中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (23): 3744-3749.doi: 10.12307/2022.677

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

短期负荷下p38抑制剂对兔髌腱及止点炎症因子的影响

齐亚楠1，陈一言2，张  宁3，王  琳3   

  1. 1西安交通大学城市学院，陕西省西安市   710018；2苏州大学，江苏省苏州市   215325；3北京体育大学，北京市   100084
  • 收稿日期:2021-09-04 接受日期:2021-10-20 出版日期:2022-08-18 发布日期:2022-02-15
  • 通讯作者: 王琳，教授，博士生导师，北京体育大学，北京市 100084
  • 作者简介:齐亚楠，女，1993年生，2019年北京体育大学毕业，硕士，主要从事运动损伤的机理与康复研究。
  • 基金资助:
    北京体育大学校级课题(2017XS018)，项目负责人：齐亚楠

Effect of p38 inhibitor on inflammatory factors in the patellar tendon and patella-patellar tendon junction under short-term exercise

Qi Yanan1, Chen Yiyan2, Zhang Ning3, Wang Lin3   

  1. 1City College of Xi’an Jiaotong University, Xi’an 710018, Shaanxi Province, China; 2Soochow University, Suzhou 215325, Jiangsu Province, China; 3Beijing Sport University, Beijing 100084, China
  • Received:2021-09-04 Accepted:2021-10-20 Online:2022-08-18 Published:2022-02-15
  • Contact: Wang Lin, Professor, Doctoral supervisor, Beijing Sport University, Beijing 100084, China
  • About author:Qi Yanan, Master, City College of Xi’an Jiaotong University, Xi’an 710018, Shaanxi Province, China
  • Supported by:
    a grant from Beijing Sport University, No. 2017XS018 (to QYN)

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摘要：

文题释义：
p38丝裂原活化蛋白激酶：广泛存在于多种组织细胞之中，不仅参与了炎症反应，调控机体应激状况下炎症相关的基因表达，而且还与细胞骨架重构、细胞增殖与凋亡以及细胞的能量代谢有关。
p38抑制剂：目前人工合成的p38抑制剂在化学结构上分为两类，一类是吡啶咪唑芳基杂环类化合物，其中SB203580应用比较广泛，其他化合物均在其分子基础上进行改造，解决原有化合物的选择性及毒性问题。

背景：p38抑制剂干预治疗相关疾病已经取得了很好的成效，但是关于短期大强度负荷下p38抑制剂对兔髌腱及髌骨髌腱结合部中炎性反应的影响仍有许多疑问。
目的：在实验室建立的定量跳跃动物模型上，观察短期内大强度运动负荷下，p38抑制剂对兔髌腱及髌骨髌腱结合部中主要炎性因子白细胞介素1β、白细胞介素6和转化生长因子β1表达的影响。
方法：34只18周龄新西兰大白兔，随机分为对照组(n=4)、跳跃组1，3，5 d(n=5)及跳跃+p38抑制剂组1，3，5 d(n=5)。各跳跃组进行电刺激跳跃训练，每次电刺激引发兔向前上方跳跃，跳跃高度达到10 cm定义为合格，每次训练共进行150次合格跳跃。跳跃+p38抑制剂组在每次训练后腹腔注射SB203580(0.5 mg/kg)，对照组不训练，预适应过程及饲养时间相同。各组训练结束后麻醉处死取材，采用SABC法对组织进行免疫组织化学染色，并通过Metamorph图形处理软件对各炎症因子的阳性细胞密度进行定量计算。
结果与结论：①髌腱及髌骨髌腱结合部中白细胞介素1β、白细胞介素6和转化生长因子β1的免疫组化结果显示，短期大强度负荷运动下髌腱及髌骨髌腱结合部的炎症因子变化情况不一致；②与跳跃组相比，跳跃+p38抑制剂组髌腱与髌骨髌腱结合部中3个炎症因子白细胞介素1β、白细胞介素6和转化生长因子β1的表达水平均没有显著变化(P > 0.05)；③结果提示大强度运动后急性期p38抑制剂可能不会影响髌腱及腱止点的炎症反应。
缩略语：丝裂原活化蛋白激酶：mitogen activated protein kinase，MAPK；转化生长因子β1：transforming growth factor-β1，TGF-β1

https://orcid.org/0000-0002-1923-5139 (齐亚楠) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 短期负荷, 髌腱, 髌骨髌腱结合部, 炎症因子, p38抑制剂

Abstract: BACKGROUND: p38 inhibitors have achieved good results in the intervention and treatment of related diseases. However, there are still many questions about the effect of p38 inhibitors on the inflammatory response in the patellar tendon and the patella-patellar tendon junction under short-term high-intensity exercise.
OBJECTIVE: On the basis of a quantitative jumping animal model successfully established in the laboratory, to investigate the effect of p38 inhibitors on the expression of main inflammatory factors, including interleukin-1β, interleukin-6 and transforming growth factor-β1 in the patellar tendon and patella-patellar tendon junction under short-term high-intensity exercise.
METHODS: Thirty-four 18-week-old New Zealand white rabbits were randomly divided into control group (n=4); jump 1, 3, 5 days groups (n=5 per group); jump+p38 inhibition 1, 3, 5 days groups (n=5 per group). Each jump group was subjected to electrical stimulation-induced jump training (jumping forward and upward), with a jump height of 10 cm defined as a qualified jump. Each training sessions included 150 qualified jumps. SB203580 (0.5 mg/kg) was intraperitoneally injected in each jump+p38 inhibition group after each training. The control group did not perform jump training, and the experimental group received electrical stimulation jump training according to the training program. The pre-adaptation process and feeding time were the same. Except for the control group, the rabbits from the other groups were sacrificed after training, and immunohistochemical staining was performed using the SABC method, and the density of the positive cells of each inflammatory factor was quantitatively calculated by Metamorph graphics processing software.
RESULTS AND CONCLUSION: Immunohistochemistry results indicated inconsistent changes in the expression of interleukin-1β, interleukin-6 and transforming growth factor-β1 in the patellar tendon and patella-patellar tendon junction under short-term high-intensity exercise. Compared with the jump groups, the expressions of interleukin-1β, interleukin-6 and transforming growth factor-β1 in the patellar tendon and patella-patellar tendon junction were not significantly different in each jump+p38 inhibitor group (P > 0.05). These findings reveal that the p38 inhibitor may not affect the inflammatory response of the patella tendon and patella-patellar tendon junction in the acute phase under short-term high-intensity exercise.

Key words: short-term exercise, patellar tendon, patella-patellar tendon junction, inflammatory factor, p38 inhibitor

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