中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (21): 3319-3326.doi: 10.12307/2022.638

• 组织工程骨材料Tissue-engineered bone • 上一篇    下一篇

氨基改性人造颌骨纳米羟基磷灰石/聚乳酸复合材料的制备与表征

刘龙珠1，龙远铸2，杨成雪1，仲欣琪1，汪一芳1，刘建国1   

  1. 1遵义医科大学口腔医学院，贵州省遵义市  563099；2遵义医科大学生命科学研究院，贵州省遵义市  563006
  • 收稿日期:2021-09-09 接受日期:2021-10-28 出版日期:2022-07-28 发布日期:2022-01-27
  • 通讯作者: 刘建国，教授，博士生导师，遵义医科大学口腔医学院，贵州省遵义市 563099
  • 作者简介:刘龙珠，女，1994年生，湖南省株洲市人，汉族，遵义医科大学在读硕士，医师，主要从事口腔材料及牙列缺损缺失修复方面的研究。
  • 基金资助:
    国家重点研发计划项目(2016YFC1102800)，项目参与者：刘建国；贵州省医用生物材料研发人才基地(黔人领发[2018]3号)，项目负责人：刘建国；遵义市医用生物材料研发创新人才基地(遵委[2019]69号)，项目负责人：刘建国

Preparation and characterization of the composites of amino-modified artificial jaw nano-hydroxyapatite/polylactic acid

Liu Longzhu1, Long Yuanzhu2, Yang Chengxue1, Zhong Xinqi1, Wang Yifang1, Liu Jianguo1   

  1. 1School of Stomatology, Zunyi Medical University, Zunyi 563099, Guizhou Province, China; 2Institute of Life Sciences, Zunyi Medical University, Zunyi 563006, Guizhou Province, China
  • Received:2021-09-09 Accepted:2021-10-28 Online:2022-07-28 Published:2022-01-27
  • Contact: Liu Jianguo, Professor, Doctoral supervisor, School of Stomatology, Zunyi Medical University, Zunyi 563099, Guizhou Province, China
  • About author:Liu Longzhu, Master candidate, Physician, School of Stomatology, Zunyi Medical University, Zunyi 563099, Guizhou Province, China
  • Supported by:
    National Key Research and Development Project, No. 2016YFC1102800 (to LJG); Guizhou Medical Biomaterials Research and Development Talent Base, No. [2018]3 (to LJG); Zunyi Medical Biomaterials Research and Development Innovative Talent Base, No. [2019]69 (to LJG)

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摘要：

文题释义：
氨基改性：通过引入带有氨基的物质改变原有材料形态或性质的方法。
人工骨：是指可以替代人体骨或者修复骨组织缺损的人工生物材料。

背景：口腔颌骨缺损修复常选择骨移植材料和人工骨替代材料两种，骨移植材料有其优点，但存在免疫反应、来源不足等缺陷，人工骨替代材料作为骨缺损修复材料的应用潜力巨大。
目的：探讨侧链接枝氨基改性纳米羟基磷灰石/聚乳酸复合材料作为口腔人造颌骨材料的制备方法，并进行表征。 
方法：在纳米羟基磷灰石表面赋予氨基，再进行接枝改性，将接枝改性的纳米羟基磷灰石与聚乳酸复合，制作人造颌骨材料，复合材料中接枝改性纳米羟基磷灰石的含量分别为10%，30%，50%，表征材料的理化性能、机械力学性能与细胞相容性。
结果与结论：①核磁氢谱、傅里叶变换红外光谱、X射线衍射及热失重分析显示，实验成功制备了氨基接枝改性的纳米羟基磷灰石材料；透射电镜下可见，氨基接枝改性的纳米羟基磷灰石材料有机溶剂N，N-二甲基甲酰胺中仍能以纳米尺寸分散，稳定的分散状态可以维持超过180 d；②扫描电镜下可见，当复合材料中的改性纳米羟基磷灰石含量为10%，30%时，显示出了优秀的纳米级分散效果；当含量为50%时，改性纳米羟基磷灰石不能均匀分散于聚乳酸体系中；③随着改性纳米羟基磷灰石含量的增加，材料的拉伸强度降低、弹性模量升高，综合考虑下复合材料中改性纳米羟基磷灰石含量为10%时具有较好的机械性能；④CCK8实验显示，氨基接枝改性的纳米羟基磷灰石(含量为10%)/聚乳酸复合材料对小鼠成纤维细胞无明显的毒性；⑤结果表明，氨基接枝改性纳米羟基磷灰石(含量为10%)/聚乳酸复合材料具有良好的机械力学性能与细胞相容性。
缩略语：羟基磷灰石：hydroxyapatite，HA；聚乳酸：polylactic acid，PLA

https://orcid.org/0000-0002-2589-0690(刘龙珠)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 骨替代材料, 羟基磷灰石, 聚乳酸, 骨移植, 羟基磷灰石, 细胞毒性

Abstract: BACKGROUND: Bone graft materials and artificial bone substitutes are often selected for oral maxillofacial defects repair. Bone graft materials have their advantages, but the shortcomings exist such as immune response and insufficient sources. The application potential of artificial bone substitutes as materials for bone defects repair is huge. 
OBJECTIVE: To explore the method of the preparation and characterization of the composites of amino-grafted nano-hydroxyapatite and polylactic acid as oral artificial jaw materials. 
METHODS: The graft-modified nano-hydroxyapatite/polylactic acid composites were prepared based on amino-modified hydroxyapatite for making artificial jaws. The content of graft-modified nano-hydroxyapatite in the composite was 10%, 30%, and 50%, separately. Physical and chemical properties, mechanical properties, and cytocompatibility of the composite were characterized.
RESULTS AND CONCLUSION: (1) The spectroscopy of 1H nuclear magnetic resonance, Fourier transform infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis showed that the amino modifier diethylene glycol amine phosphate monoester of modified hydroxyapatite was successfully prepared. Under the transmission electron microscope, graft-modified nano-hydroxyapatite could still be dispersed in nanometer size in the organic solvent of the N,N-dimethylformamide. The stable dispersion state could be maintained for more than 180 days. (2) Scanning electron microscopy showed that when the content of the graft-modified nano-hydroxyapatite in the composite was 10% and 30%, an excellent nano-level dispersion effect exhibited. When the content was 50%, the modified nano-hydroxyapatite could not be uniformly dispersed in the poly lactic acid system. (3) With the increase of graft-modified nano-hydroxyapatite content, tensile strength decreased and elastic modulus increased. The results showed that graft-modified nano-hydroxyapatite content in the composite had better mechanical properties when the content was 10%. (4) CCK-8 assay results displayed that the composites of amino-grafted nano-hydroxyapatite (10% content) and polylactic acid have no obvious toxicity to mouse fibroblasts. (5) It is concluded that the composites of amino-grafted nano-hydroxyapatite (10% content) and polylactic acid have good mechanical properties and cytocompatibility. 

Key words: bone substitutes, hydroxyapatite, polylactic acid, bone transplantation, hydroxyapatite, cytotoxicity

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