中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3715-3719.doi: 10.3969/j.issn.1673-8225.2012.20.024

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

微小RNA-146a影响血管平滑肌细胞增殖和凋亡的机制*☆◆

熊  玮1，董少红1，袁建辉2，刘建军2，徐新云2，李江华1   

  1. 1暨南大学第二临床医学院 深圳市人民医院心内科，广东省深圳市  518020；2深圳市疾病预防与控制中心 深圳市现代毒理学重点实验室，广东省深圳市 518020
  • 收稿日期:2012-03-01 修回日期:2012-03-19 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 董少红，博士，教授，暨南大学第二临床医学院 深圳市人民医院心内科，广东省深圳市 518020 dsh266@medmail.com.cn
  • 作者简介:熊玮☆，男，1975年生，广东省深圳市人，汉族，暨南大学第二临床医学院在读博士，主治医师，主要从事冠状动脉粥样硬化性心脏病及冠状动脉介入后再狭窄防治的研究。 xw1012@sina.com
  • 基金资助:

    深圳市科技计划项目(201102158)。

Essential role of microRNA-146a in proliferation and apoptosis of vascular smooth muscle cells 

Xiong Wei1, Dong Shao-hong1, Yuan Jian-hui2, Liu Jian-jun2, Xu Xin-yun2, Li Jiang-hua1   

  1. 1Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen  518020, Guangdong Province, China; 2Key Laboratory of Modern Toxicology in Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen  518020, Guangdong Province, China
  • Received:2012-03-01 Revised:2012-03-19 Online:2012-05-13 Published:2012-05-13
  • Contact: Dong Shao-hong, Doctor, Professor, Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong Province, China dsh266@medmail.com.cn
  • About author:Xiong Wei☆, Studying for doctorate, Attending physician, Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong Province, China xw1012@sina.com
  • Supported by:

     the Science and Technology Plan of Shenzhen City, No. 201102158*

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摘要：

背景：微小RNA-146a通过负调控核因子κB信号通路介导免疫细胞和肿瘤细胞的增殖，但是否参与血管平滑肌细胞的增殖或凋亡未见研究报道。
目的：观察微小RNA-146a对血管平滑肌细胞增殖、凋亡的影响及其相关机制。 
方法：采用脂质体2000将50 nmol/L微小RNA-146a反义寡核苷酸转染至大鼠血管平滑肌细胞，以转染错义链及PBS的细胞作为对照。 
结果与结论：微小RNA-146a反义寡核苷酸转染48 h后，血管平滑肌细胞增殖减少(P < 0.01)、凋亡增多(P < 0.05)，细胞的微小RNA-146a水平明显降低(P < 0.01)，核因子κBp65、增殖细胞核抗原蛋白表达明显降低(P < 0.05)。说明微小RNA-146a可以促进血管平滑肌细胞的增殖，抑制凋亡，其机制与增加核因子κBp65表达有关。
关键词：微小RNA-146a；血管平滑肌细胞；核因子κB；转染；增殖；凋亡
doi:10.3969/j.issn.1673-8225.2012.20.024

关键词: 微小RNA-146a, 血管平滑肌细胞, 核因子&kappa, B, 转染, 增殖, 凋亡

Abstract:

BACKGROUND: MicroRNA-146a (miRNA-146a) can mediate the proliferation of immune cells and tumor cells through negative regulation of nuclear factor κB signaling pathway, but whether miRNA-146a participates in the proliferation or apoptosis of vascular smooth muscle cells (VSMCs) has not been reported.
OBJECTIVE: To investigate the role of miRNA-146a in VSMCs proliferation and apoptosis and to exploit its mechanisms.
METHODS: Artificial synthesized miRNA-146a antisense oligonucleotide (ASO, 50 nmol/L), scramble (control, 50nmol/l) and phosphate buffered saline (normal) were transfected into VSMCs by Lipofectamine 2000 individually.
RESULTS AND CONCLUSION: By the end of 48 hours of transfection, there were significantly lower levels of miRNA-146a mRNA in ASO treated VSMCs compared with that in normal and control VSMCs (P < 0.01). Meanwhile, ASO treated VSMCs manifested a lower proliferative (P < 0.01) and higher apoptotic ability (P < 0.05). The protein level of nuclear factor-κBp65 and proliferation cell nuclear antigen in ASO treated VSMCs were remarkably lower than that in normal and control VSMCs (P < 0.05). miRNA-146a is capable of promoting proliferation and suppressing apoptosis of VMSCs, which is probably related with the increase in nuclear factor-κBp65 expression.

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