中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (1): 61-66.doi: 10.3969/j.issn.2095-4344.2015.01.011

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脐血干细胞移植及电针治疗脊髓损伤大鼠神经生长因子及神经营养因子3的表达

孙兆忠1，李  瑞1，房清敏1，王光林1，耿晓鹏1，任佳彬1，杨  成2   

  1. 1滨州医学院附属医院，山东省滨州市  256603
    2滨州医学院，山东省烟台市  264003
  • 修回日期:2014-11-29 出版日期:2015-01-01 发布日期:2015-01-01
  • 通讯作者: 杨成，博士，教授，滨州医学院，山东省烟台市 264003
  • 作者简介:孙兆忠，男，1966年生，山东省淄博市人，汉族，主任医师，主要从事脊柱损伤方面的基础与临床研究。
  • 基金资助:

    山东省科技发展计划资助项目(2010GSF10254)，项目名称：电针联合移植脐血干细胞治疗脊髓损伤的实验研究

Expression of nerve growth factor and neurotrophin 3 after transplantation of human umbilical cord blood stem cells combined with electroacupuncture stimulation in rats with spinal cord injuries

Sun Zhao-zhong1, Li Rui1, Fang Qing-min1, Wang Guang-lin1, Geng Xiao-peng1, Ren Jia-bin1, Yang Cheng2   

  1. 1Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China
    2Binzhou Medical University, Yantai 264003, Shandong Province, China
  • Revised:2014-11-29 Online:2015-01-01 Published:2015-01-01
  • Contact: Yang Cheng, M.D., Professor, Binzhou Medical University, Yantai 264003, Shandong Province, China
  • About author:Sun Zhao-zhong, Chief physician, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China

PDF

646

被引次数

10

摘要：

背景：研究表明，脐血干细胞移植对脊髓损伤的恢复起促进作用，而电针也能够通过抑制星形胶质细胞增生，来减少损伤部瘢痕形成，故推测两者结合可能在急性脊髓损伤治疗中发挥重要作用。
目的：观察人脐血干细胞局部移植联合督脉电针治疗后大鼠脊髓损伤组织神经生长因子、神经营养因子3的表达。
方法：选取雌性SD大鼠72只，随机分为对照组、损伤组、移植组、联合组。对照组单纯性背部切口后缝合，损伤组脊髓横断处(T10水平)放置约1 mm×2 mm×2 mm大小、浸润生理盐水的明胶海绵；移植组及联合组在脊髓横断处放置浸润人脐血干细胞悬液的明胶海绵，联合组于造模后1 h开始给予督脉电针治疗。在相应处理7，14，28 d后应用免疫组织化学、Western Blot及实时荧光定量PCR方法检测脊髓组织神经生长因子、神经营养因子3表达量的变化。
结果与结论：脊髓损伤后，移植组与损伤组相比，联合组与移植组相比，神经生长因子、神经营养因子3在7，14，28 d表达量均增加(P < 0.05)。Western Blot、实时荧光定量PCR与免疫组化结果相一致。结果显示人脐血干细胞移植与电针联合治疗脊髓损伤具有协同作用，显著上调损伤脊髓神经生长因子、神经营养因子3的表达水平，有利于脊髓损伤后功能恢复。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 移植, 电针, 人脐血干细胞, 脊髓损伤, 神经生长因子, 神经营养因子3

Abstract:

BACKGROUND: Studies have shown that umbilical cord blood stem cell transplantation promote the recovery of spinal cord injury, and electroacupuncture also can inhibit the proliferation of astrocytes to reduce damage to scar formation, suggesting that a combination of umbilical cord blood stem cell transplantation and electroacupuncture may play an important role in the treatment of acute spinal cord injuries.
OBJECTIVE: To observe the influence of transplantation of human umbilical cord blood stem cells combined with electroacupuncture at the Du channel on expression of nerve growth factor and neurotrophin 3 in rats with spinal cord injuries.
METHODS: Seventy-two female Sprague-Dawlay rats were randomly divided into control group, injury group, transplantation group and combined therapy group. In the control group, only an incision on the back was sutured; in the injury group, a piece of saline-infiltrated gelatin sponge, 1 mm×2 mm×2 mm, was placed into the transected spinal cord at T10 level; in the transplantation group and combined therapy group, a piece of gelatin sponged infiltrated in the suspension of human umbilical cord blood stem cells was placed into the transected spinal cord, respectively, and then, electroacupuncture stimulation at the Du channel was performed in the combined therapy group at 1 hour after modeling. Specimens were taken at 7, 14, 28 days after modeling in each group, and then immunohistochemistry, western blot and real time-PCR methods were used to detect the expression of nerve growth factor and neurotrophin 3.
RESULTS AND CONCLUSION: Compared with the transplantation group, the expression of nerve growth factor and neurotrophin 3 was lower in the injury group but higher in the combined therapy group at 7, 14, 28 days after modeling(P < 0.05). The results of western blot and real time-PCR were consistent with those of immunohistochemical detection. Findings show that human umbilical cord blood stem cell transplantation combined with electroacupuncture has a remarkable synergistic effect in the treatment of spinal cord injury that can significantly up-regulate the expression of nerve growth factor and neurotrophin 3, and contribute to injured spinal cord repair, regeneration and functional recovery after spinal cord injury.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: Cord Blood Stem Cell Transplantation, Electroacupuncture, Spinal Cord Injuries, Nerve Growth Factor, Neurotrophin 3

中图分类号: