中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (52): 8433-8437.doi: 10.3969/j.issn.2095-4344.2014.52.013

• 药物控释材料 drug delivery materials • 上一篇    下一篇

聚羟基脂肪酸酯和聚酰胺-胺树状高分子构筑的经皮给药系统

缪伟伟1,2,王志雄1,3,张俊利3,大野優子2   

  1. 1上海医疗器械高等专科学校,上海市 200093;2日本国立大阪大学,日本大阪府吹田市 565-0871;3上海理工大学,上海市 200093
  • 修回日期:2014-11-01 出版日期:2014-12-17 发布日期:2014-12-17
  • 通讯作者: 大野優子,教授,日本国立大阪大学,日本大阪府吹田市 565-0871
  • 作者简介:缪伟伟,女,1988年生,汉族,日本大阪大学在读博士,上海医疗器械高等专科学校助教,主要从事生物医学工程方面的教学与科研工作。
  • 基金资助:

    上海市教育委员会科研创新项目(自然科学类)(11YZ293),项目名称:用生物合成生分解材料mcl-PHA构筑经皮给药系统

Transdermal drug delivery system constructed by polyhydroxyalkanoates and PAMAM dendrimers

Miao Wei-wei1, 2, Wang Zhi-xiong1, 3, Zhang Jun-li3, Yuko Ohno2   

  1. 1Shanghai Medical Instrumentation College, Shanghai 200093, China; 2Osaka University, Japan 565-0871; 3University of Shanghai for Science and Technology, Shanghai 200093, China
  • Revised:2014-11-01 Online:2014-12-17 Published:2014-12-17
  • Contact: Yuko Ohno, Professor, Osaka University, Japan 565-0871
  • About author:Miao Wei-wei, Studying for doctorate, Assistant teacher, Shanghai Medical Instrumentation College, Shanghai 200093, China; Osaka University, Japan 565-0871
  • Supported by:

    Shanghai Education Commission Scientific Research Innovation Projects (Natural Science Class), No. 11YZ293

摘要:

背景:聚羟基脂肪酸化合物具有黏着性及与药物分子混合、分散的特性,可作为体外给药剂型的基质;树状高分子具有独特的物理、化学性质,能够作为药物的载体。

目的:将聚羟基脂肪酸化合物与聚酰胺-胺树状高分子混合,构筑能够促进难溶型药物的透皮的经皮给药系统。
方法:将恶臭假单胞菌 KT2442在含有NH4Cl的培养基中30 ℃培养 72 h,然后在培养体系中添加5 g/L辛烷酸培养24 h,用氯仿抽提聚羟基脂肪酸化合物。各取10 mg聚羟基脂肪酸化合物基质与含聚酰胺-胺与苯乙胺树状高分子的聚羟基脂肪酸化合物基质,分别与5 mg磺酰胺衍生物Tamsulosin混合,构建经皮给药系统;同时构建体外渗透研究装置,研究基质促进药物渗透的效果。X射线衍射分析Tamsulosin、聚羟基脂肪酸化合物基质、基质聚羟基脂肪酸化合物-Tamsulosin及含聚酰胺-胺树状高分子聚羟基脂肪酸化合物基质- Tamsulosin的内部原子空间分布状况。

结果与结论:聚羟基脂肪酸化合物及聚羟基脂肪酸化合物与聚酰胺-胺树状高分子的混合物都能作为经皮给药的基质,促进难溶药物的透皮作用;并且在相同的条件下,聚羟基脂肪酸化合物与聚酰胺-胺树状高分子的混合物作为基质的经皮给药系统能够满足药物的临床血药浓度。聚羟基脂肪酸化合物与聚酰胺-胺树状高分子的混合物能够使模型药物很好地晶体化并呈现高度有序的方向性。


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 缓释材料, 聚羟基脂肪酸酯, 聚酰胺-胺树状高分子, 经皮给药系统, Tamsulosin

Abstract:

BACKGROUND: Polyhydroxyalkanoates (PHA) can be used as an in vitro drug delivery carrier for its characteristics of viscosity and ability of drug mixture and dispersion. PAMAM dendrimers have unique physical and chemical properties as a drug carrier.

OBJECTIVE: To mix PHA with PAMAM to build a transdermal drug delivery system for indissolvable drugs.
METHODS: Pseudomonas putida KT2442 was cultured in a culture medium containing NH4Cl at 30 for 72 hours, then 5 g/L octane acid was added in the culture system and cultured for 24 hours, and PHA compound was extracted with chloroform. PHA compound matrix (10 mg) and PHA compound matrix containing PAMAM and phenethylamine dendrimers (10 mg) were respectively mixed with 5 mg of sulfonamide derivative Tamsulosin to construct a transdermal delivery system. Meanwhile, an in vitro permeation device was established to study the promoting effect of compound matrices on drug penetration. X-ray diffraction analysis was used to analyze the atomic spatial distribution of Tamsulosin, PHA, PHA compound matrix-Tamsulosin, and PHA compound matrix containing PAMAM dendrimers-Tamsulosin.
RESULTS AND CONCLUSION: PHA and mixture of PHA and PAMAM dendrimers could promote the transdermal effect of indissolvable drugs as a transdermal drug matrix. In the same condition, the mixture of PHA and PAMAM dendrimers could satisfy the clinical blood drug concentration as a transdermal drug matrix. This mixture can make drug model crystallized well in a highly ordered manner.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: polyhydroxyalkanoates, administration, cutaneous, osmosis

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