节软骨及软骨下骨中基质金属蛋白酶1、基质金属蛋白酶13、P53、P21 的mRNA与蛋白表达均降低(P < 0.05)；⑤结果表明，脉冲电磁场可能通过抑制P53/P21的表达抑制软骨细胞衰老，缓解关节软骨降解及抑制软骨下骨骨质疏松，改善老年大鼠骨关节炎。

https://orcid.org/0000-0001-7172-977X(尹林伟)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 脉冲电磁场, 老年大鼠, 膝骨关节炎, 关节软骨退变, P53, P21

Abstract: BACKGROUND: Pulsed electromagnetic fields, as an important physical therapy, are exactly effective in the treatment of osteoarthritis, but the mechanism has not been fully clarified.
OBJECTIVE: To observe the effect of pulsed electromagnetic field on the degeneration of knee joint cartilage in aged rats.
METHODS: Eight 6-month-old Sprague-Dawley rats were selected as the young group and were subjected to normal diet with no treatment. Sixteen 22-month-old Sprague-Dawley rats were randomly divided into old group (n=8) and pulsed electromagnetic field group (n=8). The rats in the pulsed electromagnetic field group were subjected to a pulsed electromagnetic field intervention, once a day, 5 days per week for continuous 8 weeks. The rats in the old group were given no treatment. All rats were anesthetized and executed after 8 weeks for the detection of relevant indexes.
RESULTS AND CONCLUSION: Compared with the young group, serum type II collagen C-terminal peptide level was increased in the old group (P < 0.05); compared with the old group, serum type II collagen C-terminal peptide level was decreased in the pulsed electromagnetic field group (P < 0.05). Micro-CT showed that the bone volume fraction, bone mineral density, and number of bone trabeculae decreased (P < 0.05) and the trabecular separation increased 
(P < 0.05) in the tibia of rats in the aged group compared with the young group; and the bone volume fraction, bone density, and number of trabeculae increased (P < 0.05) and the trabecular separation decreased (P < 0.05) in the tibia of rats in the pulsed electromagnetic field group compared with the aged group. The tibial plateau Safranin O-fast green staining showed that the articular cartilage structure of rats in the aged group was disorganized, and the number of chondrocytes was obviously reduced, and the tidal line could not be distinguished. The above results were improved in the pulsed electromagnetic field group. RT-qPCR and western blot assay showed that the mRNA and protein expression levels of matrix metalloproteinase 1, matrix metalloproteinase 13, P53 and P21 in the articular cartilage and subchondral bone of rats were elevated in the aged group compared with the young group (P < 0.05) and decreased in the pulsed electromagnetic field group compared with the old group (P < 0.05). To conclude, pulsed electromagnetic fields may improve osteoarthritis in aged rats by inhibiting chondrocyte senescence, alleviating articular cartilage degradation and inhibiting subchondral bone osteoporosis through suppressing the expression of P53/P21. 

Key words: pulsed electromagnetic field, aged rat, knee osteoarthritis, articular cartilage degeneration, P53, P21

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