中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (15): 2302-2306.doi: 10.12307/2024.402

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

碱热处理纯钛表面纳米改性后对成骨细胞早期黏附生长的影响

高  岩1，林  曦1，刘  影2   

  1. 南方医科大学口腔医院(口腔医学院)，1种植中心，2牙体牙髓科，广东省广州市  510280
  • 收稿日期:2023-05-13 接受日期:2023-07-08 出版日期:2024-05-28 发布日期:2023-09-19
  • 通讯作者: 刘影，博士，副主任医师，南方医科大学口腔医院(口腔医学院)牙体牙髓科，广东省广州市 510280
  • 作者简介:高岩，男，1984年生，广东省广州市人，汉族，博士，副主任医师，主要从事钛种植体表面处理及骨替代品研究。
  • 基金资助:
    广东省自然科学基金项目(2018A030310439)，项目负责人：高岩；广东省医学科学技术研究基金项目(A2021480)，项目负责人：高岩；广东省医学科学技术研究基金项目(B2021061)，项目负责人：林曦

Effect of nano-modified titanium surface with alkali heat treatment on early adhesion and growth of osteoblasts

Gao Yan1, Lin Xi1, Liu Ying2   

  1. 1Implant Center, 2Department of Dentistry and Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou 510280, Guangdong Province, China
  • Received:2023-05-13 Accepted:2023-07-08 Online:2024-05-28 Published:2023-09-19
  • Contact: Liu Ying, MD, Associate chief physician, Department of Dentistry and Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou 510280, Guangdong Province, China
  • About author:Gao Yan, MD, Associate chief physician, Implant Center, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou 510280, Guangdong Province, China
  • Supported by:
    Natural Science Foundation of Guangdong Province, No. 2018A030310439 (to GY); Guangdong Medical Science and Technology Research Fund Project, No. A2021480 (to GY); Guangdong Medical Science and Technology Research Fund Project, No. B2021061 (to LX)

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摘要：


文题释义：

碱热处理：将钛、钽等金属置于强碱中一段时间后取出，再经高温处理后形成具有一定生物学活性表面的化学改性技术。经碱热处理的植入体表现出很好的血液相容性，而且会在表面形成具有生物活性的钛酸钠层，具有较好的生物活性。
亲水性：是指带有极性基团的分子对水有较大的亲和能力，可以吸引水分子或易溶解水。材料表面亲水性是一种界面现象，实质是物质界面发生性质和能量的变化。当水分子之间的内聚力小于水分子与材料分子间的相互吸引力时，材料被水润湿。研究显示亲水性材料表面具有一定的生物活性。


背景：纯钛表面纳米结构改性是钛种植体表面处理研究的热点领域。

目的：评价碱热处理纯钛表面纳米改性后对成骨细胞早期黏附生长的影响。
方法：取直径15 mm、厚度1.5 mm的四级纯钛片，分3组处理：光滑处理组经250目、800目、1 500目碳化硅砂纸逐级打磨抛光；喷砂酸蚀处理组在0.45 MPa气压下用100 μm的Al2O3颗粒对光滑处理后的钛片进行喷砂处理，然后进行酸蚀处理；碱热处理组将经过光滑处理的钛片置于反应釜内，加入10 mol/L NaOH溶液浸没钛片，置于100 ℃烘箱中加热12 h。检测3组钛片的表面形貌、粗糙度及亲水性。将成骨细胞MG63分别接种于3组钛片表面，免疫荧光染色观察细胞黏附情况。

结果与结论：①扫描电镜显示，光滑处理组钛片表面带有均匀的划痕，喷砂酸蚀处理组钛片表面凹凸不平，碱热处理组钛片表面形成均匀一致的纳米级三维孔洞形貌；喷砂酸蚀处理组、碱热处理组钛片的粗糙度值大于光滑组(P < 0.05)，水接触角低于光滑处理组(P < 0.05)；②MG63细胞接种3，6 h后的免疫荧光染色显示，喷砂酸蚀处理组、碱热处理组钛片表面的黏附细胞数量高于光滑处理组(P < 0.05)；接种12 h后的免疫荧光染色显示，相对于光滑处理组，喷砂酸蚀处理组和碱热处理组钛片表面的细胞肌动蛋白骨架更加伸展，大部分细胞伸出较粗壮的伪足，利于后续细胞间信号传导和细胞间相互作用；③结果表明，碱热处理可在钛片表面制备出具有一定生物活性的纳米结构，有利于成骨细胞的早期黏附。

https://orcid.org/0009-0008-6175-4080(高岩)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 钛片, 纳米结构, 碱热处理, 喷砂酸蚀, 成骨细胞, 生物材料

Abstract: BACKGROUND: Nanostructure modification of pure titanium surface is a hot research field of titanium implant surface treatment. 
OBJECTIVE: To evaluate the effect of nano-modified titanium surface treated with alkali heat treatment on early adhesion and growth of osteoblasts.
METHODS: Four-grade pure titanium sheets with a diameter of 15 mm and a thickness of 1.5 mm were taken and processed in three groups: the smooth treatment group was polished step by step with 250 mesh, 800 mesh, and 1 500 mesh silicon carbide sandpaper. In the sandblasting group, the smoothed titanium sheet was sandblasted with 100 μm Al2O3 particles at 0.45 MPa pressure, and then the acid etching was carried out. In the alkali heat treatment group, the smoothed titanium sheet was placed in the reactor, immersed in 10 mol/L NaOH solution, and heated in the oven at 100 °C for 12 hours. The surface morphology, roughness, and hydrophilicity of three groups of titanium sheets were measured. MG63 osteoblasts were inoculated on the surface of three groups of titanium tablets, and the adhesion of the cells was observed by immunofluorescence staining. 
RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that the surface of titanium sheets in the smooth treatment group had uniform scratches; the surface of titanium sheets in the sandblasting group was uneven, and the surface of titanium sheets in the alkali heat treatment group had uniform nanoscale three-dimensional pore morphology. The roughness value of the titanium sheets in sandblasting and alkali heat treatment groups was higher than that in the smooth treatment group (P < 0.05), and the water contact angle was lower than that in the smooth treatment group (P < 0.05). (2) Immunofluorescence staining after 3 and 6 hours of inoculation of MG63 cells showed that the number of adhesion cells on the surface of the titanium sheet in the sandblasting group and alkali heat treatment group was higher than that in the smooth treatment group (P < 0.05). Immunofluorescence staining 12 hours after inoculation showed that compared with the smooth treatment group, the actin skeleton of cells on the surface of titanium sheets in the sandblasting group and alkali heat treatment group was more extended, and most cells extended stronger pseudopodia, which was conducive to subsequent intercellular signal transduction and intercellular interaction. (3) The results showed that the nanostructures with certain biological activity could be prepared on the surface of a titanium sheet by alkali heat treatment, which was conducive to the early adhesion of osteoblasts.

Key words: titanium surface, nanostructure, alkali heat treatment, sandblasting acid etching, osteoblast, biomaterial

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