中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (28): 4444-4449.doi: 10.3969/j.issn.2095-4344.2014.28.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞与肝星状细胞共培养后凋亡相关基因的改变

胡昆鹏1,刘  波1,姚志成1,林继宗2,邓美海2,潘卫东2,林  楠2,陈  骋2,许瑞云2
  

  1. 1中山大学附属第三医院岭南医院普通外科,广东省广州市  510530;2中山大学附属第三医院肝胆外科,广东省广州市  510630
  • 出版日期:2014-07-02 发布日期:2014-07-02
  • 通讯作者: 许瑞云,博士生导师,中山大学附属第三医院肝胆外科,广东省广州市 510630
  • 作者简介:胡昆鹏,男,1982年生,江西省丰城市人,汉族,2010年中山大学毕业,博士,主治医师,主要从事肝硬化、肝癌的基础与临床研究。
  • 基金资助:

    国家教育部博士点基金(20110171120089,20110171110070)

Changes in apoptosis-related genes in bone marrow mesenchymal stem cells after cocultured with hepatic stellate cells

Hu Kun-peng1, Liu Bo1, Yao Zhi-cheng1, Lin Ji-zong2, Deng Mei-hai2, Pan Wei-dong2, Lin Nan2, Chen Cheng2, Xu Rui-yun2   

  1. 1 Department of General Surgery, Lingnan Hospital, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510530, Guangdong Province, China; 2 Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China
  • Online:2014-07-02 Published:2014-07-02
  • Contact: Xu Rui-yun, Doctoral supervisor, Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China
  • About author:Hu Kun-peng, M.D., Attending physician, Department of General Surgery, Lingnan Hospital, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510530, Guangdong Province, China
  • Supported by:

    the Doctoral Fund of Ministry of Education of China, No. 20110171120089, 20110171110070

摘要:

背景:前期研究证实骨髓间充质干细胞可在体外促进肝星状细胞凋亡、抑制其活性,但其作用机制尚未明确。
目的:通过基因芯片技术,筛选出骨髓间充质干细胞调控肝星状细胞凋亡过程中可能参与的凋亡相关基因。
方法:将分离提纯的人骨髓间质干细胞与肝星状细胞在6孔Transwell板上建立上下共培养体系,单独培养人骨髓间质干细胞作为对照组,培养72 h。基因芯片分析单独培养组与共培养组骨髓间充质干细胞凋亡相关基因的改变情况,找出与调控肝星状细胞密切相关的凋亡基因。
结果与结论:采用SABiosciences第2代功能分类基因芯片产品进行凋亡基因筛查发现,共培养后骨髓间质干细胞中显著上调的凋亡基因有:AKT1,PIK3R2,DAPK1,DHCR24,NOTCH2,BDNF等。结合前期研究结果,推测NOTCH有可能在骨髓间充质干细胞调控肝星状细胞过程中起关键作用。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

关键词: 干细胞, 骨髓干细胞, 肝星状细胞, 骨髓间充质干细胞, 凋亡, 肝纤维化, 肝硬化, 基因芯片

Abstract:

BACKGROUND: Previous studies have confirmed that bone marrow mesenchymal stem cells in vitro can promote hepatic stellate cell apoptosis and inhibit its activity, in which the mechanism of action remains unknown.
OBJECTIVE: To screen out apoptosis-related genes during hepatic stellate cell apoptosis regulated by bone marrow mesenchymal stem cells using gene chip technology.
METHODS: Purified human bone marrow mesenchymal stem cells were seeded in 6-well Transwell plate and cocultured with hepatic stellate cells. Cultured human bone marrow mesenchymal stem cells alone served as control group, and cultured for 72 hours. The alterations in apoptosis-related genes were analyzed between culture alone group and coculture group using gene chip technology. The genes strongly associated with regulation of hepatic stellate cells were selected. RESULTS AND CONCLUSION: By the functional classification of second-generation SABiosciences Gene chips, apoptotic gene screening found that after coculture, significantly upregulated genes in bone marrow mesenchymal stem cells contained: AKT1, PIK3R2, DAPK1, DHCR24, NOTCH2 and BDNF. Combined with previous findings, we hypothesized that NOTCH may play a key role in the regulation of hepatic stellate cells by bone marrow mesenchymal stem cells.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

Key words: bone marrow, mesenchymal stem cells, hepatic stellate cells, apoptosis, microchip analytical , procedures

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