中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (10): 1547-1552.doi: 10.12307/2023.305

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

聚乙烯亚胺/氧化石墨烯/苯丙氨酸手性界面对应激耐受多系分化细胞黏附和增殖的影响

周晶晶1，2，孙一新1，2，盛  扬1，2，张  嵘1，2   

  1. 1常州大学材料科学与工程学院，江苏省常州市   213614；2江苏省环境友好型高分子材料重点实验室，常州大学，江苏省常州市   213164 
  • 收稿日期:2022-04-02 接受日期:2022-06-06 出版日期:2023-04-08 发布日期:2022-09-08
  • 通讯作者: 孙一新，博士，副教授，常州大学材料科学与工程学院，江苏省常州市 213614；江苏省环境友好型高分子材料重点实验室，常州大学，江苏省常州市 213164
  • 作者简介:周晶晶，女，1996年生，河南省濮阳市人，汉族，常州大学在读硕士，主要从事手性生物材料的研究。

Effects of chiral polyethyleneimine/graphene oxide/phenylalanine interface on adhesion and proliferation of multilineage-differentiating stress enduring cells

Zhou Jingjing1, 2, Sun Yixin1, 2, Sheng Yang1, 2, Zhang Rong1, 2   

  1. 1School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; 2Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China
  • Received:2022-04-02 Accepted:2022-06-06 Online:2023-04-08 Published:2022-09-08
  • Contact: Sun Yixin, MD, Associate professor, School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China
  • About author:Zhou Jingjing, Master candidate, School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China

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摘要：

文题释义：
手性生物界面：一种具有手性结构的表面，是与生物活性分子发生相互作用的主要场所，表面的形貌、成分以及固定活性分子的数量均影响着材料与生物分子相互作用的方式和强弱。
Muse细胞：是一种遍布全身可以从骨髓、脂肪、真皮以及结缔组织中分离，也可以从成纤维细胞和间充质细胞中分离的新型干细胞，可自发分化为3个胚层，具有低致瘤性，在再生医学领域表现出巨大的应用前景。

背景：近年来，应激耐受多系分化(Muse)细胞因其多能分化能力和非致瘤性而受到越来越多的关注，Muse细胞治疗在诸多疾病的动物实验中显示出较好的结果。然而，在长期的体外培养中，Muse细胞面临着增殖速度较慢的问题。
目的：研究材料手性对Muse细胞黏附与增殖的影响。
方法：通过层层自组装方法，以聚乙烯亚胺(PEI)作为基底层，氧化石墨烯(GO)和 L/D-苯丙氨酸(L/D-Phe)顺序沉积形成聚乙烯亚胺/氧化石墨烯/苯丙氨酸手性生物界面(PEI/GO/L-Phe和PEI/GO/D-Phe)。研究自组装手性生物界面对Muse细胞黏附、增殖行为的影响。
结果与结论：①通过简单的层层自组装技术构建了PEI/GO/L-Phe 和 PEI/GO/D-Phe手性界面；②统计结果显示：材料界面手性极大地影响了Muse细胞的行为，PEI/GO/L-Phe界面有利于Muse细胞的黏附和增殖，这可能由于该界面能吸附更多的蛋白，进一步促进了Muse细胞的增殖。

https://orcid.org/0000-0003-3487-2408 (孙一新) 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 再生医学, Muse细胞, 手性界面, 层层自组装, 苯丙氨酸对映体, 黏附, 增殖, 蛋白质吸附

Abstract: BACKGROUND: In recent years, multilineage-differentiating stress enduring (Muse) cell has attracted increasing attention due to its pluripotent differentiation capacity and non-tumorigenicity. Cell therapy based on Muse cells has shown exciting results in the animal experiments of many diseases. However, Muse cells face the difficulties of slow proliferation in long-term in vitro culture.  
OBJECTIVE: To investigate the effect of chirals interfaces on adhesion and proliferation of Muse cells.
METHODS:  The polyethyleneimine/graphene oxide/phenylalanine chiral biointerfaces (PEI/GO/L-Phe and PEI/GO/D-Phe) were constructed based on the layer-by-layer self-assembly method using polyethyleneimine (PEI) as the substrate layer and graphene oxide (GO) and L/D-phenylalanine (L/D-Phe) sequentially deposited. The effect of self-assembled chiral biointerfaces on the adhesion and proliferation behavior of Muse cells was investigated.  
RESULTS AND CONCLUSION: (1) PEI/GO/L-Phe and PEI/GO/D-Phe chiral interfaces were successfully constructed based on facile self-assembly technology. (2) The statistical results showed that the chirality on the interface influenced Muse cell behaviors significantly. The initial adhesion and subsequent proliferation of Muse cells were enhanced by the PEI/GO/L-Phe interface, probably owing to the preferred adsorption of proteins, which further promote Muse cell proliferation.

Key words: regenerative medicine, Muse cells, chiral interfaces, layer self-assembly, phenylalanine enantiomers, adhesion, proliferation, protein adsorption

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